LOCUS       pMB-pTrcHis2A-Ucdh-XdhC        9178 bp    DNA     circular UNA 15-JUL-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...f9-gz6we7m
VERSION     urn.local...f9-gz6we7m
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(193..198,199..215,216..222)
                     /note="strong <i>E. coli</i> promoter; hybrid between the
                     <i>trp</i> and <i>lac</i>&nbsp;UV5 promoters"
                     /standard_name="trc promoter"
     protein_bind    230..246
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     misc_feature    263..332
                     /note="antiterminator from the <i>E. coli rrnG</i> leader
                     region&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/2479752""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/2479752"">(Berg
                     et al., 1989)</a>"
                     /standard_name="rrnG antiterminator"
     misc_feature    346..354
                     /note="translational enhancer from gene <i>10</i> of
                     bacteriophage T7&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/2676996""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/2676996"">(Olin
                     s and Rangwala, 1989)</a>"
                     /standard_name="gene 10 translational enhancer"
     RBS             369..373
     CDS             383..409
                     /codon_start=1
                     /note="This first cistron should terminate 3 bp upstream
                     of the ATG for the second cistron."
                     /product="synthetic cistron containing a ribosome binding
                     site (Shine-Dalgarno sequence), for enhancing the
                     bacterial expression of a downstream cistron&nbsp;<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/9108515""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/9108515"">(Scho
                     ner, 1997)</a>"
                     /transl_table=1
                     /translation="MYRLNKEE*"
                     /standard_name="minicistron"
     misc_feature    391..415
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    391..5260
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="EcdhSML(NHis)_XdhC (concatenated sequence
                     2)"
     misc_feature    join(5236,411..>415)
                     /note="multiple cloning site"
                     /standard_name="MCS"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /transl_table=1
                     /translation="M"
                     /standard_name="ATG"
     primer_bind     416..442
                     /standard_name="oMB-Ucdh-F"
     misc_feature    complement(<416..4195)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Ecdh (concatenated sequence 2) (reversed)"
     source          complement(<416..>4182)
                     /organism="Enterocloster bolteae"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA-613"
                     /culture_collection="ATCC:BAA-613"
                     /type_material="type strain of Clostridium bolteae"
                     /db_xref="taxon:208479"
                     /collection_date="2016-08-01"
     gene            <416..1282
                     /locus_tag="CGC65_RS20430"
                     /old_locus_tag="CGC65_20430"
                     /db_xref="GeneID:61860681"
                     /standard_name="gene"
     CDS             <416..1282
                     /codon_start=1
                     /locus_tag="CGC65_RS20430"
                     /old_locus_tag="CGC65_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="WP_002569575.1"
                     /db_xref="GeneID:61860681"
                     /translation="VLPQFEYLAPKTIGEACNLFLELGSTARVMAGATDLIPPMKDKV
                     ISPEYIIDLKKIPGLDYLEYDDREGLKIGALTTLRTIETSPLVKEKNPAVAHAAKVVA
                     STQIRTKGTMAGNICNASPSCDTAPNLLAQGAKILVQGPNKDRVIQIEDFFLGVKKTS
                     LEPGEIVTGIVIPPLAENERAAYIKHAVRKAMDLAIIGVAVKIKVEDGVCTDARIALG
                     AVAATPVRAPGAEEALIGKELTDEVIVKASEEAMNSCHPISDIRASAEYRKDMIRVFT
                     KRAIKQAMECYN"
                     /standard_name="xanthine dehydrogenase family protein
                     subunit M CDS"
     gene            1296..1787
                     /locus_tag="CGC65_RS20425"
                     /old_locus_tag="CGC65_20425"
                     /db_xref="GeneID:61860680"
                     /standard_name="gene"
     CDS             1296..1787
                     /locus_tag="CGC65_RS20425"
                     /old_locus_tag="CGC65_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_002569574.1"
                     /db_xref="GeneID:61860680"
                     /translation="MKHSISLIINGDPVDAIVKDNLTLLDFLRDQLFLTGTKKGCEEG
                     ECGACTVMLDGKPVNSCCTLAVECDGHEIITVEGIAREGMLHPIQKQFIEKWAMQCGY
                     CTPGMIMSAKALLDVNKHPTELEIREAIEGNLCRCTGYAKIVEAIQAAAAQMNWEEEA
                     KNA"
                     /standard_name="(2Fe-2S)-binding protein CDS"
     gene            1780..4182
                     /locus_tag="CGC65_RS20420"
                     /old_locus_tag="CGC65_20420"
                     /db_xref="GeneID:61860679"
                     /standard_name="gene"
     CDS             1780..4182
                     /locus_tag="CGC65_RS20420"
                     /old_locus_tag="CGC65_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_002569573.1"
                     /db_xref="GeneID:61860679"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     misc_feature    1789..1827
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <1789..>1827
                     /note="Geneious type: Expression casette"
                     /standard_name="expression cassette"
     misc_feature    <1789..>1827
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Gedh3 (concatenated sequence 2)"
     source          complement(<1789..>1827)
                     /organism="Ellagibacter isourolithinifaciens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="DSM104140T_contig_001"
                     /strain="DSM 104140"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="DSM:104140"
                     /type_material="type strain of Ellagibacter
                     isourolithinifaciens"
                     /db_xref="taxon:2137581"
                     /country="Spain: Murcia"
                     /collection_date="2016-06-15"
     source          complement(<1789..>1827)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     misc_feature    1789..1806
                     /standard_name="His6 affinity tag"
     gene            <1789..>1827
                     /locus_tag="BN3560_RS12865"
                     /db_xref="GeneID:47671095"
                     /standard_name="gene"
     gene            <1789..>1827
                     /locus_tag="F8C90_RS00245"
                     /old_locus_tag="F8C90_00245"
                     /standard_name="gene"
     CDS             <1789..>1827
                     /codon_start=1
                     /locus_tag="F8C90_RS00245"
                     /old_locus_tag="F8C90_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_158048609.1"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     misc_feature    1807..1827
                     /standard_name="linker"
     misc_feature    4170..4195
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4170..>5235
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="XdhC (concatenated sequence 3)"
     misc_feature    4182^4183
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AGGCAGTTCAGCGGATGATCAGATGAAACCAGGAAGGCGGCT
                     TCATGCCGCCTTC"
     source          complement(<4183..>5235)
                     /organism="Enterocloster bolteae"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA-613"
                     /culture_collection="ATCC:BAA-613"
                     /type_material="type strain of Clostridium bolteae"
                     /db_xref="taxon:208479"
                     /collection_date="2016-08-01"
     source          complement(<4183..>4203)
                     /organism="Ellagibacter isourolithinifaciens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="DSM104140T_contig_001"
                     /strain="DSM 104140"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="DSM:104140"
                     /type_material="type strain of Ellagibacter
                     isourolithinifaciens"
                     /db_xref="taxon:2137581"
                     /country="Spain: Murcia"
                     /collection_date="2016-06-15"
     source          complement(<4183..>4203)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     misc_feature    4183..4203
                     /standard_name="linker"
     gene            4204..5235
                     /locus_tag="CGC65_RS20280"
                     /old_locus_tag="CGC65_20280"
                     /db_xref="GeneID:61860652"
                     /standard_name="XdhC"
     CDS             4204..5235
                     /locus_tag="CGC65_RS20280"
                     /old_locus_tag="CGC65_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="WP_002565223.1"
                     /db_xref="GeneID:61860652"
                     /translation="MKTLFTELRQLLEQGEEAVLVTIIASSGSTPRGTGSRMLVRKDG
                     SIEGTVGGGAVEYQAIQAALKAMEDKVSFAKGFTLTRNQVADIGMVCGGNVVVYFQYI
                     RPGDQVLTGFCGQVLDALSKDEDSWLILDITDETCWQMGLYSPSLGLSGIKGLGQLLE
                     GELFGSNAFQKEVDGRTFYIEPLVQAGTVYIFGGGHVAQELVPVLAHVGFRCVVMDDR
                     EAFANPQVFLRAERTVVGDMEHISDYVDIRPRDYVCVMTRGHQFDYYVQKQAMALHPY
                     YIGIMGSRNKIRVVTDKLLADGFSLEEIQKCHMPIGTDIGAETPAEIAISIAGELIAR
                     RAERMGKKK"
                     /standard_name="XdhC family protein CDS"
     primer_bind     complement(5208..5235)
                     /standard_name="oMB-XdhC-R"
     misc_feature    5236..5260
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    5236..415
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pTrcHis2 A (digested by BamHI, HindIII)
                     Fragment 2 (concatenated sequence 1)"
     CDS             5243..5272
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /standard_name="Myc"
     CDS             5288..5305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /standard_name="6xHis"
     terminator      5531..5617
     terminator      5709..5736
     promoter        5756..5847
     CDS             join(5848..5916,5917..6708)
                     /codon_start=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /standard_name="AmpR"
     rep_origin      6879..7467
     promoter        7979..8056
     CDS             8057..9139
                     /codon_start=1
                     /gene="<i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli. </i>This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /product="<i>lac</i> repressor"
                     /transl_table=1
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /standard_name="lacI"
ORIGIN      
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggtatt
      421 acctcaattc gagtatcttg ctcctaagac catcggggag gcgtgcaatc tgttccttga
      481 gcttggaagc acagccaggg ttatggcagg cgcaacggat ctgattccgc caatgaaaga
      541 caaggttata tcaccagagt atatcattga ccttaagaaa attccaggtt tggattatct
      601 ggaatacgat gacagggaag gtttaaaaat aggggcgctg acaacactgc gtaccataga
      661 gacatctcct ctggttaaag aaaagaatcc ggcagtggct catgccgcca aggtagtggc
      721 atccacacaa atcaggacaa agggcaccat ggcaggcaat atctgcaatg cgtccccatc
      781 ctgcgataca gcccccaacc tactggctca gggcgctaag atattggtac agggtcccaa
      841 caaggaccgg gtcattcaaa tcgaagactt tttcctgggt gtcaaaaaga cttccctgga
      901 gccgggagaa atcgtgacag gcattgtgat tccgccgctg gctgagaatg agagggctgc
      961 atacataaaa cacgcggtca gaaaggcgat ggacctggcc atcatcggcg tcgcagtcaa
     1021 gattaaggtg gaagacggcg tctgcacaga cgccaggatt gccctgggcg cggttgcggc
     1081 cacgccggta cgggcacccg gggcggagga agccctgatt ggcaaagaac ttaccgatga
     1141 agtgattgtg aaggcatcgg aggaagcaat gaattcctgt catccaatct ctgatatccg
     1201 cgcatcggcc gaatacagga aggatatgat ccgcgtgttt acaaaacgtg ccattaagca
     1261 ggccatggaa tgctataact aggagggaga caggaatgaa acatagtata agtttaatca
     1321 tcaacggaga tccggttgat gctattgtga aggataacct gacgcttctg gattttcttc
     1381 gtgaccagtt gtttctgaca ggcacgaaaa aggggtgtga ggaaggggag tgtggcgcct
     1441 gtaccgtgat gcttgacggt aagccggtta actcctgctg taccctggcc gtggagtgcg
     1501 acggccatga gatcataacc gtggagggaa ttgccaggga ggggatgctt catcccattc
     1561 agaagcagtt tatagagaag tgggccatgc agtgcggcta ctgtactccg ggtatgatta
     1621 tgtccgccaa agctcttctg gatgtgaata aacatcctac ggaactggaa atccgggaag
     1681 ccattgaggg caatctgtgc cgctgcaccg gttatgcaaa gattgtggag gcaatccagg
     1741 cagccgctgc gcagatgaac tgggaggagg aagcaaagaa tgcataaaca tcaccatcac
     1801 catcacgact acgacatccc gactaccgac tgtgacaaac attattttaa aaaaccggaa
     1861 ttttaccgtc tgaccggcga gaacaattat gtcaggattg acgctgaaga caaggtaaca
     1921 ggacacggcc agtatgtggg tgacatcatg tttccggata tgcttaccgg aaaaatggtc
     1981 agaagccctt atgcatctgc aaaaatcctg tccattgaca ccagcgaggc agagaagctt
     2041 ccaggtgtaa agtgcatcct cactgccagg gactttgagt ggaagtccct ggtgggaaat
     2101 ggagaatttg cagctgagtt cgcggacaag gaagtattgt gttctgagaa ggtgcgccag
     2161 gttggtgacg atgtggcagc cgtggcagcc gtggatgagg aaaccgccca gcgggccgcg
     2221 gatctgatta aggtagagta ccaggtgctt ccgggggtgt ttgacccctt tgaggcaatg
     2281 gaggagaatg cccccgaggt aaactgggag ggcaaaggca tacacaatat cggcatgcag
     2341 tccgtgatga aggcgggcac ggatattgat gaggaatttg accgcgcatc ctatgtgcag
     2401 cacagggatt acaagaccca ccgtatggta catgcggcca tggagcccca cggtgcggtg
     2461 gctacctaca ggaatgggac ctacaccatc tggatgtcca cccagatgtc ctttgtggac
     2521 cagttctggt atgcccgctg ccttggcgtt ggagagaacc aggtgcgggt aatcaagcct
     2581 cttgtgggcg gcggtttcgg cggcaagctg gattcctatt cctttggcct ttgcgctgcc
     2641 aagatggcgg agatgaccgg acgcccggta cgcatgattc tgtcccgcga agaagtattc
     2701 cagaccacgc gcaaccgcca ccccatttac atgcatattg acactgcctt tgggacagac
     2761 ggtaagctgc tggcaaagaa atgctaccat gtgctggacg gcgggcctta cggcgggtcc
     2821 ggtgttgcgg cctgcgccca gtccacattg tgggccaact tcccttacaa gatgaattcc
     2881 gtggatttcc tggcaaggcg tgtgtatacc aataaccctt ctgcaggagc catgagagga
     2941 tatacggcat gccaggtgca ttttgcccat gacctgaaca tgcagttcgc ggctgaccag
     3001 atgggcattg acccggttga gttccgcaag ataagcgcgg cagaccccgg atacgtggct
     3061 ccggccggac tggcaatcac cagctgcgca tacaaggaga cactggatac ggctgcaaag
     3121 gaaattggct ggtatgagaa gaaggataag ctgaaaaagg gagagggcat tggttttgcg
     3181 ggcaccggct ttgtatcggg caccggattc gcggtgctgg aagcgcccaa ccagagctct
     3241 gcgtgcgtga ccctgcgcat gaacaagcgg ggcatggcca ccctgtacat cggctcccac
     3301 gacataggac agggttccga tacggttatg acggccattg tggccgagga actggggctt
     3361 cccatggata tggtcaagac ctttatgtcc gacaccttcc tgacgccctg ggactccggc
     3421 tcttacggca gccgtgttac cttcctggcc ggcaatgcgg cccgccgcgc tgccgtggat
     3481 gcaaaacgcc agctgtttga ggtcattgcg cctatgtggg gggtgatgcc ggagacatta
     3541 gagtgtctgg acggaaaggt aatcagcaag gagaaggcag agtatcagat gtccattgga
     3601 gacgccatgt tcaagtatat gacggtcaag ggcggcgatg aactgattgg cgtgggatcc
     3661 tattaccacc gtaccgacaa ttcacagtat aacggcaaca ataccaccaa ctacgcgcct
     3721 gcatacagct tctccaccgg agcagcccat ctgactgtgg acgaggagac cggcgtcctg
     3781 gatattgatg aatttgtatt tgcccatgac tgcggccgcg cactgaataa gagggccgtg
     3841 gaaggccagc tggagggatc catcggcatg ggcctgggct atgctgtcta tgagcacaat
     3901 gtaaccaggg aaggaaagat tctcaatccc aacttccgcg attaccggct gcccactgcc
     3961 cttgatatgc cgaagatgcg caccttttac gactttacac cggacgagga aggacctctg
     4021 ggcgcaaagg aagctggcga gggctccgca gcacctgtgg cgcctgccat tgccaatgcg
     4081 gtcaacatgg caaccggcgt gtacttcaca gagctgcctc tggacccgga gcatatctgg
     4141 agagcgctgc acggcatgaa ggatgacagg aattcaaaat aagactacga catcccgact
     4201 accatgaaaa cattatttac agagttaaga caattactgg aacaggggga ggaagctgtc
     4261 ctggtgacta tcattgccag ttccggctcc actcccaggg gaaccggatc ccggatgctg
     4321 gtaaggaagg acgggtccat agaaggaacc gtgggcgggg gcgccgtgga gtaccaggca
     4381 atacaggcag cccttaaggc catggaggac aaggtttcct ttgccaaagg attcacactt
     4441 accaggaacc aggtggcgga tatcggaatg gtgtgcggag gaaatgtggt ggtatatttc
     4501 cagtacatcc gtcccggcga ccaggtgctg accggtttct gcggccaggt cctggacgcc
     4561 ctctcaaagg atgaggacag ctggcttata ctggatatca ccgacgagac atgctggcag
     4621 atgggccttt acagccccag tctggggctg tccggtatta aggggctggg acagctgtta
     4681 gagggggaac ttttcggcag caatgctttc cagaaggaag tggatggacg aacgttttat
     4741 atcgagcccc tggtacaggc cgggactgtc tatatattcg gaggcggtca tgtggcccag
     4801 gagctggttc cggttctggc ccatgtggga ttccgctgcg tggttatgga tgacagggag
     4861 gcgtttgcca atccccaggt gtttttgcgg gcagagagga ccgtggtggg ggatatggaa
     4921 catatcagcg attatgtgga tatccgtccc agagactatg tgtgcgtcat gaccagaggg
     4981 caccagtttg attattacgt gcagaagcag gccatggctc tgcatcctta ttacatcgga
     5041 atcatgggaa gcaggaacaa gatacgggtg gtgacggata agctgctggc ggacggcttc
     5101 tccctggagg aaatccaaaa gtgccatatg cctatcggta cggacatagg agcagagact
     5161 ccggcggaaa ttgccatcag catagcaggg gaactgatcg cccgcagagc tgaaaggatg
     5221 ggtaaaaaga aataatgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc
     5281 cgtcgaccat catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc
     5341 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     5401 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     5461 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     5521 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     5581 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     5641 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     5701 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt
     5761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6061 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7021 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7621 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     7681 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     7741 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     7801 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7861 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7921 aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca tttacgttga
     7981 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     8041 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     8101 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     8161 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     8221 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     8281 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     8341 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     8401 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     8461 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     8521 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     8581 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     8641 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     8701 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     8761 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     8821 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     8881 taccgaagac agctcatgtt atatcccgcc gtcaaccacc atcaaacagg attttcgcct
     8941 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     9001 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     9061 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9121 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagcgcgaa ttgatctg
//
