LOCUS       2528.pLV-Puro-EF       14519 bp    DNA     circular SYN 11-NOV-2025
DEFINITION  .
ACCESSION   VB231201-1259kxx
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14519
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
                     /note="color: #512bbd; direction: RIGHT"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
                     /note="color: #d84e4e; direction: RIGHT"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
                     /note="color: #1f36a9; direction: RIGHT"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="color: #db6901; direction: RIGHT"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
                     /note="color: #800c0c; direction: RIGHT"
     promoter        1959..3137
                     /label=EF1A
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation 
                     elongationfactor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="color: #0c5d90; direction: RIGHT"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="color: #f58600; direction: RIGHT"
     CDS             3168..6980
                     /label={HA-PARP1-3xGS-TurboC(ns)}
                     /note="/application_notes="
                     /note="/official_designation={HA-PARP1-3xGS-TurboC(ns)}"
                     /note="/uuid=5b01e88d59a7454385c91e359bb367b0"
                     /note="color: #6ddaae; direction: RIGHT"
     misc_feature    6981..7043
                     /label=T2A
                     /label=nonstandard type:  Linker
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=Self-cleaving 2A peptide from Thosea 
                     asignavirus"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
                     /note="color: #5566f5; direction: RIGHT"
     CDS             7044..9191
                     /label={XRCC1-myc-3xGS-TurboN}
                     /note="/application_notes="
                     /note="/official_designation={XRCC1-myc-3xGS-TurboN}"
                     /note="/uuid=6436bf2391cd4f9586bb1ca6db2df7d0"
                     /note="color: #12f548; direction: RIGHT"
     misc_feature    9230..9827
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #ef6cdf; direction: RIGHT"
     promoter        9846..10356
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
                     /note="color: #c54b7c; direction: RIGHT"
     CDS             10369..10968
                     /label=Puro
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
                     /note="color: #d05c0a; direction: RIGHT"
     LTR             11039..11273
                     /label=3' LTR-Delta-U3
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
                     /note="color: #fd3434; direction: RIGHT"
     polyA_signal    11346..11480
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
                     /note="color: #46c6ef; direction: RIGHT"
     CDS             12434..13294
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="color: #eda481; direction: RIGHT"
     rep_origin      13465..14053
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
                     /note="color: #da3bb5"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tacccatacg
     3181 atgttccaga ttacgctgcg gagtcttcgg ataagctcta tcgagtcgag tacgccaaga
     3241 gcgggcgcgc ctcttgcaag aaatgcagcg agagcatccc caaggactcg ctccggatgg
     3301 ccatcatggt gcagtcgccc atgtttgatg gaaaagtccc acactggtac cacttctcct
     3361 gcttctggaa ggtgggccac tccatccggc accctgacgt tgaggtggat gggttctctg
     3421 agcttcggtg ggatgaccag cagaaagtca agaagacagc ggaagctgga ggagtgacag
     3481 gcaaaggcca ggatggaatt ggtagcaagg cagagaagac tctgggtgac tttgcagcag
     3541 agtatgccaa gtccaacaga agtacgtgca aggggtgtat ggagaagata gaaaagggcc
     3601 aggtgcgcct gtccaagaag atggtggacc cggagaagcc acagctaggc atgattgacc
     3661 gctggtacca tccaggctgc tttgtcaaga acagggagga gctgggtttc cggcccgagt
     3721 acagtgcgag tcagctcaag ggcttcagcc tccttgctac agaggataaa gaagccctga
     3781 agaagcagct cccaggagtc aagagtgaag gaaagagaaa aggcgatgag gtggatggag
     3841 tggatgaagt ggcgaagaag aaatctaaaa aagaaaaaga caaggatagt aagcttgaaa
     3901 aagccctaaa ggctcagaac gacctgatct ggaacatcaa ggacgagcta aagaaagtgt
     3961 gttcaactaa tgacctgaag gagctactca tcttcaacaa gcagcaagtg ccttctgggg
     4021 agtcggcgat cttggaccga gtagctgatg gcatggtgtt cggtgccctc cttccctgcg
     4081 aggaatgctc gggtcagctg gtcttcaaga gcgatgccta ttactgcact ggggacgtca
     4141 ctgcctggac caagtgtatg gtcaagacac agacacccaa ccggaaggag tgggtaaccc
     4201 caaaggaatt ccgagaaatc tcttacctca agaaattgaa ggttaaaaaa caggaccgta
     4261 tattcccccc agaaaccagc gcctccgtgg cggccacgcc tccgccctcc acagcctcgg
     4321 ctcctgctgc tgtgaactcc tctgcttcag cagataagcc attatccaac atgaagatcc
     4381 tgactctcgg gaagctgtcc cggaacaagg atgaagtgaa ggccatgatt gagaaactcg
     4441 gggggaagtt gacggggacg gccaacaagg cttccctgtg catcagcacc aaaaaggagg
     4501 tggaaaagat gaataagaag atggaggaag taaaggaagc caacatccga gttgtgtctg
     4561 aggacttcct ccaggacgtc tccgcctcca ccaagagcct tcaggagttg ttcttagcgc
     4621 acatcttgtc cccttggggg gcagaggtga aggcagagcc tgttgaagtt gtggccccaa
     4681 gagggaagtc aggggctgcg ctctccaaaa aaagcaaggg ccaggtcaag gaggaaggta
     4741 tcaacaaatc tgaaaagaga atgaaattaa ctcttaaagg aggagcagct gtggatcctg
     4801 attctggact ggaacactct gcgcatgtcc tggagaaagg tgggaaggtc ttcagtgcca
     4861 cccttggcct ggtggacatc gttaaaggaa ccaactccta ctacaagctg cagcttctgg
     4921 aggacgacaa ggaaaacagg tattggatat tcaggtcctg gggccgtgtg ggtacggtga
     4981 tcggtagcaa caaactggaa cagatgccgt ccaaggagga tgccattgag cacttcatga
     5041 aattatatga agaaaaaacc gggaacgctt ggcactccaa aaatttcacg aagtatccca
     5101 aaaagttcta ccccctggag attgactatg gccaggatga agaggcagtg aagaagctga
     5161 cagtaaatcc tggcaccaag tccaagctcc ccaagccagt tcaggacctc atcaagatga
     5221 tctttgatgt ggaaagtatg aagaaagcca tggtggagta tgagatcgac cttcagaaga
     5281 tgcccttggg gaagctgagc aaaaggcaga tccaggccgc atactccatc ctcagtgagg
     5341 tccagcaggc ggtgtctcag ggcagcagcg actctcagat cctggatctc tcaaatcgct
     5401 tttacaccct gatcccccac gactttggga tgaagaagcc tccgctcctg aacaatgcag
     5461 acagtgtgca ggccaaggtg gaaatgcttg acaacctgct ggacatcgag gtggcctaca
     5521 gtctgctcag gggagggtct gatgatagca gcaaggatcc catcgatgtc aactatgaga
     5581 agctcaaaac tgacattaag gtggttgaca gagattctga agaagccgag atcatcagga
     5641 agtatgttaa gaacactcat gcaaccacac acaatgcgta tgacttggaa gtcatcgata
     5701 tctttaagat agagcgtgaa ggcgaatgcc agcgttacaa gccctttaag cagcttcata
     5761 accgaagatt gctgtggcac gggtccagga ccaccaactt tgctgggatc ctgtcccagg
     5821 gtcttcggat agccccgcct gaagcgcccg tgacaggcta catgtttggt aaagggatct
     5881 atttcgctga catggtctcc aagagtgcca actactgcca tacgtctcag ggagacccaa
     5941 taggcttaat cctgttggga gaagttgccc ttggaaacat gtatgaactg aagcacgctt
     6001 cacatatcag caagttaccc aagggcaagc acagtgtcaa aggtttgggc aaaactaccc
     6061 ctgatccttc agctaacatt agtctggatg gtgtagacgt tcctcttggg accgggattt
     6121 catctggtgt gaatgacacc tctctactat ataacgagta cattgtctat gatattgctc
     6181 aggtaaatct gaagtatctg ctgaaactga aattcaattt taagacctcc ctgtggggac
     6241 agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc
     6301 tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag
     6361 ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta
     6421 tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg
     6481 tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg
     6541 acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag
     6601 gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg
     6661 aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga
     6721 atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg
     6781 aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg
     6841 tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg
     6901 gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc
     6961 tgcggtctgc cgaaaaggct ggaagcggag agggcagggg aagtcttcta acatgcgggg
     7021 acgtggagga aaatcccggc cccatgccgg agatccgcct ccgccatgtc gtgtcctgca
     7081 gcagccagga ctcgactcac tgtgcagaaa atcttctcaa ggcagacact taccgaaaat
     7141 ggcgggcagc caaggcaggc gagaagacca tctctgtggt cctacagttg gagaaggagg
     7201 agcagataca cagtgtggac attgggaatg atggctcagc tttcgtggag gtgctggtgg
     7261 gcagttcagc tggaggcgct ggggagcaag actatgaggt ccttctggtc acctcatctt
     7321 tcatgtcccc ttccgagagc cgcagtggct caaaccccaa ccgcgttcgc atgtttgggc
     7381 ctgacaagct ggtccgggca gccgccgaga agcgctggga ccgggtcaaa attgtttgca
     7441 gccagcccta cagcaaggac tccccctttg gcttgagttt tgtacggttt catagccccc
     7501 cagacaaaga tgaggcagag gccccgtccc agaaggtgac agtgaccaag cttggccagt
     7561 tccgtgtgaa ggaggaggat gagagcgcca actctctgag gccgggggct ctcttcttca
     7621 gccggatcaa caagacatcc ccagtcacag ccagcgaccc agcaggacct agctatgcag
     7681 ctgctaccct ccaggcttct agtgctgcct cctcagcctc tccagtctcc agggccatag
     7741 gcagcacctc caagccccag gagtctccca aagggaagag gaagttggat ttgaaccaag
     7801 aagaaaagaa gacccccagc aaaccaccag cccagctgtc gccatctgtt cccaagagac
     7861 ctaaattgcc agctccaact cgtaccccag ccacagcccc agtccctgcc cgagcacagg
     7921 gggcagtgac aggcaaaccc cgaggagaag gcaccgagcc cagacgaccc cgagctggcc
     7981 cagaggagct ggggaagatc cttcagggtg tggtagtggt gctgagtggc ttccagaacc
     8041 ccttccgctc cgagctgcga gataaggccc tagagcttgg ggccaagtat cggccagact
     8101 ggacccggga cagcacgcac ctcatctgtg cctttgccaa cacccccaag tacagccagg
     8161 tcctaggcct gggaggccgc atcgtgcgta aggagtgggt gctggactgt caccgcatgc
     8221 gtcggcggct gccctcccag aggtacctca tggcagggcc aggttccagc agtgaggagg
     8281 atgaggcctc tcacagcggt ggcagcggag atgaagcccc caagcttcct cagaagcaac
     8341 cccagaccaa aaccaagccc actcaggcag ctggacccag ctcaccccag aagcccccaa
     8401 cccctgaaga gaccaaagca gcctcaccag tgctccagga agatatagac attgaggggg
     8461 tacagtcaga aggacaggac aatggggcgg aagattctgg ggacacagag gatgagctga
     8521 ggagggtggc agagcagaag gaacacagac tgccccctgg ccaggaggag aatggggaag
     8581 acccgtatgc aggctccacg gatgagaaca cggacagtga ggaacaccag gagcctcctg
     8641 atctgccagt ccctgagctc ccagatttct tccagggcaa gcacttcttt ctttacgggg
     8701 agttccctgg ggacgagcgg cggaaactca tccgatacgt cacagccttc aatggggagc
     8761 tcgaggacta tatgagtgac cgggttcagt ttgtgatcac agcacaggaa tgggatccca
     8821 gctttgagga ggccctgatg gacaacccct ccctggcatt cgttcgtccc cgatggatct
     8881 acagttgcaa tgagaagcag aagttacttc ctcaccagct ctatggggtg gtgccgcaag
     8941 ccgaacaaaa actcatctca gaagaggatc tgaaagacaa tactgtgcct ctgaagctga
     9001 tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa accctgggca
     9061 tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc gtggacgtgt
     9121 tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg aacgctaaac
     9181 agattctgtg aacccagctt tcttgtacaa agtggtgata atcgaattcc gataatcaac
     9241 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9301 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9361 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9421 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9481 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9541 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9601 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     9661 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9721 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9781 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt
     9841 tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     9901 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     9961 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
    10021 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
    10081 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
    10141 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
    10201 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    10261 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    10321 tgttctcctc ttcctcatct ccgggccttt cgacctcacg tggccaccat gaccgagtac
    10381 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10441 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    10501 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    10561 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    10621 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10681 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10741 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10801 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    10861 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10921 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg tacctttaag
    10981 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    11041 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
    11101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    11281 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    11341 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11461 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
    11521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11641 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    11701 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    11761 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    11821 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    11881 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    11941 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    12001 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    12061 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    12121 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    12181 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    12241 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    12301 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    12361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    12421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    12481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    12541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    12601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    12661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    12721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    12781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    12841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    12901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    12961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    13021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    13081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    13141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    13201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    13261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    13321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    13381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    13441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    13501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    13561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    13621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    13681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    13741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    13801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    13861 agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa gcggcagggt
    13921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    13981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    14041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    14101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    14161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    14221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    14281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    14341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    14401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    14461 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
