LOCUS       pABDP2                  9158 bp    DNA     circular SYN 09-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     Sequence Label: pABDP2
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             97..165
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             802..1461
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1559..2241
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    2400..3199
                     /label=psbH downstream flanking element
     misc_feature    complement(2417..2550)
                     /label=trnE2
     primer_bind     3138..3158
                     /label=Bac Col pcr FW
     terminator      complement(3200..3602)
                     /label=psbA/ 3' UTR
     CDS             complement(3603..4322)
                     /codon_start=1
                     /label=mVenus
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     4283..4342
                     /label=mVenus petB fw
     primer_bind     complement(4303..4362)
                     /label=mVenus-petB Rv
     promoter        4323..4955
                     /label=BDP2
     primer_bind     4916..4975
                     /label=petB-tdTomat FW
     primer_bind     complement(4936..4995)
                     /label=petB-tdTomat RV
     CDS             4956..6386
                     /codon_start=1
                     /label=td Tomato
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(5706..5721)
                     /label=petB-tdTomat RV
     terminator      6387..6799
                     /locus_tag="rbcL 3' UTR element "
                     
                     
     primer_bind     complement(6392..6411)
                     /label=bac col pcr rv
     misc_feature    6800..9158
                     /locus_tag="psbH upstream flanking element"
                     /label=psbH upstream flanking element
     CDS             complement(6945..7211)
                     /codon_start=1
                     /locus_tag="psbH"
                     /label=psbH
                     /translation="MATGTSKAKPSKVNSDFQEPGLVTPLGTLLRPLNSEAGKVLPGWG
                     TTVLMAVFILLFAAFLLIILEIYNSSLILDDVSMSWETLAKVS"
     CDS             7781..7915
                     /codon_start=1
                     /label=psbN
                     /translation="MESPAFFFTFFLWFLLLSVTGYSVYVSFGPPSKKLRDPFEEHED"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcttac
     3241 ggaatataaa tattagtcga gtggtaccgc cactgcctag tatataaata tcggcagttg
     3301 gcaggatatt tatatactcc gaaggaactt gttagccgat aggcgaggca actgccacta
     3361 aaatttattt gcctcctaac ggagcattaa aatccctaag tttacttgcc cgtaagggga
     3421 aggggacgtc cactaatatt tatattaggc agttggcagg caacaataaa tacatttgtc
     3481 ccgtaagggg acgtcctgcc aactgcctat ggtagctatt aagtatatat atatgaaaag
     3541 tgtgtataaa ctaaaataaa ccaggtatgg ttaaccagat ttattttagt ttaaaaaaaa
     3601 atttatttat ataattcatc catacctaat gtaataccag cagctgttac aaattctaat
     3661 aaaaccatat gatcacgttt ttcatttgga tcttttgata atttagattg ataactaaga
     3721 taatgattat caggtaataa aacaggacca tctccaattg gtgtattttg ttgataatga
     3781 tcagctaatt gaacaccacc atcttcaata ttatgacgaa ttttaaaatt agctttgata
     3841 ccatttttct gtttatcagc tgtaatataa acattatgtg aattataatt atattctaat
     3901 ttatgaccta aaatattacc atcttcttta aaatcaatac ctttaagttc aatacgatta
     3961 actaatgtat ctccttcaaa tttaacttca gcacgtgttt tataattacc atcatcttta
     4021 aagaaaattg tacgttcttg tacataacct tcaggcattg cacttttaaa gaaatcatgt
     4081 tgtttcatgt gatctggata acgagcaaaa cattgtaaac cataacctaa tgtagtaact
     4141 aaagttggcc atggaactgg taatttacct gttgtacaaa ttaattttaa agttaattta
     4201 ccataagttg cgtcaccttc accttcacca ctaactgaaa atttgtgacc attaacgtca
     4261 ccatctaatt caactaaaat tggaactaca ccagtgaata attcttcacc ttttgaaacc
     4321 attataaaat ttttataaga ttgtgacatg accattaggc tttcttaaga ctaaaaaaat
     4381 gtgtagctta attatttaaa gtttaaatta aagttataat atatattata tataaaataa
     4441 aaaaaacgtt agtaattcaa aagttttaat attatacaat tgaactatta tgtattaaat
     4501 ataagaatgt cacctcttac catatttcta tactccaaag taacttttta cataaatgtc
     4561 ccctctgggg ctgcctcctt ccccttcccc ttcggtatat aaatataggg caagtaaact
     4621 tagcataaac tttagttgcc cgaaggggtt tacatactcc gaaggaggac aaatttattt
     4681 attgtggtac aataaataaa ttgtatgtaa acccctttcg ggtaactaaa gtttatcacg
     4741 gcaataagtt tctgcttacg cagtattata tctgacgcag tattatataa gaagttggca
     4801 ggataaaaat gtgtaagtat ggcaatcttt taaaatagtg ttcaattcat ttaaggcaga
     4861 taaaaagaaa aaagtccaca ggatttaatt ttgaatagtt ctctatcaaa aaaaggtttg
     4921 ccgaacaatg tttttattcc tggagtttga ttttaatggt atctaaaggt gaagaagtaa
     4981 ttaaggaatt catgcgtttt aaagttcgta tggaaggtag tatgaatggt catgaatttg
     5041 aaattgaagg tgagggtgaa ggtcgtccat atgaaggtac acaaactgct aagttaaaag
     5101 taactaaagg tggtccatta ccatttgcat gggatatttt atcaccacaa ttcatgtatg
     5161 gttcaaaagc ttatgtaaaa cacccagctg atattcctga ctataaaaaa ttatcatttc
     5221 cagaaggttt taaatgggaa cgtgttatga actttgaaga tggtggttta gtaacagtaa
     5281 ctcaagattc tagtttacaa gatggtacat taatatataa agttaaaatg cgtggtacta
     5341 attttccacc tgatggtcca gtaatgcaga aaaagacaat gggttgggaa gcttcaactg
     5401 aacgtttata tccacgtgat ggtgtactaa aaggtgaaat tcatcaagca cttaaactta
     5461 aagatggtgg tcattactta gtagaattca aaacaatcta tatggctaaa aaacctgtac
     5521 aattaccagg ttattattat gttgatacta aattagatat tactagtcat aatgaagatt
     5581 atacaattgt agaacaatat gaacgttcag aaggtcgtca tcatctgttt ttaggtcatg
     5641 gtactggatc tacaggttca ggtagtagtg gtacagcttc ttctgaagat aataatatgg
     5701 ctgttattaa ggaattcatg cgttttaaag taagaatgga aggtagtatg aatggtcatg
     5761 aatttgaaat tgaaggtgaa ggtgaaggta gaccatatga aggtacacaa actgctaagt
     5821 taaaagttac aaaaggtggt ccattacctt ttgcttggga tattttaagt ccacaattca
     5881 tgtatggttc taaagcttat gttaaacatc cagctgatat tccagattat aaaaaattaa
     5941 gttttcctga aggttttaaa tgggaacgtg taatgaattt tgaagatggt ggtttagtta
     6001 cagttacaca agacagttct ttacaagatg gtactttaat ctataaagtt aaaatgagag
     6061 gtactaattt tccacctgat ggtccagtaa tgcaaaagaa aacaatgggt tgggaagcaa
     6121 gtacagaacg tttatatcct cgtgatggtg ttttaaaagg tgaaattcac caagctttaa
     6181 agttaaaaga tggtggtcac tacttagtag agtttaaaac aatatatatg gctaaaaaac
     6241 ctgtacaatt accaggttac tattatgttg atactaaatt agatattaca tcacataatg
     6301 aagattatac aattgttgaa caatatgaac gtagtgaagg tcgtcatcac ttatttttat
     6361 atggtatgga tgaattatat aaataagtac tcaagctcgt aacgaaggtc gtgaccttgc
     6421 tcgtgaaggt ggcgacgtaa ttcgttcagc ttgtaaatgg tctccagaac ttgctgctgc
     6481 atgtgaagtt tggaaagaaa ttaaattcga atttgatact attgacaaac tttaattttt
     6541 atttttcatg atgtttatgt gaatagcata aacatcgttt ttatttttat ggtgtttagg
     6601 ttaaatacct aaacatcatt ttacattttt aaaattaagt tctaaagtta tcttttgttt
     6661 aaatttgcct gtctttataa attacgatgt gccagaaaaa taaaatctta gctttttatt
     6721 atagaattta tctttatgta ttatatttta taagttataa taaaagaaat agtaacatac
     6781 taaagcggat gtagcgcgtt tatcttaacg gaaggccagt ggcagtggcg gtgccactgc
     6841 cgaatataaa tatggttgag ttgcttagtt taccttagcg aaaagaagac ttagcagcta
     6901 gccttaacaa acagttttat attttatgtt tgtgttaaat aaaattaaga aactttagct
     6961 aaagtttccc aactcataga aacgtcatct aaaattaaag aactgttgta aatttctaaa
     7021 atgattaata agaatgctgc aaataaaagg ataaatacag ccattaaaac agttgtaccc
     7081 cagcctggta atactttacc tgcttctgag ttaagtggac gtaataaagt acctaatggt
     7141 gtaactaaac caggttcttg gaagtctgaa tttacttttg atggtttagc tttagaagtt
     7201 cctgttgcca taattgatta aatgaattaa gcgttattag cgctatttta tttactttct
     7261 gtaaaaaata aggaaaatat tcttcagtgc attccctctc aggattataa atactctgag
     7321 gataacgttc tctcgtcaag gggttgcttc ttgtgagtat agaaacctac tagcacaaga
     7381 aataaattgc ataaaaatgt atttacctag gaccgcagta ggcagtccct tttccccttc
     7441 agaactgcct gctttaaaag aatgaaaaaa ctgccttgtc tggtaagtaa aactctttaa
     7501 ttactcacta aagacgatct tagaagttct ttgttcattt tttatttaat ataatatttg
     7561 ttatataaaa attaaataat ttttaattaa tgtttaactt tgtaaggaca gtttcaaagt
     7621 gacatgaatg gctactgcaa aaacgaagta agttattctt tctcagggca aaattttgag
     7681 tagattaatt ttgtttaaaa atgtgggaca cagtcgtcaa gtcttttgaa ctatctaaga
     7741 gatatgttga aaagagaata attttattat taaatgagct atggaaagtc cagctttttt
     7801 ctttaccttt tttttatggt ttcttctgtt aagtgtaact ggctattcag tttatgttag
     7861 ttttggtcca ccttcaaaaa aattacgtga tccttttgaa gaacacgaag attaaacaag
     7921 ttaaaaagta ctatttttac aagtgacttc ggtgcctctg agaaccctag ttatagtgat
     7981 ataaaataac tagctaacta ctttatattt ttatgaaagt cattttgtcg agcatataaa
     8041 caaaaacaaa attgctatac taggcagtca cagtgcaact gtctccgtct ccttaaccga
     8101 gaaagggtaa acgtcttcgg taaagtaaca aactttagtt atgttaactg cttgcgagtt
     8161 aaccattttt tttcctccga aggacaacag ttggcagttg ccaaacttta gtggtctaat
     8221 atttatatta ggcagttggc aggcaactgc actgacgtcc cgaaggggaa ggggtttact
     8281 tacctcctaa cggagtatat aaatagaata aaatttattt cctgcgctag cagatttaca
     8341 tactaggatt ttaatactcc gaaggaggca gtggcggtac cactgccact ggcgtcctcc
     8401 ttccccttcg ggcaaatgca ttttagtgcc acttaagttt acttgcctag gcagttggca
     8461 ggacgtcagt ggcagtggta ccgcgactgc ctatatttat atactcctaa gtttacttgc
     8521 ctaggcagtt ggcaggcaac tgccactgac gtccttcccc ttccccttcg ggacgtcccc
     8581 ttacgggaat ataaatatta gtggatattt atatactgcg atgtttacat actccgaagg
     8641 aggagagcta gcagttgcct gccaactgcc taatataaat attgggcaag taaacttaga
     8701 atgtttacat actccgaagg aggacgtccc ttacgggaat ataaatatta gtggcagtgg
     8761 taccgccact gcctccttcg gagtattaaa atcctagtat ataatatacc gtaagggacg
     8821 tcctccgacg gtggcagtgg cggtaccact gccaccggcg tcctaatata catattgaag
     8881 tatttaaacc tgttagcgca cgctctaacg agtcagtaaa cttccctttt ggggcttcta
     8941 ggcagcgcat aaattttcta ggacgaacgt ccactggcgt ctcgtaagga gcagtgacag
     9001 gccactaatg tccccttaat gggtaaataa atggctatcg tctatccatg aagagaccat
     9061 atattccagt agcaccgtta tgatcctcaa agggtaacac catttgtata gtattatggt
     9121 gaaatgcatc cctttcaggg tagatttata tcttacag
//
