LOCUS       plasmid                 6369 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             167..829
                     /note="pLannotate"
                     /label="HIS3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2436..3095
                     /note="pLannotate"
                     /label="CmR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1116..1544)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    2203..2327
                     /note="pLannotate"
                     /label="attR1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(3783..3907)
                     /note="pLannotate"
                     /label="attR2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(6046..6150)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(5185..6045)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3437..3739
                     /note="pLannotate"
                     /label="ccdB"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4426..5014)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     5'UTR           2116..2173
                     /note="pLannotate"
                     /label="AtADH 5'-UTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="5'UTR"
     intron          2406..3183
                     /note="pLannotate"
                     /label="PDK intron (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="48.7"
                     /fragment="True"
                     /other="intron"
     promoter        2352..2382
                     /note="pLannotate"
                     /label="lac UV5 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(4072..4102)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4117..4138)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1708..1726
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3985..4003)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4048..4064)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1..166
                     /note="pLannotate"
                     /label="HIS3 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="88.3"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(1548..1705)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3274..3432
                     /note="pLannotate"
                     /label="ccdA (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="73.6"
                     /fragment="True"
                     /other="CDS"
     promoter        1838..1883
                     /note="pLannotate"
                     /label="minimal CaMV 35S promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3911..3973
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.3"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           4869..4973
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
     misc_feature    1037..1088
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     promoter        2406..2435
                     /note="pLannotate"
                     /label="cat promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="promoter"
     promoter        complement(4046..4064)
                     /note="pLannotate"
                     /label="T5 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="42.2"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(4151..4243)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             6245..6280
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2179..2199
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.4"
                     /fragment="True"
                     /other="misc_feature"
     intron          1708..1727
                     /note="pLannotate"
                     /label="CMV intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.2"
                     /fragment="True"
                     /other="intron"
     CDS             complement(4057..4152)
                     /note="pLannotate"
                     /label="penA (fragment)"
                     /database="swissprot"
                     /identity="68.8"
                     /match_length="10.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3913..3972
                     /note="pLannotate"
                     /label="H1L1_OSTED (fragment)"
                     /database="swissprot"
                     /identity="90.0"
                     /match_length="9.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgcctcggt aatgattttc attttttttt ttccacctag cggatgactc tttttttttc
       61 ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg tgatttcttc
      121 gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga cagagcagaa
      181 agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct ctttaaaggg
      241 tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag cagtagcaga
      301 acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc tggaccatat
      361 gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca ttggtgactt
      421 acacatagac gaccatcaca ccactgaaga ctgcgggatt gctctcggtc aagcttttaa
      481 agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg cgcctttgga
      541 tgaggcactt tccagagcgg tggtagatct ttcgaacagg ccgtacgcag ttgtcgaact
      601 tggtttgcaa agggagaaag taggagatct ctcttgcgag atgatcccgc attttcttga
      661 aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag gcaagaatga
      721 tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag aagccacctc
      781 gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt gacaccgatt
      841 atttaaagct gcagcatacg atatatatac atgtgtatat atgtatacct atgaatgtca
      901 gtaagtatgt atacgaacag tatgatactg aagatgacaa ggtaatgcat cattctatac
      961 gtgtcattct gaacgaggcg cgctttcctt ttttcttttt gctttttctt tttttttctc
     1021 ttgaactcga cggatctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1081 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     1141 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     1201 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1261 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1321 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1381 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1441 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1501 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     1561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1621 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1681 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
     1741 ttcgagttta ccgcggccgc ccccttcacc tgaatcaggg tgaagaaaaa cagcggcggt
     1801 tcttgaatca gggtgaagaa aaacagcggc ggttctcgca agacccttcc tctatataag
     1861 gaagttcatt tcatttggag aggtattttt acaacaatta ccaacaacaa caaacaacaa
     1921 cgccaagtgg aaagagcgtt cgagagaaca aggcaaaacc aaatacgccc ctagtattct
     1981 acagatgtcg actggataat tacaaaagat ttcaataaac agtactaatt aatttctagt
     2041 ggtgagtttt tgtaaatatc tacttcttcc aattaccagc tgctatataa atccccttct
     2101 ctgtttctct tttcttacat cacaatcaca caaaactaac aaaagatcaa aagcaagttc
     2161 ttcactgttg atacggattc tagaactagt ggatccccca tcacaagttt gtacaaaaaa
     2221 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     2281 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     2341 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     2401 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     2461 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     2521 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     2581 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     2641 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     2701 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     2761 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     2821 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     2881 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     2941 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3001 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3061 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     3121 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     3181 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     3241 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     3301 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     3361 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     3421 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     3481 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     3541 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     3601 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     3661 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     3721 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     3781 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     3841 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     3901 aagtggtgat gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg
     3961 ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     4021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6241 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6301 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6361 gggtgttgg
//
