LOCUS       PBCAG-eGFP-POLQ-10A2-F 14514 bp ds-DNA     circular     12-MAR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7..241
                     /label="Piggybac right ITR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_recomb     complement(370..403)
                     /label="LoxP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    499..802
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    805..1080
                     /label="Chicken beta actin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1081..2089
                     /label="Chimeric intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2182..2187
                     /label="Kozak consensus"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2191..2904
                     /label="GFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2917..10686
                     /label="Translation 2917-10686"
                     /translation="SNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYEIDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQRCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNIARLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETALNMADALLFDAFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDEAIIFSEMDAVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHAFIWSGAAFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILEAPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGLSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV"
     misc_feature    2920..10686
                     /label="POLQ"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     mut             6781..6783
                     /label="S1289A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    7360..7362
                     /label="S1482A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7372..7374
                     /label="S1486A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    7378..7380
                     /label="S1488A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7393..7395
                     /label="S1493A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7579..7581
                     /label="S1555A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7603..7605
                     /label="S1563A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7798..7800
                     /label="S1628A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7819..7821
                     /label="S1635"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    8179..8181
                     /label="T1755A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    10687..10710
                     /label="Flag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    10713..10776
                     /label="P2A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    10777..11175
                     /label="Blast"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     polyA_signal    11249..11304
                     /label="beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    11698..12010
                     /label="Piggybac left ITR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(12025..12045)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(12051..12073)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(12077..12107)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(12746..13428)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(13526..14185)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gtcgacttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
       61 aaattgacgc atgtgtttta tcgatctgta tatcgaggtt tatttattaa tttgaataga
      121 tattaagttt tattatattt acacttacat actaataata aattcaacaa acaatttatt
      181 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      241 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      301 tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca aattatactt
      361 attaatcgca taacttcgta taatgtatgc tatacgaagt tatgcgatta agggatctgt
      421 agggcgcagt agtccaacta gttattaata gtaatcaatt acggggtcat tagttcatag
      481 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      541 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      601 gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca
      661 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      721 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      781 attagtcatc gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca
      841 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      901 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      961 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     1021 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     1081 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
     1141 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     1201 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
     1261 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt
     1321 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc
     1381 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg
     1441 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1501 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc
     1561 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg
     1621 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1681 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg
     1741 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1801 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct
     1861 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1921 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga
     1981 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     2041 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     2101 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtaccg
     2161 cgggcccggg atccaCCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
     2221 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
     2281 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
     2341 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
     2401 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
     2461 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
     2521 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
     2581 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
     2641 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
     2701 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
     2761 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
     2821 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
     2881 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCa atcttctgcg tcggagtggg
     2941 aaacggcggc gttcagaatc aggctcagat tcgttctcgg gaagcggcgg tgacagcagt
     3001 gccagccccc agttcctctc cgggtccgtg ctgagcccgc cgcccggcct tggtcgctgc
     3061 ctgaaggccg cagctgcagg agaatgcaag cctacagttc ctgactacga aaTagacaag
     3121 ctactattgg caaactgggg acttcctaaa gcagttctgg aaaaatacca cagttttggt
     3181 gtaaaaaaga tgtttgaatg gcaggcagag tgccttttgc ttggacaagt cctggaagga
     3241 aagaatttag tttattcagc tcctacaagt gctgggaaga ctcttgtggc agaattactt
     3301 attttgaagc gggttttgga aatgcggaag aaagctttgt ttattcttcc ctttgtttct
     3361 gtggctaaag agaagaaata ctacctccag agtctgtttc aggaagtagg aataaaagta
     3421 gacggttata tgggcagcac ctctccatca aggcatttct cttcattgga tattgcagtc
     3481 tgcacaattg agagagccaa tggtctgatc aatcgcctca tagaggaaaa taagatggat
     3541 ctgttaggaa tggtggttgt ggatgaatta catatgctgg gagactctca ccgagggtat
     3601 ctgctggaac ttttgctgac caagatttgc tatattactc ggaaatcagc atcttgtcag
     3661 gcagatctag ccagttctct gtctaatgct gtgcaaatcg ttggcatgag tgctaccctt
     3721 cctaatttgg agcttgtggc ttcctggttg aatgctgaac tctaccatac cgactttcgc
     3781 cctgtaccgc ttttggagtc agtaaaagtt ggaaattcca tatatgactc ttcaatgaaa
     3841 cttgtgaggg aatttgagcc catgctTcaa gtgaagggag atgaggacca tgttgttagC
     3901 ttatgttatg agacgatttg tgataaccat tcagtattac ttttttgtcc atcaaagaaa
     3961 tggtgtgaga agctggcaga tatcattgcA cgagagtttt ataatctaca tcatcaagct
     4021 gagggattgg tgaaaccctc tgaatgccca ccagtaattc tggaacaaaa agaactcctg
     4081 gaagtgatgg atcagttaag acgGttgcct tcaggactgg actctgtatt acagaaaact
     4141 gtaccatggg gagtagcatt tcatcatgca ggtcttactt ttgaggagag ggatatcatt
     4201 gaaggagcct ttcgtcaagg tctcattcgg gtcttggcgg caacttctac tctttcttct
     4261 ggggtgaatt tacctgcacg tcgtgtgatt attcgaaccc ctatttttgg tggtcgacct
     4321 ctagatattc ttacttataa gcagatggtt ggTcgtgctg gcaggaaagg agtggacaca
     4381 gtaggcgaga gtatcttaat ttgtaagaac tctgagaaat caaaaggcat agctctcctt
     4441 cagggttctc taaagcctgt tcgcagctgt ctgcaaagac gagaaggaga agaagtaact
     4501 ggcagcatga tacgagctat tctggagata atagttggtg gagtggcaag tacatcacaa
     4561 gatatgcata cttatgctgc ctgcacattt ttggctgcaa gtatgaaaga agggaagcaa
     4621 ggaattcaga gaaatcaaga gtctgttcag cttggagcga ttgaggcctg tgtgatgtgg
     4681 ctactagaaa atgaattcat ccagagtaca gaagccagtg atggaacaga aggaaaggtg
     4741 tatcatccaa cacatcttgg ttcggccact ctttcttctt cactttctcc agctgatact
     4801 ttagatattt ttgctgacct gcaaagagca atgaagggct ttgttttaga gaatgatctt
     4861 catattctct atctggttac acctatgttt gaggattgga ctactattga ttggtatcga
     4921 tttttctgtt tatgggagaa gttgccaact tcaatgaaaa gggtggcaga gctagtggga
     4981 gttgaagagg ggttcttggc ccgttgtgtg aaaggaaaag tagtagccag aactgagaga
     5041 cagcatcgac aaatggccat ccataaaagg tttttcacca gtcttgtgct attagattta
     5101 atcagtgaag ttcccttaag ggaaataaat cagaaatatg gatgcaatcg tgggcagatt
     5161 caatctttgc aacagtcagc tgctgtttat gcagggatga ttacagtatt ttccaaccgt
     5221 ctgggctggc acaacatgga actactactt tcccaatttc agaagcgtct tacgtttggc
     5281 atccagaggg agctgtgtga cctggttcgg gtatccttac taaatgctca gagagccagg
     5341 gttctctatg cttctggctt tcatactgtg gcagaccttg ctagagcaaa tattgtggag
     5401 gtggaggtga ttctgaaaaa tgctgtgcct ttcaaaagtg cccggaaggc agtggatgag
     5461 gaagaggaag cagttgaaga acgtcgcaat atgcgaacta tctgggtgac tggcagaaaa
     5521 ggtttaactg aaagggaagc agcagccctt atagtggaag aagccagaat gattctgcag
     5581 caggacttag ttgaaatggg agtgcaatgg aatccatgtg ccctgttaca ttctagtaca
     5641 tgctcattga ctcatagtga gtccgaagta aaggaacaca catttatatc ccaaactaag
     5701 agttcttata aaaaattaac atcaaagaac aaaagtaaca caatatttag tgattcttat
     5761 attaagcatt caccaaatat agtgcaagac ttaaataaaa gtagagagca tacaagttcc
     5821 tttaattgta atttccagaa tgggaatcaa gaacatcaga gatgttccat tttcagagca
     5881 agaaaacggg cctctttaga tataaataaa gagaagccag gagcctctca gaatgagggg
     5941 aaaacaagtg ataagaaagt tgttcagact ttttcacaga aaacaaaaaa ggcacctttg
     6001 aatttcaatt cagaaaagat gagcagaagt tttcgatctt ggaaacgtag aaagcatcta
     6061 aagcgatcta gggacagcag ccccctgaaa gactctggag cgtgtagaat ccatttacaa
     6121 ggacagactc tgtctaatcc tagtctttgt gaagacccgt ttaccttaga tgagaagaaa
     6181 acggaattta gaaattcagg gccatttgct aaaaatgtat ctttgagtgg taaggaaaaa
     6241 gataataaaa catcattccc attacaaata aagcaaaatt gttcatggaa cataacacta
     6301 actaatgata attttgtgga gcatattgtc acaggatctc agagtaaaaa tgtgacttgt
     6361 caggccacta gtgtggttag tgaaaagggc agaggagtag ctgttgaggc agaaaaaata
     6421 aatgaagtgc tgatacaaaa tggttcaaaa aaccagaatg tttatatgaa acaccatgac
     6481 atccatccaa ttaaccagta cctgcgaaag caatctcatg aacagacaag cactattacc
     6541 aaacagAAGa atATTATCga gagacaaatg ccctgtgaag cagtcagtag ttacataaat
     6601 agagactcaa atgttactat caattgtgaa aggataaagc ttaatacaga ggaaaataaa
     6661 ccaagtcatt ttcaggcatt aggagatgat ataagcagaa ctgtgatacc cagtgaagta
     6721 cttccatcag ctggagcatt tagcaaatca gaaggccagc atgagaattt tctaaatatt
     6781 GCCagactac aagaaaaaac aggtacttat acaacaaaca aaactaaaaa taatcatgtt
     6841 tctgacttag gtttagtcct ctgtgatttt gaagatagtt tctatctgga tactcagtca
     6901 gagaaaataa tacaacagat ggcaactgaa aatgccaaac taggagcaaa ggacaccaac
     6961 ctggcagcag ggataatgca gaagagctta gtccaacaga actcaatgaa ctcttttcag
     7021 aaggagtgtc acattccttt tcctgctgaa cagcaccctc taggagcgac taagatagat
     7081 catttggacc ttaagactgt aggtactatg aaacaaagca gtgattcaca tggggttgat
     7141 atcctgactc cagaaagccc gattttccat tctccaatac tattggagga aaatggtctt
     7201 tttttaaaaa agaatgaagt ttctgttact gattcacaat taaatagttt tcttcaaggt
     7261 tatcaaacac aagaaactgt gaaaccagtt atacttctga ttcctcaaaa gagaactccc
     7321 actggtgtag aaggagaatg tcttccagtt cctgaaacaG CTttgaatat gGCAgatGCG
     7381 ttactatttg atGCTttcag tgatgactat ctagtaaaag aacaattacc tgatatgcaa
     7441 atgaaagaac cccttccttc agaagtaaca tcaaaccatt ttagtgattc tctgtgtcta
     7501 caagaagacc taattaaaaa atcaaatgta aatgagaatc aagataccca ccagcagttg
     7561 acttgttcca atgatgaaGC Tattatattt tcagaaatgg atGCCgttca gatggttgaa
     7621 gctttggaca atgtggatat atttcctgtc caagagaaga atcatactgt agtatctcct
     7681 agagcattag aactaagtga tccagtactt gatgagcacc accaaggtga tcaagatgga
     7741 ggagatcaag atgaaagggc tgaaaaatca aaattaactg ggaccaggca aaatcatGCA
     7801 ttcatttggt caggggcaGC Atttgatcta agtccaggac tgcaaaggat tttagataaa
     7861 gtatccagtc ctctagaaaa tgaaaagcta aaatcaatga ctataaactt ttccagtttg
     7921 aatagaaaaa atacagagtt aaatgaagaa caagaagtta tttcaaactt ggagacaaaa
     7981 caagtgcagg gaatttcatt ttcttctaat aatgaagtaa aaagcaagat tgagatgcta
     8041 gaaaacaatg ccaatcatga tgaaacctca tccctcttac ctcgtaaaga aagtaatata
     8101 gttgatgata atggtctcat tcctcctaca cccattccaa catctgcttc taagctgaca
     8161 tttccaggga ttcttgaaGC Gcctgtaaac ccttggaaaa ctaataatgt tttacaacct
     8221 ggtgaaagtt atttatttgg ctcaccttca gatattaaaa accacgattt aagtccaggg
     8281 agtagaaatg ggttcaaaga caacagccct attagtgaca caagcttttc acttcagtta
     8341 tcacaggatg gattacagtt aactccagcc tcaagcagtt cagaaagttt gtccataatt
     8401 gatgtagcaa gtgaccaaaa tcttttccaa acattcatta aggagtggcg gtgcaaaaag
     8461 cgattttcca tctcactggc ttgtgaaaag attagaagtt tgacatcttc taaaactgct
     8521 actattggca gtaggtttaa gcaagctagc tcacctcagg aaattcctat tagagatgat
     8581 ggatttccca ttaaaggttg tgatgacacc ttggtggttg gactggcagt atgctggggt
     8641 ggaagggatg cctattattt ttcactgcag aaggaacaaa agcattctga aattagtgcc
     8701 agtttggttc caccttcttt agatccaagc ctgactttga aagacaggat gtggtacctt
     8761 caatcttgct tgcgaaagga atctgataaa gaatgttctg ttgtcatcta tgacttcatc
     8821 cagagctata aaattcttct tctttcttgt ggcatctcct tggagcaaag ttatgaagat
     8881 cctaaggtgg catgctggtt actagatcca gattctcagg agccgactct tcatagcata
     8941 gttaccagtt ttcttcctca tgagcttcca ctcctagaag ggatggagac cagccaaggg
     9001 attcaaagcc tggggctaaa tgctggcagt gagcattctg ggcgatacag agcatctgtg
     9061 gagtccattc tcatcttcaa ctctatgaat cagctcaact ctttgttgca gaaggaaaac
     9121 cttcaagatg ttttccgtaa ggtggaaatg ccctctcagt actgcttggc cttgctagaa
     9181 ctaaatggaa ttggctttag tactgcagaa tgtgaaagtc agaaacatat aatgcaagcc
     9241 aagctggatg caattgagac ccaggcctat caactagctg gccacagttt ttctttcacc
     9301 agttcagatg acatcgctga ggttttattt ttggaattga agttgccccc aaatagagag
     9361 atgaaaaacc aaggcagcaa gaaaactctg ggttctacca gaagagggat tgacaatgga
     9421 cgcaagctaa ggctgggaag acagttcagc actagtaagg acgttttaaa taaattaaag
     9481 gcattacatc ctttaccagg cttgatatta gaatggagaa gaatcactaa tgctattacc
     9541 aaagtggtct ttccccttca gcgggaaaag tgtcttaatc cttttcttgg aatggaaaga
     9601 atctatcctg tatcacagtc gcacactgct acaggacgaa taacctttac agaaccaaat
     9661 attcagaatg tgccaagaga ttttgaaatc aaaatgccaa cactagtagg agaaagccca
     9721 ccttctcaag ctgtaggcaa aggcctactt cccatgggca gaggaaaata taagaagggt
     9781 ttAagcgtga atcctagatg ccaggcacag atggaggaga gagctgcaga cagaggaatg
     9841 ccattttcaa ttagcatgcg acatgccttt gtgcctttcc caggtggttc aatactggct
     9901 gctgactact ctcagcttga actgaggatc ttggctcatt tatcccatga tcgtcgtctc
     9961 attcaagtgt taaacactgg agctgatgtt ttcaggagca ttgcagcaga gtggaagatg
    10021 attgagccag agtctgttgg ggatgatctg aggcagcagg caaaacagat ttgctatggg
    10081 atcatttatg gaatgggagc taaatctttg ggagagcaga tgggcattaa agaaaatgat
    10141 gctgcatgct atattgactc cttcaaatcc agatacacag ggattaatca attcatgaca
    10201 gagacagtga agaattgtaa aagagacgga tttgttcaga ccattttggg aaggcgtaga
    10261 tatttgccag gaatcaaaga caacaaccct tatcgtaaag ctcacgctga gcgtcaagct
    10321 atcaacacaa tagtccaagg atcagcagct gatattgtca aaatagccac agttaacatt
    10381 cagaagcaat tagagacctt ccactcaacc ttcaaatccc atggtcatcg agagggtatg
    10441 ctccaaagtg accGaacagg attgtcacga aagagaaaac tgcaagggat gttctgccca
    10501 atcagaggag gcttcttcat ccttcaactc catGATGAAc tcctatatga agtggcagaa
    10561 gaagatgttg ttcaggtagc tcagattgtc aagaatgaaa tggaaagtgc tgtaaaactg
    10621 tctgtgaaat tgaaagtgaa agtgaaaata ggcgccagct ggggagagct aaaggacttt
    10681 gatgtgGACT ACAAAGACGA TGACGACAAG GCAAGCGGAG CTACTAACTT CAGCCTGCTG
    10741 AAGCAGGCTG GAGACGTGGA GGAGAACCCT GGACCTATGG CCAAGCCTTT GTCTCAAGAA
    10801 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
    10861 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
    10921 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
    10981 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
    11041 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG
    11101 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
    11161 GTGTGGGAGG GCTAACTCGA Ggatcttttt ccctctgcca aaaattatgg ggacatcatg
    11221 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
    11281 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
    11341 catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga
    11401 acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
    11461 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
    11521 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
    11581 ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca
    11641 ggctccagcc tacacaatcg ctcaagacgt gtaatgcttt tattatatat tagtcacgat
    11701 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
    11761 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
    11821 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
    11881 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
    11941 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
    12001 ctagggttaa aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    12061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    12121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    12181 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
    12241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    12301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    12361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
    12421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    12481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
    12541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    12601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    12661 actcaaaggc ggtcacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    12721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    12781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    12841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    12901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
    12961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    13021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    13081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    13141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    13201 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    13261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    13321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    13381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    13441 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    13501 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    13561 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    13621 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    13681 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    13741 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    13801 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    13861 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    13921 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    13981 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    14041 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    14101 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    14161 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    14221 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    14281 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    14341 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    14401 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    14461 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//