LOCUS       pMB-pXD80-Eadh3        12422 bp    DNA     circular UNA 15-JUL-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...44-fwzv4kr
VERSION     urn.local...44-fwzv4kr
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(19..813)
                     /gene="aphA"
                     /locus_tag="Eggerthella_lenta_DSM11767_00914"
                     /EC_number="2.7.1.95"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P0A3Y5"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     terminator      complement(1334..1367)
     terminator      complement(1368..1409)
     CDS             complement(1497..2108)
                     /function="br"
                     /transl_table=1
                     /gene="icymR /ifromiPseudomonas putida/i"
                     /codon_start=1
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAA
                     GVSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFF
                     LDDDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDIL
                     WLIFNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR*"
                     /experiment="br"
                     /protein_id="br"
                     /product="cumate repressor (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /standard_name="CymR"
     promoter        2109..2595
     terminator      2297..2330
     terminator      complement(2332..2370)
     protein_bind    2512..2539
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the ip-cmt/i
                     operon ofiPseudomonas putida/i (a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""Mull
                     ick et al., 2006/a)"
                     /allele="br"
                     /standard_name="CuO"
     misc_feature    2571..6058
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Gedh3ABC (concatenated sequence 2)"
     misc_feature    2571..2595
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2595^2596
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATGCA"
     primer_bind     2596..2616
                     /standard_name="oMB-Eadh3-F"
     source          complement(<2596..>6033)
                     /organism="Ellagibacter isourolithinifaciens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="DSM104140T_contig_001"
                     /strain="DSM 104140"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="DSM:104140"
                     /type_material="type strain of Ellagibacter
                     isourolithinifaciens"
                     /db_xref="taxon:2137581"
                     /country="Spain: Murcia"
                     /collection_date="2016-06-15"
     gene            2596..4965
                     /locus_tag="F8C90_RS00245"
                     /old_locus_tag="F8C90_00245"
                     /standard_name="gene"
     CDS             2596..4965
                     /locus_tag="F8C90_RS00245"
                     /old_locus_tag="F8C90_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_158048609.1"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     misc_feature    <2599..>2637
                     /note="Geneious type: Expression casette"
                     /standard_name="expression cassette"
     misc_feature    2599..2616
                     /standard_name="His6 affinity tag"
     source          complement(<2599..>2637)
                     /organism="Gordonibacter urolithinfaciens"
                     /mol_type="genomic DNA"
                     /strain="DSM 27213T"
                     /type_material="type strain of Gordonibacter
                     urolithinfaciens"
                     /db_xref="taxon:1335613"
                     /chromosome="I"
     gene            <2599..>2637
                     /locus_tag="BN3560_RS12865"
                     /db_xref="GeneID:47671095"
                     /standard_name="gene"
     misc_feature    2617..2637
                     /standard_name="linker"
     gene            4968..5333
                     /locus_tag="F8C90_RS00240"
                     /old_locus_tag="F8C90_00240"
                     /standard_name="gene"
     CDS             4968..5333
                     /locus_tag="F8C90_RS00240"
                     /old_locus_tag="F8C90_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="WP_158048442.1"
                     /translation="MAEKAYGLLINYEFCTGCGTCEMACSVEHDIPIGRYGIQMATIG
                     PWQIDKKNWQLDTVPIPTDECDLCAKRVEKGKKPTCVKHCQSLCMEYGPVDELTAKLA
                     GATTKHVLFVPKAGTAAKQ"
                     /standard_name="4Fe-4S binding protein CDS"
     gene            5680..6033
                     /locus_tag="F8C90_RS00235"
                     /old_locus_tag="F8C90_00235"
                     /standard_name="gene"
     CDS             5680..6033
                     /locus_tag="F8C90_RS00235"
                     /old_locus_tag="F8C90_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158048441.1"
                     /translation="MIAMEDVKPIEVEASIPAEQAAAPSNSLKRKRPEGEGAVEIYCT
                     KCGESATDGRFSPYGPSMEWWYTIAGTLDEIQWVYQTDKNYWAKVVEPTVRCGCGSGR
                     ECNPMLWSVRQIPAS"
                     /standard_name="hypothetical protein CDS"
     primer_bind     complement(6014..6033)
                     /standard_name="oMB-Eadh3-R"
     misc_feature    6034..2595
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pXD80GHcdh3-pXD68Kan2-Pct3-GuroHcdhAB(NHis
                     6) (digested by SpeI, NsiI) Fragment 2 (concatenated
                     sequence 1)"
     misc_feature    6034..6058
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     terminator      6039..6079
     terminator      6088..6133
     terminator      complement(6205..6232)
     CDS             complement(6664..7515)
                     /standard_name="repC"
     CDS             complement(7502..8341)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
                     /standard_name="RSF1010 RepA"
     CDS             complement(8852..10981)
                     /standard_name="mobA_Y25F"
     CDS             complement(8852..9823)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar
                     Typhimurium"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
                     LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
                     KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
                     GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRR
                     TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
                     GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /standard_name="RSF1010 RepB"
     oriT            11062..11149
                     /standard_name="oriT"
     CDS             11180..11464
                     /standard_name="mobC"
     terminator      complement(12048..12091)
     oriT            complement(12187..12296)
                     /note="incP origin of transfer"
                     /standard_name="incP origin of transfer oriT"
     terminator      complement(12404..8)
ORIGIN      
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatgca tcaccatcac catcacgact acgacatccc gactacctac
     2641 accggcggag acgtgccttg gcagtgggaa gaggacggca tgatctgcac caggtcggcc
     2701 gcctggtctg cgccgggctg ccatgacggc tgcggcgtca tcgtgtatac ggacaaggag
     2761 accggcaagt tcatcaaggt cgaaggcgac gaggagaatc cgtactacca ggggcgcctc
     2821 tgcatgcgct gcctcgacgt ggccgaggcg atctaccatc ccgaccggct gctccacccg
     2881 atgaagcgcg acccgaagtt ccgcggcaag gcggacaagt gggagcggat cacatgggaa
     2941 gaagcctacg acatgatcga cgagaagttc accaagattc gcgacgagta cggcggaaag
     3001 gcgatcatct tcgcatccgg caccggccgc gtcacaccgc cgattaacgc acgcctggga
     3061 tatgcgctcg gctcgatcca gtactcgtat ttccattccg gcaacgcgtg ctacgttccc
     3121 cgcgttgcag ccgcgaacac catccagggc tgctacacct gccccgactt ctcgatgcag
     3181 ttccccgacc gctacgacaa tcccgaatgg gtcgccccga agaacatctt cgtctggggc
     3241 aacaacccgc ttatcgccaa tgctgacggc aacctcggcc attgggtcgt cgactgcatg
     3301 aagcgcggct cgaagctcat cgtcgtcgac ccgcgtttga cctggctcgc tgcgaaggca
     3361 gacctctggc tgcagattcg ccccggcacc gactccatgc tcgcactgtc catgggcaaa
     3421 tacatcatgg aaaacggcct ctatgaccac gagttcgtcg agaaatggtg ctacggcttc
     3481 gaggagtacg agaaggcttg cgagccctac aacctcgact gggcctctga ggtcacctgg
     3541 cttgacaagg aagacatcat cgccgctgcg aagtacatgt ccgagaagcc gacggccgtg
     3601 cagtggggcc tggccatcga catgaacctg cagtgcgtca ccgcttccca ggcactgtgc
     3661 aacctgtggt gcatcaccgg ccagatcgac atccccggcg gcatgatcac cgtgcacgac
     3721 ccgtacaaca ccgaggtttg gctgccgccc gatccccgcg aggtcttcac ccctgagcag
     3781 gagaaagagc gcatcggctc caactacgag atgatcacca actccggcat ggtccagtgc
     3841 caggcggact ccatgatcga tcagctggtc accggccgtc cgttcaagat ccgcgcagca
     3901 tggatccagt cgaccaaccc gctcgcctgc tgcgcacagg accccgagcc ccgcgtcgag
     3961 aagggtctta ggaactgcga gttcgtcgtc ggcgtcgact gcttcatgac gccaaccctc
     4021 atggcaaact gcgatgtcgt gctgccgctg tgcttttacc ctgagcgcga gggcctgcgc
     4081 tcggtgtggt actacatcca aaacaccaac aaggcctgcg agccgatggg cgagtcgaaa
     4141 gacgacttcc agatcaactg ggagctcggc cgccgctgga acaaggacct ctggccaggt
     4201 gaaacgcttg aggagtactt ctcctacctc atcaaggagg ccggcatcac ctacgacaag
     4261 tcccgcaaac tcaactggat ctaccccgag ttccactatc gcaagttcga gaagggcgag
     4321 cagcgccccg acggcaaggt gggattcaac acaccgaccg gccgcatcga gctgtggtcg
     4381 acgctgctgg catcctgggg gcagaagccg ctgccctact tcgaggagcc gccgatgagc
     4441 ccctacagcc agccggaact ctacaaggag tacccattgg tcctcaccac cggcgcacgc
     4501 cactacgagt ccttccactc tgagcacagg cagatgagcc gcctgcgctc cagacttccc
     4561 cagccgctgt tcgaaatcca tcccgacaaa gcggcagagc tcggcatcga agacggcgac
     4621 tggtgctgga tcgaaagccc catcggccgt tgccgcgagg tggcgaaggt gaccccgatc
     4681 gtcgacccgc gcgtggtgca agccgaccac ggctggtggt tccccgaagc cgacccggag
     4741 gaccagggcg aaggctgctt cgcaacctat gcccgcaaca tcaacgtgtg cctgccgtcg
     4801 gaatgcggcg acaccgggtt cggcaaccag agcaagtgct atctgtgcaa ggtctacaag
     4861 tgcacgccgg aagaaatccc cctgcctgaa ccggcacagc ccaaggaccg cttcgccgag
     4921 ggcagtttcc gcaactccac cctcgaagca gaaatggagg actagccatg gctgagaagg
     4981 catacggact gctcatcaac tacgagttct gcaccggctg cggcacctgc gagatggcct
     5041 gttccgtcga gcacgacatc cccatcgggc gttacggcat ccagatggcc accatcggcc
     5101 cctggcagat cgacaagaag aactggcagc tcgacacggt gcccattccc accgacgagt
     5161 gcgacctgtg cgccaagcgc gttgagaagg gcaagaagcc aacgtgtgtg aagcactgcc
     5221 agtcgctgtg catggaatac gggcccgtcg acgagctgac ggcgaagctg gctggcgcca
     5281 cgacgaagca cgtgctcttc gtacccaagg cgggcacggc agcaaagcag taagcgttta
     5341 accctggcgc tgagcagatt tcactgaaat attccgcgaa aacaaccgct cagcgccggg
     5401 tcaaagtgca aagaggaaag ggtgggggcc tgtgtgcggt gggtatacgc gcgtatgcgt
     5461 acgttgcggt ctatgcggca aggtcgaccc tcgcccgctc aacactgcag gcgcctgtcc
     5521 gatgtgcggg caggtcgccg agccgggagc gcagcattgc agcgtctgcg gcgccagaat
     5581 cccccgggcg cccggcgagg aggcacaccc gagcaatgct cgtcccgaca ttacgggaat
     5641 caacgggaaa caggccgaag aggccggaag ggagaaacga tgatcgcgat ggaagacgtc
     5701 aagccgattg aagtggaagc atcgatccca gccgagcagg cggccgcccc aagcaactcc
     5761 ctcaaacgca agaggccgga aggcgaaggc gcggtcgaga tctactgcac caagtgcggc
     5821 gagtcggcga ccgacgggcg cttcagcccc tatggcccga gcatggagtg gtggtacacc
     5881 atcgccggaa ccctcgacga gatccagtgg gtctatcaga cggacaagaa ctactgggcg
     5941 aaggtggtcg agccgaccgt tcgatgcgga tgcggtagcg ggcgcgagtg caaccccatg
     6001 ctgtggtccg tgcggcagat tcccgcaagc tagtcgactc acactggctc accttcgggt
     6061 gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc
     6121 ttttttatta tttaattcct gttataaaaa aaggatcaat tttgaactct ctcccaaagt
     6181 tgatccctta acgatccctg caggagaagg ccatcctgac ggatggcctt tttgaagctt
     6241 tctagaatcg ggggcccccg gggggattct agaacaatgg aagtcctcgt ctcgccctcg
     6301 aattagcccg cttaatgagc gggctttttt tgaattaatt ctcgcgagct ggcacgacag
     6361 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt
     6421 gcaagctggc cgacgcgctg ggctacgtct tgctggcgtt cgggagcaga agagcataca
     6481 tctggaagca aagccaggaa agcggcctat ggagctgtgc ggcagcgctc agtaggcaat
     6541 ttttcaaaat attgttaagc cttttctgag catggtattt ttcatggtat taccaattag
     6601 caggaaaata agccattgaa tataaaagat aaaaatgtct tgtttacaat agagtggggg
     6661 gggtcagcct gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac
     6721 cgtccagccc agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg
     6781 catggtcgaa ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc
     6841 cttgccggtt ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt
     6901 ttcgctgtcc agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat
     6961 gacggcctgc gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct
     7021 ggcgtactcc gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga
     7081 taccttccaa aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac
     7141 ctctgccccg atttcctttg ccagcgcccg atagctacct ttgaccacca tggcatcagc
     7201 ggtgacggcc tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt
     7261 gggttccggg ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat
     7321 gcgcagatca tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc
     7381 gtagtcatac gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa
     7441 caggccgggg gccagacagt gcgccgggtc gtgtcggacg tggctgaggc tgtgcttgtt
     7501 cttaggcttc accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct
     7561 tgagcacccc gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg
     7621 ccttgctcac accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct
     7681 cggcctcggc gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta
     7741 ccgagctgcc ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc
     7801 tggcatggtg caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac
     7861 cgatgacctg ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg
     7921 tgtccagcac catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg
     7981 gggccatgat gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg
     8041 cttgccgttc ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg
     8101 tgggcgggtc ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca
     8161 gcagatccgg cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg
     8221 caccaccggg cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt
     8281 ccagcggtgg cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca
     8341 ttgattgcct cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc
     8401 ctgcgccgct ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag
     8461 ccagaacttg cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg
     8521 tacagcgtca gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc
     8581 agtcaccgag aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc
     8641 agccgtcaag gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt
     8701 ttgacgtata accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga
     8761 ccgaccctga agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat
     8821 agtataacag gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt
     8881 ccatgcctcg ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc
     8941 tgggcagacc catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct
     9001 tgcgctctgc cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc
     9061 ggccccggct ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga
     9121 agcgcttgac cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc
     9181 ggctaagctg ccgctcggac agttcgaggc tggccagcct gcgggccttc tcctgctgcc
     9241 gctgggcctg ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg
     9301 tcttgccctt ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct
     9361 tgtccttgcg gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg
     9421 ggtcggcgtc gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg
     9481 cggcgtcggc caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg
     9541 caggctcccg gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca
     9601 gcaccagacc atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat
     9661 tcatccgctt gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca
     9721 tcatctggcc ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct
     9781 tgagctgtcg gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag
     9841 ccagatcgag ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat
     9901 cagcagcacc accgtaggca tcatggaagc cagcatcacg gttagccata gcttccagtg
     9961 ccacccccgc gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg cggctacctc
    10021 ccgcaactct ttggccagct ccacccatgc cgcccctgtc tggcgctggg ctttcagcca
    10081 ctccgccgcc tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc gggcttcgtc
    10141 ggccagtgtc gccatgctct gggccagcgg ttcgatctgc tccgctaact cgttgatgcc
    10201 tctggatttc ttcactctgt cgattgcgtt catggtctat tgcctcccgg tattcctgta
    10261 agtcgatgat ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc
    10321 ggatgccccg gccttccatc tccaccacgt tcggccccag gtgaacaccg ggcaggcgct
    10381 cgatgccctg cgcctcaagt gttctgtggt caatgcgggc gtcgtggcct gcccgctcta
    10441 atgcccggtt ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat gccttgggct
    10501 tgagcgcttc ggtcttctgt gccccgccct tctccggggt cttgccgttg taccgcttga
    10561 accactgagc ggcgggccgc tcgatgccgt cattgatccg ctcggagatc atcaggtggc
    10621 agtgcgggtt ctcgccgcca ccggcatgga tggccagcgt atacggcagg cgctcggcac
    10681 cggtcaggtg ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg gtcagctcga
    10741 ccggcagggc aaattcgacc tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg
    10801 cagcatccca gtagtcggcg ggccgctcga cgaactccgg catgtgcccg gattcggcgt
    10861 gcaagacttc atccatgtcg cgggcatact tgccttcgcg ctggatgaag tcggccttgg
    10921 ccctggccga ttggccgccc gacctgctgc cggttttcgc cgtaaggtga taaatcgcca
    10981 tgctgcctcg ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc
    11041 gcccgaaggg tggccgttag gccagtttct cgaagagaaa ccggtaagtg cgccctcccc
    11101 tacaaagtag ggtcgggatt gccgccgctg tgcctccatg atagcctacg agacagcaca
    11161 ttaacaatgg ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc ggctggccaa
    11221 gctcgaagaa caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg caagggaaca
    11281 gcagcaagag cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca tgattttggc
    11341 caaggtgaac agcagcgagt ggccggagga tcggctcatg gcggcaatgg atgcgtacct
    11401 tgaacgcgac cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg atgagccggg
    11461 ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac gcgcccccac ccttcgggta
    11521 gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt
    11581 ggggtttagc gggctttgcc cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag
    11641 cctagcgcag cgaatagacc agctatccgg cctctggccg ggcatattgg gcaagggcag
    11701 cagcgcccca caagggcgct gataaccgcg cctagtggat tattcttaga taatcatgga
    11761 tggatttttc caacaccccg ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt
    11821 gacagttatt gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg
    11881 gttcgtgaca gttagtacgg gagtgacggg cactggctgg caatgtctag caacggcagg
    11941 catttcggct gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta
    12001 ttgcaaggac gcggaacatg cctcatgtgg cggccctcgc gagaattaat tcagataaaa
    12061 aaaatcctta gctttcgcta aggatgattt ctagcgatga ccctgctgat tggttcgctg
    12121 accgtagact ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
    12181 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
    12241 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
    12301 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
    12361 gtgcgacatc gcgcgcgcgc gcgcggatgc tagcggcgcg ccaaaaaaaa gcccgctcat
    12421 ta
//
