LOCUS       pMB-pXD80-Eadh1        12068 bp    DNA     circular UNA 15-JUL-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...87-fur2xpo
VERSION     urn.local...87-fur2xpo
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(19..813)
                     /gene="aphA"
                     /locus_tag="Eggerthella_lenta_DSM11767_00914"
                     /EC_number="2.7.1.95"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P0A3Y5"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     terminator      complement(1334..1367)
     terminator      complement(1368..1409)
     CDS             complement(1497..2108)
                     /function="br"
                     /transl_table=1
                     /gene="icymR /ifromiPseudomonas putida/i"
                     /codon_start=1
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAA
                     GVSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFF
                     LDDDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDIL
                     WLIFNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR*"
                     /experiment="br"
                     /protein_id="br"
                     /product="cumate repressor (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /standard_name="CymR"
     promoter        2109..2595
     terminator      2297..2330
     terminator      complement(2332..2370)
     protein_bind    2512..2539
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the ip-cmt/i
                     operon ofiPseudomonas putida/i (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /allele="br"
                     /standard_name="CuO"
     misc_feature    2571..5704
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Gedh1 (concatenated sequence 2)"
     misc_feature    2571..2595
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2595^2596
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATGCA"
     misc_feature    2596..5679
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CCTTGGCGCTTCCCGTCTCGGTCGCGCGCAAGTGAAGATTAT
                     ACCGGAACGCAGCCTTTGCGCAAGACCGAATTTGCGTAAATTCACTTACCCTCAAATT
                     CGGCGGCAGATTCAGCACCTGCAATGCTCGACGCAACGGAACCGCGTTGGGCTGTTCG
                     TTCCAATATCGGATGAGACATGCAGCTTCGGCATCATTACGCAGCCCTGGTGGGATCT
                     CCCGCATCATCAAACACGCGAAGCGGGTACGCCGGCTCGATCGCAACTGCCCCGTGGC
                     GGCCTAGCAGCCGCCTCCTCTCCATCCCTACCCTTTTAGCACGCCCTTCGCACCGGCG
                     CCCCTTCCTGGGCTTGCTTCTTAATTCATAGCGTCTTCCCAGTTGGGAGTCTCATACC
                     AAGAGGATTACCGCCGAAAACGCTCGGGTTGGAATAATGAAAAGGGCTTCCTCATCAT
                     CTGCGGGGGACGCCGCTCCACGAATCCTCCTTATTCTGGCCCCGTTCGGAATCGTGCC
                     CGCCCACCGCGCGCGCAAGCCGTGACGGAACCGGGCCGGAGACGACCCCGATGCGAGA
                     CGAGAACCGATGAGAAGCAACGAGAAGGAGGAACGT"
     misc_feature    <2596..>2637
                     /note="Geneious type: Expression casette"
                     /standard_name="expression cassette"
     primer_bind     2596..2616
                     /standard_name="oMB-Eadh1-F"
     source          complement(<2596..>5679)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     CDS             2596..>2637
                     /locus_tag="DMP12_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="ROT90869.1"
                     /db_xref="GI:1516465669"
                     /standard_name="dehydrogenase CDS"
     misc_feature    2599..2616
                     /standard_name="His6 affinity tag"
     misc_feature    2617..2637
                     /standard_name="linker"
     source          complement(<2638..>5679)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     gene            <2638..5037
                     /locus_tag="BN3560_RS12915"
                     /db_xref="GeneID:47671105"
                     /standard_name="gene"
     CDS             <2638..5037
                     /codon_start=1
                     /locus_tag="BN3560_RS12915"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012602.1,HMM:NF013717.1,HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_161959475.1"
                     /db_xref="GeneID:47671105"
                     /translation="ADSAQAPVQEHWSETNGKKYRRGSRLPKKTDTRPPVPAAETPKV
                     PWTWEEDGMTVIRGTARSAPGCHNVCGILSYVKDGKLVKVEGDPEDPYNQGRLCSRCL
                     CIPDYVYHEDRLTKPMKRDRADRGKDKFVEISWDEAYDIIVREFKRVVDTYGADKIAM
                     CQGTGRDIHQVTRLNACVGSPNEGVPYFAGNSCYLPRIASMACMLGGACVMDCSQFLP
                     QRYDDPRYTVPEYCIVWGHQPFYSNADGFYGHWITDLMKRGMKVAVVDPQLTWLAARA
                     GEDWLRVRPGGDGALAMAMLKVVCDEKLYDQDFCDKWVYGLEAVCERVAEMDLDDLCE
                     RAWVAKEDVQRVARTYAASKPAAIQWGVALDQQTGGQQAAHAITALWCITGNLDVPGG
                     NVMGDACWGIEQPNWTGTWGWDELMTEEEQSKRIGVERYPMFAVGFKNLSSNATIEAW
                     QRGEIPIRAAYIVTNNFLATMGAQAAQQLEWYKQIDFIVVSDLFMTPTMMALADVVLP
                     AATYEERDGFGGLNAYRISCINKAIEPVGDSKPDNTIFLELGKRLTAAIKPENQDIAW
                     PWDDVQEMWDYALEDGGFTWDELRESTWKYPEFKYRKYETGDLRPDGQPGFRTETGRA
                     EIYSMVFHHTSWSGLDPLPSYVEPVESPYSAPEDVEEYPYIVTSGARVPHFFHSEQRQ
                     VKKLRALHPDPLVYLHPDTAAANGIEEGDWVWLENKHGKCKYKAAFNDTYDPRVMQAE
                     HGWWFPERKDAAEENVENEQEGLFGVLESNINNLVPFDAGVSGFGSNYKAMMCKIRKA
                     D"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     gene            5066..5410
                     /locus_tag="BN3560_RS12910"
                     /db_xref="GeneID:47671104"
                     /standard_name="gene"
     CDS             5066..5410
                     /locus_tag="BN3560_RS12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_087190620.1"
                     /db_xref="GeneID:47671104"
                     /translation="MAYQGILVDVDYCTGCEACVLACQQEHGYTEAQFGLKITKLGPL
                     HIDEAKKDYQYDFIPQFTKWCDLCAERVGKGKQPTCVQHCQAQCLDWGDVEDLAKKVD
                     REKQMIVAINEA"
                     /standard_name="oxidoreductase CDS"
     gene            5482..5679
                     /locus_tag="BN3560_RS12905"
                     /db_xref="GeneID:47671103"
                     /standard_name="gene"
     CDS             5482..5679
                     /locus_tag="BN3560_RS12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015539933.1"
                     /db_xref="GeneID:47671103"
                     /translation="MAQRFEDDFGGRIAEKLMNVDVHLTRDFSRSVTELEREQGRIDW
                     KDRCGANMKKIAEREVTPIYR"
                     /standard_name="hypothetical protein CDS"
     primer_bind     complement(5658..5679)
                     /standard_name="oMB-Eadh1-R"
     misc_feature    5680..2595
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pXD80GHcdh3-pXD68Kan2-Pct3-GuroHcdhAB(NHis
                     6) (digested by SpeI, NsiI) Fragment 2 (concatenated
                     sequence 1)"
     misc_feature    5680..5704
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     terminator      5685..5725
     terminator      5734..5779
     terminator      complement(5851..5878)
     CDS             complement(6310..7161)
                     /standard_name="repC"
     CDS             complement(7148..7987)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
                     /standard_name="RSF1010 RepA"
     CDS             complement(8498..10627)
                     /standard_name="mobA_Y25F"
     CDS             complement(8498..9469)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
                     LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
                     KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
                     GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRR
                     TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
                     GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /standard_name="RSF1010 RepB"
     oriT            10708..10795
                     /standard_name="oriT"
     CDS             10826..11110
                     /standard_name="mobC"
     terminator      complement(11694..11737)
     oriT            complement(11833..11942)
                     /note="incP origin of transfer"
                     /standard_name="incP origin of transfer oriT"
     terminator      complement(12050..8)
ORIGIN      
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatgca tcaccatcac catcacgact acgacatccc gactaccgcc
     2641 gattcagccc aggcccccgt gcaggagcac tggagcgaga ccaacgggaa gaagtaccgc
     2701 cgcggctcgc gcctgccgaa gaagaccgac acgcgcccac ccgtgcctgc tgccgagacg
     2761 ccgaaagtgc cttggacgtg ggaggaggac ggcatgaccg tcatccgcgg caccgcgcgc
     2821 agcgcgcctg gatgccacaa cgtgtgcggc atcctgtcct acgtgaagga cggcaagctg
     2881 gtgaaagtgg agggcgaccc ggaggacccc tacaaccagg gacgcctgtg cagccgctgc
     2941 ctctgcatcc ccgactacgt ctaccatgag gaccgcctga ccaaaccgat gaagcgcgac
     3001 cgcgccgacc gcggcaagga caagttcgtg gagatcagct gggacgaggc ctacgacatc
     3061 atcgtgcgcg agttcaagcg cgtcgtcgac acgtacggcg ccgacaagat cgccatgtgc
     3121 cagggcacgg gccgcgatat ccaccaggta acgcgcctga acgcctgtgt gggctccccg
     3181 aacgagggcg tgccctactt cgccggcaac tcgtgctacc tgccccgcat cgcgtcgatg
     3241 gcctgcatgc tgggcggcgc ctgcgtcatg gactgctcgc agttcctccc ccagcgctac
     3301 gacgatccgc gctacacggt gccagagtac tgcatcgtgt ggggccacca acccttctac
     3361 agcaacgcag acggcttcta cggccattgg atcaccgacc tcatgaagcg cggcatgaag
     3421 gtggccgtgg tggacccgca gctcacgtgg ctcgcggccc gcgccggcga ggactggctg
     3481 cgcgtgcgtc ccggcggcga cggcgcgctg gccatggcca tgctgaaggt cgtctgcgac
     3541 gagaagctct acgaccagga cttctgcgac aagtgggtgt acggcctgga agccgtgtgc
     3601 gagcgcgtgg cggagatgga cctggacgac ctgtgcgaga gggcctgggt ggccaaggag
     3661 gacgtgcagc gcgtggcgcg cacctacgcc gcatcgaagc ccgccgccat ccaatggggc
     3721 gtggcgctcg accagcagac gggcggccag caggcagcgc acgccatcac ggccctgtgg
     3781 tgcatcaccg gcaacctcga cgtgcccggc ggcaacgtga tgggcgacgc ctgctggggc
     3841 atcgagcagc cgaactggac cggcacctgg ggctgggacg agctcatgac cgaggaggag
     3901 cagtccaagc gcatcggcgt ggagcgctac cccatgttcg ccgtgggctt caagaacctg
     3961 tcctccaacg ccaccatcga ggcgtggcag cgcggcgaga tacccatccg cgcggcctac
     4021 atcgtgacga acaacttcct ggccaccatg ggcgcccagg ccgcccagca gctggaatgg
     4081 tacaagcaga tcgacttcat cgtggtgtcc gacctgttca tgacccccac catgatggcg
     4141 ctggccgacg tggtgctgcc cgccgccacc tacgaggagc gcgacggctt cggcgggctc
     4201 aacgcctacc gcatcagctg catcaacaag gccatcgagc ccgtgggcga ctccaagccc
     4261 gacaacacca tcttcctgga gctgggcaag cgcctgacgg cggccatcaa gcccgagaac
     4321 caggacatcg cctggccttg ggacgacgtg caggagatgt gggactacgc ccttgaggac
     4381 ggcggcttca cctgggacga gctgcgcgag tccacgtgga agtaccccga gttcaagtac
     4441 cgcaagtacg agacgggaga cctgcgcccc gacggccagc cgggcttccg caccgagacg
     4501 ggccgcgcgg agatctactc catggtgttc caccacacgt cgtggtcggg cctcgacccg
     4561 ctgccgagct acgtcgagcc ggtggagagc ccctacagcg cccccgagga cgtggaggag
     4621 tacccctaca tcgtcacgtc cggcgcgcgc gttccgcact tcttccactc cgagcagcgc
     4681 caggtcaaga agctgcgcgc cctgcacccc gacccgctcg tgtacctgca ccccgacacc
     4741 gcagccgcca acggaatcga ggagggcgac tgggtgtggc tggagaacaa gcacggcaag
     4801 tgcaagtaca aggccgcctt caacgacacc tacgacccgc gcgtcatgca ggccgagcac
     4861 ggctggtggt tccccgagcg caaggacgcg gccgaggaga acgtggagaa cgagcaagag
     4921 ggcctgttcg gcgtgctgga gtccaacatc aacaacctcg tgcccttcga cgccggcgta
     4981 tcgggcttcg gctcgaacta caaggccatg atgtgcaaga tccgcaaggc cgactagcag
     5041 ccgcgcgacg gaaaggatga gagacatggc ttaccaggga attttggtcg acgtcgacta
     5101 ctgcaccggc tgcgaggcgt gcgtgctggc ctgccagcag gaacacggct acaccgaggc
     5161 gcagttcggc ctgaagatca cgaagctggg cccgctgcac atcgacgagg ccaagaagga
     5221 ctaccagtac gacttcatcc cccagttcac caagtggtgc gacctgtgcg ccgagcgcgt
     5281 gggcaagggc aagcagccca cgtgcgtgca gcactgccag gcccagtgcc tggactgggg
     5341 agacgtggag gacctcgcga agaaggtcga ccgcgagaag cagatgatcg tcgccatcaa
     5401 cgaggcgtag atctgtcggg ccgcgggcgc cgtgccaagg cggggcgccc gcggaaaagg
     5461 gaaccaggaa ggaggacgga catggcccag agattcgagg acgatttcgg cgggaggatc
     5521 gccgagaagc tgatgaacgt cgacgttcac ctgacgcgcg acttcagccg ctcggtcacc
     5581 gagctcgaac gcgagcaggg ccgcatcgac tggaaggacc gctgcggcgc caacatgaag
     5641 aagatcgcgg agcgcgaagt tacaccgatc taccggtaat cgactcacac tggctcacct
     5701 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     5761 tccggctttt ttattattta attcctgtta taaaaaaagg atcaattttg aactctctcc
     5821 caaagttgat cccttaacga tccctgcagg agaaggccat cctgacggat ggcctttttg
     5881 aagctttcta gaatcggggg cccccggggg gattctagaa caatggaagt cctcgtctcg
     5941 ccctcgaatt agcccgctta atgagcgggc tttttttgaa ttaattctcg cgagctggca
     6001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagcg
     6061 cgaattgcaa gctggccgac gcgctgggct acgtcttgct ggcgttcggg agcagaagag
     6121 catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca gcgctcagta
     6181 ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca tggtattacc
     6241 aattagcagg aaaataagcc attgaatata aaagataaaa atgtcttgtt tacaatagag
     6301 tggggggggt cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg ccgcgaactc
     6361 ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc gctggcggcg
     6421 cttgcgcatg gtcgaaccac tggcctctga cggccagaca tagccgcaca aggtatctat
     6481 ggaagccttg ccggttttgc cggggtcgat ccagccacac agccgctggt gcagcaggcg
     6541 ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg atgcgcacat gctggccgcc
     6601 acccatgacg gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc cgtccgcctc
     6661 gtcgctggcg tactccgaca gcagccgaaa cccctgccgc ttgcggccat tctgggcgat
     6721 gatggatacc ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg ccccaccact
     6781 atcgacctct gccccgattt cctttgccag cgcccgatag ctacctttga ccaccatggc
     6841 atcagcggtg acggcctccc acttgggttc caggaacagc cggagctgcc gtccgccttc
     6901 ggtcttgggt tccgggccaa gcactaggcc attaggccca gccatggcca ccagcccttg
     6961 caggatgcgc agatcatcag cgcccagcgg ctccgggccg ctgaactcga tccgcttgcc
     7021 gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc gcttgagggc
     7081 acggaacagg ccgggggcca gacagtgcgc cgggtcgtgt cggacgtggc tgaggctgtg
     7141 cttgttctta ggcttcacca cggggcaccc ccttgctctt gcgctgcctc tccagcacgg
     7201 cgggcttgag caccccgccg tcatgccgcc tgaaccaccg atcagcgaac ggtgcgccat
     7261 agttggcctt gctcacaccg aagcggacga agaaccggcg ctggtcgtcg tccacacccc
     7321 attcctcggc ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg atgttatcga
     7381 ccagtaccga gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc atggccgcgc
     7441 ccttgctggc atggtgcagg aacacgatag agcacccggt atcggcggcg atggcctcca
     7501 tgcgaccgat gacctgggcc atggggccgc tggcgttttc ttcctcgatg tggaaccggc
     7561 gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc gcgcttgagg ccgtcgaacc
     7621 actccggggc catgatgttg ggcaggctgc cgatcagcgg ctggatcagc aggccgtcag
     7681 ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg cggtgatgaa
     7741 tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc agttcgccca
     7801 cctccagcag atccggcccg cctgcaatct gtgcggccag ttgcagggcc agcatggatt
     7861 taccggcacc accgggcgac accagcgccc cgaccgtacc ggccaccatg ttgggcaaaa
     7921 cgtagtccag cggtggcggc gctgctgcga acgcctccag aatattgata ggcttatggg
     7981 tagccattga ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg ggtcactacc
     8041 cccgccctgc gccgctctga gttcttccag gcactcgcgc agcgcctcgt attcgtcgtc
     8101 ggtcagccag aacttgcgct gacgcatccc tttggccttc atgcgctcgg catatcgcgc
     8161 ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct tttggtcttt
     8221 catatcagtc accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg aacaaggaca
     8281 aggtgcagcc gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg ccagcctcgg
     8341 ccttgtttga cgtataacca aagccaccgg gcaaccaata gcccttgtca cttttgatca
     8401 ggtagaccga ccctgaagcg cttttttcgt attccataaa acccccttct gtgcgtgagt
     8461 actcatagta taacaggcgt gagtaccaac gcaagcacta catgctgaaa tctggcccgc
     8521 ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc cagctcggcc cgcgcaagct
     8581 ggacgctggg cagacccatg accttgctga cggtgcgctc gatgtaatcc gcttcgtggc
     8641 cgggcttgcg ctctgccagc gctgggctgg cctcggccat ggccttgccg atttcctcgg
     8701 cactgcggcc ccggctggcc agcttctgcg cggcgataaa gtcgcacttg ctgaggtcat
     8761 caccgaagcg cttgaccagc ccggccatct cgctgcggta ctcgtccagc gccgtgcgcc
     8821 ggtggcggct aagctgccgc tcggacagtt cgaggctggc cagcctgcgg gccttctcct
     8881 gctgccgctg ggcctgctcg atctgctggc cagcctgctg caccagcgcc gggccagcgg
     8941 tggcggtctt gcccttggat tcacgcagca gcacccacgg ctgataaccg gcgcgggtgg
     9001 tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg ctgtcggcgc
     9061 tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc cgaagttcac
     9121 cgcctgcggc gtcggccacc ttgacccatg cctgatagtt cttcgggctg gtttccacta
     9181 ccagggcagg ctcccggccc tcggctttca tgtcatccag gtcaaactcg ctgaggtcgt
     9241 ccaccagcac cagaccatgc cgctcctgct cggcgggcct gatatacacg tcattgccct
     9301 gggcattcat ccgcttgagc catggcgtgt tctggagcac ttcggcggct gaccattccc
     9361 ggttcatcat ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc tcacagccca
     9421 tggccttgag ctgtcggcct atggcctgca aagtcctgtc gttcttcatc gggccaccaa
     9481 gcgcagccag atcgagccgt cctcggttgt cagtggcgtc aggtcgagca agagcaacga
     9541 tgcgatcagc agcaccaccg taggcatcat ggaagccagc atcacggtta gccatagctt
     9601 ccagtgccac ccccgcgacg cgctccgggc gctctgcgcg gcgctgctca cctcggcggc
     9661 tacctcccgc aactctttgg ccagctccac ccatgccgcc cctgtctggc gctgggcttt
     9721 cagccactcc gccgcctgcg cctcgctggc ctgctgggtc tggctcatga cctgccgggc
     9781 ttcgtcggcc agtgtcgcca tgctctgggc cagcggttcg atctgctccg ctaactcgtt
     9841 gatgcctctg gatttcttca ctctgtcgat tgcgttcatg gtctattgcc tcccggtatt
     9901 cctgtaagtc gatgatctgg gcgttggcgg tgtcgatgtt cagggccacg tctgcccggt
     9961 cggtgcggat gccccggcct tccatctcca ccacgttcgg ccccaggtga acaccgggca
    10021 ggcgctcgat gccctgcgcc tcaagtgttc tgtggtcaat gcgggcgtcg tggcctgccc
    10081 gctctaatgc ccggttggca tggtcggccc atgcctcgcg ggtctgctca agccatgcct
    10141 tgggcttgag cgcttcggtc ttctgtgccc cgcccttctc cggggtcttg ccgttgtacc
    10201 gcttgaacca ctgagcggcg ggccgctcga tgccgtcatt gatccgctcg gagatcatca
    10261 ggtggcagtg cgggttctcg ccgccaccgg catggatggc cagcgtatac ggcaggcgct
    10321 cggcaccggt caggtgctgg gcgaactcgg acgccagcgc cttctgctgg tcgagggtca
    10381 gctcgaccgg cagggcaaat tcgacctcct tgaacagccg cccattggcg cgttcataca
    10441 ggtcggcagc atcccagtag tcggcgggcc gctcgacgaa ctccggcatg tgcccggatt
    10501 cggcgtgcaa gacttcatcc atgtcgcggg catacttgcc ttcgcgctgg atgaagtcgg
    10561 ccttggccct ggccgattgg ccgcccgacc tgctgccggt tttcgccgta aggtgataaa
    10621 tcgccatgct gcctcgctgt tgcttttgct tttcggctcc atgcaatggc cctcggagag
    10681 cgcaccgccc gaagggtggc cgttaggcca gtttctcgaa gagaaaccgg taagtgcgcc
    10741 ctcccctaca aagtagggtc gggattgccg ccgctgtgcc tccatgatag cctacgagac
    10801 agcacattaa caatggggtg tcaagatggt taaggggagc aacaaggcgg cggatcggct
    10861 ggccaagctc gaagaacaac gagcgcgaat caatgccgaa attcagcggg tgcgggcaag
    10921 ggaacagcag caagagcgca agaacgaaac aaggcgcaag gtgctggtgg gggccatgat
    10981 tttggccaag gtgaacagca gcgagtggcc ggaggatcgg ctcatggcgg caatggatgc
    11041 gtaccttgaa cgcgaccacg accgcgcctt gttcggtctg ccgccacgcc agaaggatga
    11101 gccgggctga atgatcgacc gagacaggcc ctgcggggct gcacacgcgc ccccaccctt
    11161 cgggtagggg gaaaggccgc taaagcggct aaaagcgctc cagcgtattt ctgcggggtt
    11221 tggtgtgggg tttagcgggc tttgcccgcc tttccccctg ccgcgcagcg gtggggcggt
    11281 gtgtagccta gcgcagcgaa tagaccagct atccggcctc tggccgggca tattgggcaa
    11341 gggcagcagc gccccacaag ggcgctgata accgcgccta gtggattatt cttagataat
    11401 catggatgga tttttccaac accccgccag cccccgcccc tgctgggttt gcaggtttgg
    11461 gggcgtgaca gttattgcag gggttcgtga cagttattgc aggggggcgt gacagttatt
    11521 gcaggggttc gtgacagtta gtacgggagt gacgggcact ggctggcaat gtctagcaac
    11581 ggcaggcatt tcggctgagg gtaaaagaac tttccgctaa gcgatagact gtatgtaaac
    11641 acagtattgc aaggacgcgg aacatgcctc atgtggcggc cctcgcgaga attaattcag
    11701 ataaaaaaaa tccttagctt tcgctaagga tgatttctag cgatgaccct gctgattggt
    11761 tcgctgaccg tagactggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
    11821 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
    11881 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
    11941 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
    12001 tatatcgtgc gacatcgcgc gcgcgcgcgc ggatgctagc ggcgcgccaa aaaaaagccc
    12061 gctcatta
//
