LOCUS       pABDP3                  9096 bp    DNA     circular SYN 09-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     Sequence Label: pABDP3
FEATURES             Location/Qualifiers
     source          1..9096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             97..165
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             802..1461
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1559..2241
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    2400..3199
                     /label=psbH downstream flanking element
     misc_feature    complement(2417..2550)
                     /label=trnE2
     terminator      complement(3200..3602)
                     /label=psbA/ 3' UTR
     CDS             complement(3603..4322)
                     /codon_start=1
                     /label=mVenus
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     4283..4342
                     /label=mVenus psbN fw
     primer_bind     complement(4303..4362)
                     /label=mVenus-psbN Rv
     promoter        4323..4893
                     /label=BDP3
     primer_bind     4323..4369
                     /label=P psbN-psbH FW
     primer_bind     4854..4913
                     /label=psbN-tdTomat FW
     primer_bind     complement(4874..4933)
                     /label=psbN-Td tomato RV
     CDS             4894..6324
                     /codon_start=1
                     /label=td Tomato
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(5644..5659)
                     /label=psbN-Td tomato RV
     terminator      6325..6737
                     /locus_tag="rbcL 3' UTR element "
                     
                     
     misc_feature    6738..9096
                     /locus_tag="psbH upstream flanking element"
                     /label=psbH upstream flanking element
     CDS             complement(6883..7149)
                     /codon_start=1
                     /locus_tag="psbH"
                     /label=psbH
                     /translation="MATGTSKAKPSKVNSDFQEPGLVTPLGTLLRPLNSEAGKVLPGWG
                     TTVLMAVFILLFAAFLLIILEIYNSSLILDDVSMSWETLAKVS"
     primer_bind     7149..7195
                     /label=P psbN-psbH FW
     primer_bind     complement(7149..7188)
                     /label=mVenus-psbN Rv
     primer_bind     7680..7719
                     /label=psbN-tdTomat FW
     primer_bind     complement(7700..7719)
                     /label=psbN-Td tomato RV
     CDS             7719..7853
                     /codon_start=1
                     /label=psbN
                     /translation="MESPAFFFTFFLWFLLLSVTGYSVYVSFGPPSKKLRDPFEEHED"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcttac
     3241 ggaatataaa tattagtcga gtggtaccgc cactgcctag tatataaata tcggcagttg
     3301 gcaggatatt tatatactcc gaaggaactt gttagccgat aggcgaggca actgccacta
     3361 aaatttattt gcctcctaac ggagcattaa aatccctaag tttacttgcc cgtaagggga
     3421 aggggacgtc cactaatatt tatattaggc agttggcagg caacaataaa tacatttgtc
     3481 ccgtaagggg acgtcctgcc aactgcctat ggtagctatt aagtatatat atatgaaaag
     3541 tgtgtataaa ctaaaataaa ccaggtatgg ttaaccagat ttattttagt ttaaaaaaaa
     3601 atttatttat ataattcatc catacctaat gtaataccag cagctgttac aaattctaat
     3661 aaaaccatat gatcacgttt ttcatttgga tcttttgata atttagattg ataactaaga
     3721 taatgattat caggtaataa aacaggacca tctccaattg gtgtattttg ttgataatga
     3781 tcagctaatt gaacaccacc atcttcaata ttatgacgaa ttttaaaatt agctttgata
     3841 ccatttttct gtttatcagc tgtaatataa acattatgtg aattataatt atattctaat
     3901 ttatgaccta aaatattacc atcttcttta aaatcaatac ctttaagttc aatacgatta
     3961 actaatgtat ctccttcaaa tttaacttca gcacgtgttt tataattacc atcatcttta
     4021 aagaaaattg tacgttcttg tacataacct tcaggcattg cacttttaaa gaaatcatgt
     4081 tgtttcatgt gatctggata acgagcaaaa cattgtaaac cataacctaa tgtagtaact
     4141 aaagttggcc atggaactgg taatttacct gttgtacaaa ttaattttaa agttaattta
     4201 ccataagttg cgtcaccttc accttcacca ctaactgaaa atttgtgacc attaacgtca
     4261 ccatctaatt caactaaaat tggaactaca ccagtgaata attcttcacc ttttgaaacc
     4321 attaattgat taaatgaatt aagcgttatt agcgctattt tatttacttt ctgtaaaaaa
     4381 taaggaaaat attcttcagt gcattccctc tcaggattat aaatactctg aggataacgt
     4441 tctctcgtca aggggttgct tcttgtgagt atagaaacct actagcacaa gaaataaatt
     4501 gcataaaaat gtatttacct aggaccgcag taggcagtcc cttttcccct tcagaactgc
     4561 ctgctttaaa agaatgaaaa aactgccttg tctggtaagt aaaactcttt aattactcac
     4621 taaagacgat cttagaagtt ctttgttcat tttttattta atataatatt tgttatataa
     4681 aaattaaata atttttaatt aatgtttaac tttgtaagga cagtttcaaa gtgacatgaa
     4741 tggctactgc aaaaacgaag taagttattc tttctcaggg caaaattttg agtagattaa
     4801 ttttgtttaa aaatgtggga cacagtcgtc aagtcttttg aactatctaa gagatatgtt
     4861 gaaaagagaa taattttatt attaaatgag ctaatggtat ctaaaggtga agaagtaatt
     4921 aaggaattca tgcgttttaa agttcgtatg gaaggtagta tgaatggtca tgaatttgaa
     4981 attgaaggtg agggtgaagg tcgtccatat gaaggtacac aaactgctaa gttaaaagta
     5041 actaaaggtg gtccattacc atttgcatgg gatattttat caccacaatt catgtatggt
     5101 tcaaaagctt atgtaaaaca cccagctgat attcctgact ataaaaaatt atcatttcca
     5161 gaaggtttta aatgggaacg tgttatgaac tttgaagatg gtggtttagt aacagtaact
     5221 caagattcta gtttacaaga tggtacatta atatataaag ttaaaatgcg tggtactaat
     5281 tttccacctg atggtccagt aatgcagaaa aagacaatgg gttgggaagc ttcaactgaa
     5341 cgtttatatc cacgtgatgg tgtactaaaa ggtgaaattc atcaagcact taaacttaaa
     5401 gatggtggtc attacttagt agaattcaaa acaatctata tggctaaaaa acctgtacaa
     5461 ttaccaggtt attattatgt tgatactaaa ttagatatta ctagtcataa tgaagattat
     5521 acaattgtag aacaatatga acgttcagaa ggtcgtcatc atctgttttt aggtcatggt
     5581 actggatcta caggttcagg tagtagtggt acagcttctt ctgaagataa taatatggct
     5641 gttattaagg aattcatgcg ttttaaagta agaatggaag gtagtatgaa tggtcatgaa
     5701 tttgaaattg aaggtgaagg tgaaggtaga ccatatgaag gtacacaaac tgctaagtta
     5761 aaagttacaa aaggtggtcc attacctttt gcttgggata ttttaagtcc acaattcatg
     5821 tatggttcta aagcttatgt taaacatcca gctgatattc cagattataa aaaattaagt
     5881 tttcctgaag gttttaaatg ggaacgtgta atgaattttg aagatggtgg tttagttaca
     5941 gttacacaag acagttcttt acaagatggt actttaatct ataaagttaa aatgagaggt
     6001 actaattttc cacctgatgg tccagtaatg caaaagaaaa caatgggttg ggaagcaagt
     6061 acagaacgtt tatatcctcg tgatggtgtt ttaaaaggtg aaattcacca agctttaaag
     6121 ttaaaagatg gtggtcacta cttagtagag tttaaaacaa tatatatggc taaaaaacct
     6181 gtacaattac caggttacta ttatgttgat actaaattag atattacatc acataatgaa
     6241 gattatacaa ttgttgaaca atatgaacgt agtgaaggtc gtcatcactt atttttatat
     6301 ggtatggatg aattatataa ataagtactc aagctcgtaa cgaaggtcgt gaccttgctc
     6361 gtgaaggtgg cgacgtaatt cgttcagctt gtaaatggtc tccagaactt gctgctgcat
     6421 gtgaagtttg gaaagaaatt aaattcgaat ttgatactat tgacaaactt taatttttat
     6481 ttttcatgat gtttatgtga atagcataaa catcgttttt atttttatgg tgtttaggtt
     6541 aaatacctaa acatcatttt acatttttaa aattaagttc taaagttatc ttttgtttaa
     6601 atttgcctgt ctttataaat tacgatgtgc cagaaaaata aaatcttagc tttttattat
     6661 agaatttatc tttatgtatt atattttata agttataata aaagaaatag taacatacta
     6721 aagcggatgt agcgcgttta tcttaacgga aggccagtgg cagtggcggt gccactgccg
     6781 aatataaata tggttgagtt gcttagttta ccttagcgaa aagaagactt agcagctagc
     6841 cttaacaaac agttttatat tttatgtttg tgttaaataa aattaagaaa ctttagctaa
     6901 agtttcccaa ctcatagaaa cgtcatctaa aattaaagaa ctgttgtaaa tttctaaaat
     6961 gattaataag aatgctgcaa ataaaaggat aaatacagcc attaaaacag ttgtacccca
     7021 gcctggtaat actttacctg cttctgagtt aagtggacgt aataaagtac ctaatggtgt
     7081 aactaaacca ggttcttgga agtctgaatt tacttttgat ggtttagctt tagaagttcc
     7141 tgttgccata attgattaaa tgaattaagc gttattagcg ctattttatt tactttctgt
     7201 aaaaaataag gaaaatattc ttcagtgcat tccctctcag gattataaat actctgagga
     7261 taacgttctc tcgtcaaggg gttgcttctt gtgagtatag aaacctacta gcacaagaaa
     7321 taaattgcat aaaaatgtat ttacctagga ccgcagtagg cagtcccttt tccccttcag
     7381 aactgcctgc tttaaaagaa tgaaaaaact gccttgtctg gtaagtaaaa ctctttaatt
     7441 actcactaaa gacgatctta gaagttcttt gttcattttt tatttaatat aatatttgtt
     7501 atataaaaat taaataattt ttaattaatg tttaactttg taaggacagt ttcaaagtga
     7561 catgaatggc tactgcaaaa acgaagtaag ttattctttc tcagggcaaa attttgagta
     7621 gattaatttt gtttaaaaat gtgggacaca gtcgtcaagt cttttgaact atctaagaga
     7681 tatgttgaaa agagaataat tttattatta aatgagctat ggaaagtcca gcttttttct
     7741 ttaccttttt tttatggttt cttctgttaa gtgtaactgg ctattcagtt tatgttagtt
     7801 ttggtccacc ttcaaaaaaa ttacgtgatc cttttgaaga acacgaagat taaacaagtt
     7861 aaaaagtact atttttacaa gtgacttcgg tgcctctgag aaccctagtt atagtgatat
     7921 aaaataacta gctaactact ttatattttt atgaaagtca ttttgtcgag catataaaca
     7981 aaaacaaaat tgctatacta ggcagtcaca gtgcaactgt ctccgtctcc ttaaccgaga
     8041 aagggtaaac gtcttcggta aagtaacaaa ctttagttat gttaactgct tgcgagttaa
     8101 ccattttttt tcctccgaag gacaacagtt ggcagttgcc aaactttagt ggtctaatat
     8161 ttatattagg cagttggcag gcaactgcac tgacgtcccg aaggggaagg ggtttactta
     8221 cctcctaacg gagtatataa atagaataaa atttatttcc tgcgctagca gatttacata
     8281 ctaggatttt aatactccga aggaggcagt ggcggtacca ctgccactgg cgtcctcctt
     8341 ccccttcggg caaatgcatt ttagtgccac ttaagtttac ttgcctaggc agttggcagg
     8401 acgtcagtgg cagtggtacc gcgactgcct atatttatat actcctaagt ttacttgcct
     8461 aggcagttgg caggcaactg ccactgacgt ccttcccctt ccccttcggg acgtcccctt
     8521 acgggaatat aaatattagt ggatatttat atactgcgat gtttacatac tccgaaggag
     8581 gagagctagc agttgcctgc caactgccta atataaatat tgggcaagta aacttagaat
     8641 gtttacatac tccgaaggag gacgtccctt acgggaatat aaatattagt ggcagtggta
     8701 ccgccactgc ctccttcgga gtattaaaat cctagtatat aatataccgt aagggacgtc
     8761 ctccgacggt ggcagtggcg gtaccactgc caccggcgtc ctaatataca tattgaagta
     8821 tttaaacctg ttagcgcacg ctctaacgag tcagtaaact tcccttttgg ggcttctagg
     8881 cagcgcataa attttctagg acgaacgtcc actggcgtct cgtaaggagc agtgacaggc
     8941 cactaatgtc cccttaatgg gtaaataaat ggctatcgtc tatccatgaa gagaccatat
     9001 attccagtag caccgttatg atcctcaaag ggtaacacca tttgtatagt attatggtga
     9061 aatgcatccc tttcagggta gatttatatc ttacag
//
