LOCUS       plasmid                 7847 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(4445..5539)
                     /note="pLannotate"
                     /label="LEU2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             join(7562..7847,1..374)
                     /note="pLannotate"
                     /label="CmR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(6275..6703)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(5540..5947)
                     /note="pLannotate"
                     /label="LEU2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      1238..1485
                     /note="pLannotate"
                     /label="CYC1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    7329..7453
                     /note="pLannotate"
                     /label="attR1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1062..1186)
                     /note="pLannotate"
                     /label="attR2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(3583..3687)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2722..3582)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             716..1018
                     /note="pLannotate"
                     /label="ccdB"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1963..2551)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     5'UTR           7242..7299
                     /note="pLannotate"
                     /label="AtADH 5'-UTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="5'UTR"
     intron          join(7532..7847,1..462)
                     /note="pLannotate"
                     /label="PDK intron (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="48.7"
                     /fragment="True"
                     /other="intron"
     promoter        7478..7508
                     /note="pLannotate"
                     /label="lac UV5 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1609..1639)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1654..1675)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        6867..6885
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1522..1540)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1585..1601)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(6707..6864)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             553..711
                     /note="pLannotate"
                     /label="ccdA (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="73.6"
                     /fragment="True"
                     /other="CDS"
     promoter        6964..7009
                     /note="pLannotate"
                     /label="minimal CaMV 35S promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="promoter"
     ncRNA           2406..2510
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
     misc_feature    1190..1237
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="44.4"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    6195..6247
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    3919..3969
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           complement(5890..6003)
                     /note="pLannotate"
                     /label="tRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="160.6"
                     /fragment="False"
                     /other="ncRNA"
     CDS             complement(6093..6164)
                     /note="pLannotate"
                     /label="YO192_YEAST (fragment)"
                     /database="swissprot"
                     /identity="79.2"
                     /match_length="30.8"
                     /fragment="True"
                     /other="CDS"
     promoter        7532..7561
                     /note="pLannotate"
                     /label="cat promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="promoter"
     promoter        complement(1583..1601)
                     /note="pLannotate"
                     /label="T5 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="42.2"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(1688..1780)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3782..3817
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3968..4084
                     /note="pLannotate"
                     /label="NFS1 (fragment)"
                     /database="swissprot"
                     /identity="97.4"
                     /match_length="7.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    7305..7325
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.4"
                     /fragment="True"
                     /other="misc_feature"
     intron          6867..6886
                     /note="pLannotate"
                     /label="CMV intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.2"
                     /fragment="True"
                     /other="intron"
     CDS             complement(1594..1689)
                     /note="pLannotate"
                     /label="penA (fragment)"
                     /database="swissprot"
                     /identity="68.8"
                     /match_length="10.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
       61 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      121 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
      181 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
      241 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
      301 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
      361 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
      421 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
      481 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
      541 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
      601 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
      661 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
      721 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
      781 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
      841 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
      901 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
      961 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     1021 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     1081 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     1141 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatg ggctgcagga
     1201 attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc
     1261 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     1321 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     1381 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     1441 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg tcgagggggg
     1501 gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     1561 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1621 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1681 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1741 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1801 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1861 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1921 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1981 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2041 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2101 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2161 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2221 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2281 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2341 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2401 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2461 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2521 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2581 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2641 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2701 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2761 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2821 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2881 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2941 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3001 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3061 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3121 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3181 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3241 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3301 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3361 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3421 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3481 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3541 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3601 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3661 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3721 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3781 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3841 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3901 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3961 gcaccatatc gactacgtcg ttaaggccgt ttctgacaga gtaaaattct tgagggaact
     4021 ttcaccatta tgggaaatgg ttcaagaagg tattgactta aactccatca aatggtcagg
     4081 tcattgagtg ttttttattt gttgtatttt ttttttttag agaaaatcct ccaatatata
     4141 aattaggaat catagtttca tgattttctg ttacacctaa ctttttgtgt ggtgccctcc
     4201 tccttgtcaa tattaatgtt aaagtgcaat tctttttcct tatcacgttg agccattagt
     4261 atcaatttgc ttacctgtat tcctttacat cctccttttt ctccttcttg ataaatgtat
     4321 gtagattgcg tatatagttt cgtctaccct atgaacatat tccattttgt aatttcgtgt
     4381 cgtttctatt atgaatttca tttataaagt ttatgtacaa atatcataaa aaaagagaat
     4441 ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac
     4501 tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat
     4561 cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag
     4621 acaagatagt ggcgataggg ttgaccttat tctttggcaa atctggagca gaaccgtggc
     4681 atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg
     4741 gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca
     4801 tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg
     4861 cagaatcaat caattgatgt tgaaccttca atgtagggaa ttcgttcttg atggtttcct
     4921 ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa
     4981 taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca
     5041 cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc
     5101 tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag
     5161 caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc
     5221 ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa
     5281 cactaccggt accccattta ggaccaccca cagcacctaa caaaacggca tcagccttct
     5341 tggaggcttc cagcgcctca tctggaagtg gaacacctgt agcatcgata gcagcaccac
     5401 caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa
     5461 gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga
     5521 tcttcttagg ggcagacatt agaatggtat atccttgaaa tatatatata tatattgctg
     5581 aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa
     5641 acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc atttagtcat
     5701 gaacgcttct ctattctata tgaaaagccg gttccggcgc tctcaccttt cctttttctc
     5761 ccaatttttc agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt
     5821 tccagtcatc gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg
     5881 aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc
     5941 cacagttaac tgcggtcaag atatttcttg aatcaggcgc cttagaccgc tcggccaaac
     6001 aaccaattac ttgttgagaa atagagtata attatcctat aaatataacg tttttgaaca
     6061 cacatgaaca aggaagtaca ggacaattga ttttgaagag aatgtggatt ttgatgtaat
     6121 tgttgggatt ccatttttaa taaggcaata atattaggta tgtagatata ctagaagttc
     6181 tcctcgaccg gtcgatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6241 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6301 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6361 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6421 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6481 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6541 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     6601 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     6661 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     6721 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6781 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6841 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttggagctct
     6901 tcgagtttac cgcggccgcc cccttcacct gaatcagggt gaagaaaaac agcggcggtt
     6961 ctcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggt atttttacaa
     7021 caattaccaa caacaacaaa caacaacgcc aagtggaaag agcgttcgag agaacaaggc
     7081 aaaaccaaat acgcccctag tattctacag atgtcgactg gataattaca aaagatttca
     7141 ataaacagta ctaattaatt tctagtggtg agtttttgta aatatctact tcttccaatt
     7201 accagctgct atataaatcc ccttctctgt ttctcttttc ttacatcaca atcacacaaa
     7261 actaacaaaa gatcaaaagc aagttcttca ctgttgatac ggattctaga actagtggat
     7321 cccccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     7381 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     7441 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     7501 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     7561 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     7621 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     7681 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     7741 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     7801 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttcc
//
