LOCUS       pMB-pXD80-Eadh2        12415 bp    DNA     circular UNA 15-JUL-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...93-fur3a9a
VERSION     urn.local...93-fur3a9a
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(19..813)
                     /gene="aphA"
                     /locus_tag="Eggerthella_lenta_DSM11767_00914"
                     /EC_number="2.7.1.95"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P0A3Y5"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     terminator      complement(1334..1367)
     terminator      complement(1368..1409)
     CDS             complement(1497..2108)
                     /function="br"
                     /transl_table=1
                     /gene="icymR /ifromiPseudomonas putida/i"
                     /codon_start=1
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAA
                     GVSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFF
                     LDDDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDIL
                     WLIFNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR*"
                     /experiment="br"
                     /protein_id="br"
                     /product="cumate repressor (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /standard_name="CymR"
     promoter        2109..2595
     terminator      2297..2330
     terminator      complement(2332..2370)
     protein_bind    2512..2539
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the ip-cmt/i
                     operon ofiPseudomonas putida/i (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /allele="br"
                     /standard_name="CuO"
     misc_feature    2571..6051
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Gedh2 (concatenated sequence 2)"
     misc_feature    2571..2595
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2595^2596
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GCCTTCGGAGCCGCAGGAGGGGTGCGAAGGGCACCCCTCCTG
                     CGCATGGCCGTAGGCCGCGCCGAAGAACATCGGCCACCGCCAAGCAAGACGAGGTAGA
                     GAAGGAAACCATGTACGAGAAGCGCATGAACGACCAACCGGGGGAACAGCCCGACGCC
                     GTCACGGGCGCCACGAGCCTGGCCGATGCGGCGGCCGCCCTCGACCCCGGCCAGCACG
                     GAGGCGCTCCGGCGGCGCCTCCCCCCTGCCCGCCTCCGGGCACCTGCGATGCCGACGC
                     CGTCACCGGCGCCACCCCCGGCGTTGCCAGCGCATGCCGCGAGCTGGGCATGTCGTCG
                     GTGGACAGCGTCGTTCGCGAACGCGGGATCAAGCCGCCGGGGGTAGCCTGAGGGCGGC
                     GGACGGCTCCCTGTGCCGACGGGCACGCATGCGCATCTGATGGAGCTTGCCCTGCTTC
                     CGACCGTCGGCAAACTATAGGGAGCGGCTCTCCGCCTGCGCCGGAACGGCCCTCTCGC
                     TCTCCTTTTGATGTATACTGAGGTTCCGGAGATGTTGGGGTCCGCGTCCTGAATCGGT
                     GTCCGGCAGGCCGCGCCGATCGTGCTCAGTTTCTCCAACGACGTTCAAGCCGGGCACT
                     GCGGGGGGAATCAGATGGACGACGGCAAGACCGTGGACATGCGCCGCATCGGCGCCAT
                     GGAGCTGCTGGGGATCGGCGGGTTCTCCTGCTTTTTCGGGTGGATGCTCGTATCGTAC
                     TATTGGCTTTTCGCCGAGTTCATCCAGGACACCCCCGTAGCCGAGCGCGACGCCATCC
                     AGTTATTCGTTTTCGCAGGTCTGGCACTGGGATATTTGTCCCTGCACCTTCTTGCCAA
                     GAACTCCCGGTTCGATCCGTTCAGGCTGTCCGCGCTTGCGACCGATGCTGCGCTGGCG
                     CTGCTCTTGCCCATCGCCGCCCTGTTTCTGGGCCTCGGCGTGGACGTACCCTCCCCAG
                     TGCTGTGCGTCGTGAACCTTTGCGCGGGCCTGGGCGGCGCATCGCTGACGGTGAGCTG
                     GCTCGATGCGAGCAGCCGTATCAGGGTGAAGAGCTTTCCTCGCTTCACGAGCCTGTCG
                     CTCGTGGGCGGCGGGGCGCTGTTCGTCCTCGCCGCCATCGTTCCCGAGGCCGTACAGC
                     CCGTGTTCTGCATCGTCTATGCGCTGCTTAGCGTCGGCCTCCTCCGTTTCGCCGGCGA
                     GCGAGCCGAAGGCAACGCCGATGCGCCCCCCGCCGGCTCGTGCAAGAACACCTGGGAG
                     TTCACCTGGGAGATAGAACCCTCGCTCGTGGCGTTCGGCATCGTGTTCGGCCTGACGT
                     TCGTGTACCTGTTCAACTCAGGCCAGCAAGACGTGCTCGTGGGCCTTCTGTTCGTGAT
                     ACCCGGTGCCGGCTTGATCGCCGTGCTCGCGCTTTCGGGCAAAGCCGTGGGCATCACC
                     GTCATCCAGCGCGTGTTGCTGTGCATCACCGTGCTGGCATGCCTCTCCATGCCCTTCG
                     CCACCGAGGCCATCCAGCTGGCATGCTCGTGCCTGGTCGTGGCGTCGTGGGCGGCGTT
                     CGTATCGGTGAACTACGCGTTCATCGTGAAGAAGAGCGTCGAACGCTGGGACACGCCG
                     GTGTTCCGCCAGGCGCCAGCGCGCCTCGTGTTCAGCGCGCTGGGCTTCCTCGTGGGCT
                     GGGGCGTGGCCACGGGCATGACCATGGCCTACGGTGCCCATTCCGACGCGTTCATGAT
                     CGTGCGCCTATGCATGGCGTTCGTGCTCGTTGCGGTGGTCATGCTGTTCTTCCCCTCG
                     AAAGAGCACCATGCGCCAGACGGCGGGACGATCGGCGCCCGCGAACCCGCCGCTCCCG
                     TGCCGTCCAACCTTAGCGAGAAGGAGCTCTTCGAGCGCCGCTGCGCCGCCGTGGCCGA
                     GCTCTACCAGCTCTCCCCGCGCGAGACCGATATCTTGAAGTTCCTGGCGAAGGGGCGC
                     AACGCCGCCTACATCCAGGGCAAGCTCACCATATCGCCCCACACGGTGAAGAGCCACA
                     TCTACAGCATCTACCGCAAGCTCGACATCCACTCCCAGCAGAAGCTCATGGACTTCGT
                     GGAGGAGTTCCCGGTGGAGGACTAGCGGGACGGCGCGGGAAAGCGGCAATGCGCCGGA
                     AGGCTAGAGTCCCTCGTAATGGGTCCACATGCGCACGACTTTGACCGTGCCATCGAAT
                     TCCTCGCCACCCTCGGCAAAGGGCTCTGCATATACCTGATAAACGAGCCGATGCTGCA
                     GGTTGATGCGCCGCGAATAGAGGCCCGAGAGGTTCCCCACAAGCGTTTCGTAGGCAGG
                     AGCCTCTTGAAAGGGATCATTCTTAAGCACCTCGACAAGTGCTTTCGCCTTCTTGTCG
                     AGACCAGCGGCTTTAAGCTTCTTTGCATCCTTTGCAGCCTGCTTCGTGAACTTCACTG
                     CATACATAACTACCACTCAAGTCGGTCTTCAGAAAGGCAGTCCTCGGGTTTTTCCGCC
                     ATGCCGGCCCGCAGCGATTCGCTCATGCCGGGAATGCCGTTGAGGAAAAGCGTTTCCT
                     CGATAGCCGCCCAATCGGATTCGCCGATAAGCACTGCCCCCTTCCCCTTGCTGTTCGT
                     GATGGCGATGGGCGTGCAGTTCTCGTTCACCTGGGCGATGGTCTGGTAGATATTCTGC
                     CGCAAAGCCGTTGCAGTGATCGAAGTCATGGCCTGTCTCCTTACCTCGTTTCTTTTGC
                     TCTATAGTAGCGTACGCAATTACGTACGTCAAGGAACCTCGTCTTCATACCAAGGAGC
                     CTCGCCTACCCCTTTTCCATCGTCCCTGTTCATCGCGTCATACCAACGTACTGATTCC
                     CAATCTGCATACTCATCACGCGATAGGCTCATACCTATCGCGTGCTTTACTCGCTGGC
                     AACCCCAGAGGGGAGGGCCGATTGGGGCGGACACCCGACAGGCCCGGAGACCGAGAGG
                     AGTTGCG"
     misc_feature    2595^2596
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATGCA"
     primer_bind     2596..2616
                     /standard_name="oMB-Eadh2-F"
     source          complement(<2596..>6026)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     gene            2596..5013
                     /locus_tag="BN3560_RS12865"
                     /db_xref="GeneID:47671095"
                     /standard_name="gene"
     CDS             2596..5013
                     /locus_tag="BN3560_RS12865"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012602.1,HMM:NF013717.1,HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_161959474.1"
                     /db_xref="GeneID:47671095"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     misc_feature    2599..2637
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <2599..>2637
                     /note="Geneious type: Expression casette"
                     /standard_name="expression cassette"
     misc_feature    2599..2616
                     /standard_name="His6 affinity tag"
     source          complement(<2599..>2637)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     CDS             <2599..>2637
                     /codon_start=1
                     /locus_tag="DMP12_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="ROT90869.1"
                     /db_xref="GI:1516465669"
                     /standard_name="dehydrogenase CDS"
     misc_feature    2617..2637
                     /standard_name="linker"
     gene            5013..5372
                     /locus_tag="BN3560_RS12860"
                     /db_xref="GeneID:47671094"
                     /standard_name="gene"
     CDS             5013..5372
                     /locus_tag="BN3560_RS12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_096228358.1"
                     /db_xref="GeneID:47671094"
                     /translation="MALNGILVDYQYCTGCYSCEVACQAEHELPLEQWGVKVMQNGPW
                     PVKDAEGNETDNYVYDFIPAFTKICDLCADRQEKGKLPSCVFHCQAKCMEFGPVDELA
                     KRLDAKPQQYLWVPPCA"
                     /standard_name="oxidoreductase CDS"
     gene            5484..6026
                     /locus_tag="BN3560_RS12855"
                     /db_xref="GeneID:47671093"
                     /standard_name="gene"
     CDS             5484..6026
                     /locus_tag="BN3560_RS12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_096228357.1"
                     /db_xref="GeneID:47671093"
                     /translation="MDYQKLEEGIEKAPLASRPALERLLLYVSAGPDVSPDYAPYLEG
                     SASYQDFFNAIYADDAQKGTSVWAEWAALKRKSWIGRFEPVLAVENLRLKGDGLPVQF
                     GTGLFLAPTGSRDSIANLYVFERGAFNVEAAEFVTSIGGTFSCAGYDFAGIYGVYKYR
                     GSVILEQWEAERDPVPAKKG"
                     /standard_name="hypothetical protein CDS"
     primer_bind     complement(6008..6026)
                     /standard_name="oMB-Eadh2-R"
     misc_feature    6027..2595
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pXD80GHcdh3-pXD68Kan2-Pct3-GuroHcdhAB(NHis
                     6) (digested by SpeI, NsiI) Fragment 2 (concatenated
                     sequence 1)"
     misc_feature    6027..6051
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     terminator      6032..6072
     terminator      6081..6126
     terminator      complement(6198..6225)
     CDS             complement(6657..7508)
                     /standard_name="repC"
     CDS             complement(7495..8334)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
                     /standard_name="RSF1010 RepA"
     CDS             complement(8845..10974)
                     /standard_name="mobA_Y25F"
     CDS             complement(8845..9816)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
                     LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
                     KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
                     GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRR
                     TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
                     GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /standard_name="RSF1010 RepB"
     oriT            11055..11142
                     /standard_name="oriT"
     CDS             11173..11457
                     /standard_name="mobC"
     terminator      complement(12041..12084)
     oriT            complement(12180..12289)
                     /note="incP origin of transfer"
                     /standard_name="incP origin of transfer oriT"
     terminator      complement(12397..8)
ORIGIN      
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatgca tcaccatcac catcacgact acgacatccc gactaccgta
     2641 gagaacgctg tcgcggagaa ggaccgcgac gagatcggca cgtccaagga ggagaaccag
     2701 ttcaacttcg gcgccggcaa gaggcaggaa gtggatcctc gctggaagca tccgaagccc
     2761 ttccagtacg aggaggacgg catggtggtc acccgcacct cggtgtggag cgcgccgggc
     2821 tgccacgagg gctgtggcgt gctggtgtac tccgacaagg agaccggcaa gttcatcaag
     2881 tgcgagggcg acccggacga cccgtacaac cgcggcgcgc tgtgcccgag gtgcctggcc
     2941 ttcaagcagg tggagttcca ccccgaccgc atcctgcacc ccatgaagcg tgccggcgag
     3001 cgcgggagcg gccagtggga gcgcatcacg tgggacgagg cgctggaaac ctgctacaag
     3061 gaattccgtc gcatctccat gacctacggc ggcgactcca tccactgcct gcgcggcacc
     3121 gcacgcgaca accagtggca ggtcggccgc atggcgaaca cgttcggcag ccccaacgag
     3181 tacggcttcc tgtccggcac cgcctgctac ctgccccgcc tgtcgctcat gatcatgacc
     3241 tacggcggca tgctcatcgc cgacttctcg cagttctcgg cgctgcgcta cgacgacccc
     3301 gagtgggtgt gccccgagtg caccatcgtt tggggctgca accccaccat ctccaacccc
     3361 gacttcttca tgggacactg ggtcaccgac gccatgaagc tcggctgcaa gctcatctcc
     3421 gtcgagccgc gcgtcacctg gctcgccgcg cacgccgaca tccatctgca gctgcgcccc
     3481 ggcaccgaca cgggcctcgc tctcggcatg atcaaggtca tcatcgacga ggacctctac
     3541 gaccatgact tcgtggagcg ctggacctac ggcttcgacg agctggccga gcgcgcccgc
     3601 gagctgtcgc tggaccaggt ggaggccatg acgtgggtcc ccaaggagaa gatcatcgcc
     3661 gctgcgcgcc tgttcgccac gtccaagccg gccaacgtcg tgtggggcct cgccgtggac
     3721 atgcagagcc agggcacgcc ctgcgccgcc gccatcgccg cgctgtggac catcaccggc
     3781 aacctcgacg tgccgggcgg catgtgctac accgcctccc ccatgggcgt cgaccagccg
     3841 tccgccggcg catggggcat ctacgacctc atcaacgagg agatgcagaa gaagcgcgtg
     3901 ggctggaaag aattccccat gtaccgctac ggcctcaccc aggccatgcc cgacatgtgc
     3961 ctggagtata tggaggaggg caaggtcaag ggcgtgtgga tccagacatc caacggcatt
     4021 gcctgcatga gctgcgagac cgagcgctgg taccaggcca tgaagaagcc ggagttcatc
     4081 gccgccgtgg acatcttcat gacgcctacc atccagtcgt gcgccgacat cgtcatgccg
     4141 gtgcagacgt gggccgagaa gcactccgta cgcgcccact actacttcct gtccgctatc
     4201 acgggcggct gcgcggccga gggcgaggcg aagtcggact gcgagatcaa ccgcgagctt
     4261 gcccagtact tcgacaacga cgacgagttc aaccaggcca tcggccgccc cgagggcaag
     4321 cagcacacct ggccgtgggc gaccgaggac gaggtgtacg acgagatcgt gagcccctcc
     4381 ggcttcacgt tccacgagct catggagcac ggcccggtct accagaagta cgtgtacaag
     4441 aagtacgaga agggcctcat gcgccccgac ggccagcccg gcttcaacac ccccacgggc
     4501 cgcatcgagt tctactccac gctgttcgag aagttcggct acgacccgct gccctacatc
     4561 gaggagccgg gcatcgggcc cgtcaccacg cccgacctgt acgaggagta cccgctcatc
     4621 atgatcacgg gcgcccgcac cacgtcgttc ttccactcgg agcaccgcca gatcccctac
     4681 ctgcgccagc tcacacccga tccgtgggtg cagatccacc cgcgcacggc caaggacctg
     4741 aacatctccg agggcgactg ggtctggatc gagaaccacc gcggccgctg ccgccagcgt
     4801 gcccgcctca cgttcgaggt gcacgagaag gagatcgccg cgcagcacgg ctggtggttc
     4861 cccgaacagg acggcgccga acccaacctg tacggcttcc gccagtccaa catcaaccag
     4921 cttctggcca acaagcccgg cacgactggc ttcggtgccg acctgaagtg cacgctctgc
     4981 aaggtgtacc gctgcaagga ggaggaactg taatggctct caacggaatt ctcgtcgact
     5041 accagtactg cacggggtgc tactcgtgcg aggtggcctg ccaggccgag cacgagctac
     5101 cgctcgagca gtggggcgtg aaggtcatgc agaacggccc gtggccggtg aaggacgccg
     5161 aaggcaacga gacggacaac tacgtctacg acttcatccc ggcgttcacg aagatctgcg
     5221 atttgtgcgc cgaccgccag gagaagggca agctcccctc ctgcgtgttc cactgccagg
     5281 ccaagtgcat ggagttcggc ccggtggacg agctcgcgaa gcgcttggac gccaagccgc
     5341 agcagtacct gtgggtgccg ccctgcgcgt agcgccttga gctgggacgg ccgccgagcc
     5401 gtcccagctc tccccaagat gccggcagcc ccgagcgacg gccggcatct tggggagaac
     5461 ctcggttgaa aaggagaccg gctgtggact accaaaagct tgaagagggc atcgagaagg
     5521 caccgctcgc ctcgcgcccc gcactggaac gcctgctgct gtacgtgtcg gcaggcccgg
     5581 acgtatcgcc cgactacgcg ccgtacctgg aaggctcggc atcctaccag gactttttca
     5641 acgccatcta cgccgacgac gcccagaagg gcacgtcggt atgggcggag tgggcggcgc
     5701 tcaaacgcaa gagctggatc ggccgcttcg aacccgtcct ggccgtggag aacctgcgcc
     5761 tcaagggcga cgggctgccg gtgcagttcg gcacgggcct gttcctggcc cctaccggca
     5821 gccgcgacag catcgcgaac ctctacgtgt tcgaacgcgg cgcgttcaac gtggaggcgg
     5881 ccgagttcgt cacgtcgatc ggcggcacgt tctcctgcgc cggctacgac ttcgccggca
     5941 tctacggcgt gtacaagtac cgcggcagcg tgatactcga gcaatgggag gccgagcggg
     6001 accccgtgcc agcgaagaag ggctagtcga ctcacactgg ctcaccttcg ggtgggcctt
     6061 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     6121 ttatttaatt cctgttataa aaaaaggatc aattttgaac tctctcccaa agttgatccc
     6181 ttaacgatcc ctgcaggaga aggccatcct gacggatggc ctttttgaag ctttctagaa
     6241 tcgggggccc ccggggggat tctagaacaa tggaagtcct cgtctcgccc tcgaattagc
     6301 ccgcttaatg agcgggcttt ttttgaatta attctcgcga gctggcacga caggtttccc
     6361 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct
     6421 ggccgacgcg ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa
     6481 gcaaagccag gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa
     6541 aatattgtta agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa
     6601 ataagccatt gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag
     6661 cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag
     6721 cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc
     6781 gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg
     6841 gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg
     6901 tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc
     6961 tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac
     7021 tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc
     7081 caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc
     7141 ccgatttcct ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg
     7201 gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc
     7261 gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga
     7321 tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca
     7381 tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg
     7441 ggggccagac agtgcgccgg gtcgtgtcgg acgtggctga ggctgtgctt gttcttaggc
     7501 ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac
     7561 cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct
     7621 cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc
     7681 ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct
     7741 gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg
     7801 gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac
     7861 ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag
     7921 caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat
     7981 gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg
     8041 ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg
     8101 gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc
     8161 cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc
     8221 gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg
     8281 tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg
     8341 cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc
     8401 gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac
     8461 ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg
     8521 tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc
     8581 gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc
     8641 aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt
     8701 ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc
     8761 tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa
     8821 caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc
     8881 tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag
     8941 acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc
     9001 tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg
     9061 gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt
     9121 gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag
     9181 ctgccgctcg gacagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc
     9241 ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc
     9301 cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt
     9361 gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc
     9421 gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc
     9481 ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc
     9541 ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag
     9601 accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg
     9661 cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg
     9721 gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg
     9781 tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc
     9841 gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc
     9901 accaccgtag gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc
     9961 cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac
    10021 tctttggcca gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc
    10081 gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt
    10141 gtcgccatgc tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat
    10201 ttcttcactc tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat
    10261 gatctgggcg ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc
    10321 ccggccttcc atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc
    10381 ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg cctgcccgct ctaatgcccg
    10441 gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc
    10501 ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg
    10561 agcggcgggc cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg
    10621 gttctcgccg ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag
    10681 gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag
    10741 ggcaaattcg acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc
    10801 ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac
    10861 ttcatccatg tcgcgggcat acttgccttc gcgctggatg aagtcggcct tggccctggc
    10921 cgattggccg cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc
    10981 tcgctgttgc ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa
    11041 gggtggccgt taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag
    11101 tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa
    11161 tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa
    11221 gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa
    11281 gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg
    11341 aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc
    11401 gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg
    11461 atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa
    11521 aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt
    11581 agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg
    11641 cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc
    11701 ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt
    11761 ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt
    11821 attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg
    11881 acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg
    11941 gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag
    12001 gacgcggaac atgcctcatg tggcggccct cgcgagaatt aattcagata aaaaaaatcc
    12061 ttagctttcg ctaaggatga tttctagcga tgaccctgct gattggttcg ctgaccgtag
    12121 actggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
    12181 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
    12241 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
    12301 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgac
    12361 atcgcgcgcg cgcgcgcgga tgctagcggc gcgccaaaaa aaagcccgct catta
//
