LOCUS       pMB-pTrcHis2A-Ucdh-export        8125 bp    DNA     circular UNA 25-JUL-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...n0-i05jwhm
VERSION     urn.local...n0-i05jwhm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(193..198,199..215,216..222)
                     /note="strong <i>E. coli</i> promoter; hybrid between the
                     <i>trp</i> and <i>lac</i>&nbsp;UV5 promoters"
                     /standard_name="trc promoter"
     protein_bind    230..246
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     misc_feature    263..332
                     /note="antiterminator from the <i>E. coli rrnG</i> leader
                     region&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/2479752""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/2479752"">(Berg
                     et al., 1989)</a>"
                     /standard_name="rrnG antiterminator"
     misc_feature    346..354
                     /note="translational enhancer from gene <i>10</i> of
                     bacteriophage T7&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/2676996""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/2676996"">(Olin
                     s and Rangwala, 1989)</a>"
                     /standard_name="gene 10 translational enhancer"
     RBS             369..373
     CDS             383..409
                     /codon_start=1
                     /note="This first cistron should terminate 3 bp upstream
                     of the ATG for the second cistron."
                     /product="synthetic cistron containing a ribosome binding
                     site (Shine-Dalgarno sequence), for enhancing the
                     bacterial expression of a downstream cistron&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/9108515""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/9108515"">(Scho
                     ner, 1997)</a>"
                     /transl_table=1
                     /translation="MYRLNKEE*"
                     /standard_name="minicistron"
     misc_feature    391..4207
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="EcdhSML(NHis) (concatenated sequence 2)"
     misc_feature    join(4183,411..>415)
                     /note="multiple cloning site"
                     /standard_name="MCS"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /transl_table=1
                     /translation="M"
                     /standard_name="ATG"
     misc_feature    415^416
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATG"
     source          complement(<416..>4182)
                     /organism="Enterocloster bolteae"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA-613"
                     /culture_collection="ATCC:BAA-613"
                     /type_material="type strain of Clostridium bolteae"
                     /db_xref="taxon:208479"
                     /collection_date="2016-08-01"
     misc_feature    <416..>4182
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pET28a-Ecdh_XdhC(NHis6)_wprimer - source
                     Enterocloster bolteae (concatenated sequence 2)"
     misc_feature    complement(<416..>4182)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Ecdh (concatenated sequence 2) (reversed)"
     CDS             <416..1282
                     /locus_tag="CGC65_RS20430"
                     /old_locus_tag="CGC65_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="WP_002569575.1"
                     /db_xref="GeneID:61860681"
                     /codon_start=3
                     /standard_name="xanthine dehydrogenase family protein
                     subunit M CDS"
     CDS             1296..1787
                     /locus_tag="CGC65_RS20425"
                     /old_locus_tag="CGC65_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_002569574.1"
                     /db_xref="GeneID:61860680"
                     /translation="MKHSISLIINGDPVDAIVKDNLTLLDFLRDQLFLTGTKKGCEEG
                     ECGACTVMLDGKPVNSCCTLAVECDGHEIITVEGIAREGMLHPIQKQFIEKWAMQCGY
                     CTPGMIMSAKALLDVNKHPTELEIREAIEGNLCRCTGYAKIVEAIQAAAAQMNWEEEA
                     KNA"
                     /standard_name="(2Fe-2S)-binding protein CDS"
     CDS             1780..4182
                     /locus_tag="CGC65_RS20420"
                     /old_locus_tag="CGC65_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_002569573.1"
                     /db_xref="GeneID:61860679"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     source          complement(<1789..>1827)
                     /organism="Ellagibacter isourolithinifaciens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="DSM104140T_contig_001"
                     /strain="DSM 104140"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="DSM:104140"
                     /type_material="type strain of Ellagibacter
                     isourolithinifaciens"
                     /db_xref="taxon:2137581"
                     /country="Spain: Murcia"
                     /collection_date="2016-06-15"
     source          complement(<1789..>1827)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     misc_feature    <1789..>1827
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Gedh3 (concatenated sequence 2)"
     misc_feature    4170..>4182
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="XdhC (concatenated sequence 3)"
     misc_feature    4183..415
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pTrcHis2 A (digested by BamHI, HindIII)
                     Fragment 2 (concatenated sequence 1)"
     CDS             4190..4219
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /standard_name="Myc"
     CDS             4235..4252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /standard_name="6xHis"
     terminator      4478..4564
     terminator      4656..4683
     promoter        4703..4794
     CDS             join(4795..4863,4864..5655)
                     /codon_start=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /standard_name="AmpR"
     rep_origin      5826..6414
     promoter        6926..7003
     CDS             7004..8086
                     /codon_start=1
                     /gene="<i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli. </i>This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /product="<i>lac</i> repressor"
                     /transl_table=1
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /standard_name="lacI"
ORIGIN      
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggtatt
      421 acctcaattc gagtatcttg ctcctaagac catcggggag gcgtgcaatc tgttccttga
      481 gcttggaagc acagccaggg ttatggcagg cgcaacggat ctgattccgc caatgaaaga
      541 caaggttata tcaccagagt atatcattga ccttaagaaa attccaggtt tggattatct
      601 ggaatacgat gacagggaag gtttaaaaat aggggcgctg acaacactgc gtaccataga
      661 gacatctcct ctggttaaag aaaagaatcc ggcagtggct catgccgcca aggtagtggc
      721 atccacacaa atcaggacaa agggcaccat ggcaggcaat atctgcaatg cgtccccatc
      781 ctgcgataca gcccccaacc tactggctca gggcgctaag atattggtac agggtcccaa
      841 caaggaccgg gtcattcaaa tcgaagactt tttcctgggt gtcaaaaaga cttccctgga
      901 gccgggagaa atcgtgacag gcattgtgat tccgccgctg gctgagaatg agagggctgc
      961 atacataaaa cacgcggtca gaaaggcgat ggacctggcc atcatcggcg tcgcagtcaa
     1021 gattaaggtg gaagacggcg tctgcacaga cgccaggatt gccctgggcg cggttgcggc
     1081 cacgccggta cgggcacccg gggcggagga agccctgatt ggcaaagaac ttaccgatga
     1141 agtgattgtg aaggcatcgg aggaagcaat gaattcctgt catccaatct ctgatatccg
     1201 cgcatcggcc gaatacagga aggatatgat ccgcgtgttt acaaaacgtg ccattaagca
     1261 ggccatggaa tgctataact aggagggaga caggaatgaa acatagtata agtttaatca
     1321 tcaacggaga tccggttgat gctattgtga aggataacct gacgcttctg gattttcttc
     1381 gtgaccagtt gtttctgaca ggcacgaaaa aggggtgtga ggaaggggag tgtggcgcct
     1441 gtaccgtgat gcttgacggt aagccggtta actcctgctg taccctggcc gtggagtgcg
     1501 acggccatga gatcataacc gtggagggaa ttgccaggga ggggatgctt catcccattc
     1561 agaagcagtt tatagagaag tgggccatgc agtgcggcta ctgtactccg ggtatgatta
     1621 tgtccgccaa agctcttctg gatgtgaata aacatcctac ggaactggaa atccgggaag
     1681 ccattgaggg caatctgtgc cgctgcaccg gttatgcaaa gattgtggag gcaatccagg
     1741 cagccgctgc gcagatgaac tgggaggagg aagcaaagaa tgcataaaca tcaccatcac
     1801 catcacgact acgacatccc gactaccgac tgtgacaaac attattttaa aaaaccggaa
     1861 ttttaccgtc tgaccggcga gaacaattat gtcaggattg acgctgaaga caaggtaaca
     1921 ggacacggcc agtatgtggg tgacatcatg tttccggata tgcttaccgg aaaaatggtc
     1981 agaagccctt atgcatctgc aaaaatcctg tccattgaca ccagcgaggc agagaagctt
     2041 ccaggtgtaa agtgcatcct cactgccagg gactttgagt ggaagtccct ggtgggaaat
     2101 ggagaatttg cagctgagtt cgcggacaag gaagtattgt gttctgagaa ggtgcgccag
     2161 gttggtgacg atgtggcagc cgtggcagcc gtggatgagg aaaccgccca gcgggccgcg
     2221 gatctgatta aggtagagta ccaggtgctt ccgggggtgt ttgacccctt tgaggcaatg
     2281 gaggagaatg cccccgaggt aaactgggag ggcaaaggca tacacaatat cggcatgcag
     2341 tccgtgatga aggcgggcac ggatattgat gaggaatttg accgcgcatc ctatgtgcag
     2401 cacagggatt acaagaccca ccgtatggta catgcggcca tggagcccca cggtgcggtg
     2461 gctacctaca ggaatgggac ctacaccatc tggatgtcca cccagatgtc ctttgtggac
     2521 cagttctggt atgcccgctg ccttggcgtt ggagagaacc aggtgcgggt aatcaagcct
     2581 cttgtgggcg gcggtttcgg cggcaagctg gattcctatt cctttggcct ttgcgctgcc
     2641 aagatggcgg agatgaccgg acgcccggta cgcatgattc tgtcccgcga agaagtattc
     2701 cagaccacgc gcaaccgcca ccccatttac atgcatattg acactgcctt tgggacagac
     2761 ggtaagctgc tggcaaagaa atgctaccat gtgctggacg gcgggcctta cggcgggtcc
     2821 ggtgttgcgg cctgcgccca gtccacattg tgggccaact tcccttacaa gatgaattcc
     2881 gtggatttcc tggcaaggcg tgtgtatacc aataaccctt ctgcaggagc catgagagga
     2941 tatacggcat gccaggtgca ttttgcccat gacctgaaca tgcagttcgc ggctgaccag
     3001 atgggcattg acccggttga gttccgcaag ataagcgcgg cagaccccgg atacgtggct
     3061 ccggccggac tggcaatcac cagctgcgca tacaaggaga cactggatac ggctgcaaag
     3121 gaaattggct ggtatgagaa gaaggataag ctgaaaaagg gagagggcat tggttttgcg
     3181 ggcaccggct ttgtatcggg caccggattc gcggtgctgg aagcgcccaa ccagagctct
     3241 gcgtgcgtga ccctgcgcat gaacaagcgg ggcatggcca ccctgtacat cggctcccac
     3301 gacataggac agggttccga tacggttatg acggccattg tggccgagga actggggctt
     3361 cccatggata tggtcaagac ctttatgtcc gacaccttcc tgacgccctg ggactccggc
     3421 tcttacggca gccgtgttac cttcctggcc ggcaatgcgg cccgccgcgc tgccgtggat
     3481 gcaaaacgcc agctgtttga ggtcattgcg cctatgtggg gggtgatgcc ggagacatta
     3541 gagtgtctgg acggaaaggt aatcagcaag gagaaggcag agtatcagat gtccattgga
     3601 gacgccatgt tcaagtatat gacggtcaag ggcggcgatg aactgattgg cgtgggatcc
     3661 tattaccacc gtaccgacaa ttcacagtat aacggcaaca ataccaccaa ctacgcgcct
     3721 gcatacagct tctccaccgg agcagcccat ctgactgtgg acgaggagac cggcgtcctg
     3781 gatattgatg aatttgtatt tgcccatgac tgcggccgcg cactgaataa gagggccgtg
     3841 gaaggccagc tggagggatc catcggcatg ggcctgggct atgctgtcta tgagcacaat
     3901 gtaaccaggg aaggaaagat tctcaatccc aacttccgcg attaccggct gcccactgcc
     3961 cttgatatgc cgaagatgcg caccttttac gactttacac cggacgagga aggacctctg
     4021 ggcgcaaagg aagctggcga gggctccgca gcacctgtgg cgcctgccat tgccaatgcg
     4081 gtcaacatgg caaccggcgt gtacttcaca gagctgcctc tggacccgga gcatatctgg
     4141 agagcgctgc acggcatgaa ggatgacagg aattcaaaat aatgggcccg aacaaaaact
     4201 catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc attgagttta
     4261 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
     4321 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     4381 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     4441 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     4501 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     4561 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     4621 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     4681 ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg
     4741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4801 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4861 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4921 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4981 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5041 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5101 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5161 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5221 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5281 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5341 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5401 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5461 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5521 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5581 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5641 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5701 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5761 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5941 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6601 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     6661 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     6721 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6781 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6841 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
     6901 aggcgaagcg gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat
     6961 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     7021 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     7081 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     7141 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     7201 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     7261 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     7321 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     7381 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     7441 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     7501 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     7561 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     7621 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     7681 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     7741 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     7801 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtc
     7861 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     7921 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     7981 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8041 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8101 atgtgagtta gcgcgaattg atctg
//
