LOCUS       plasmid                 3813 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(441..1250)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2170..3243)
                     /note="pLannotate"
                     /label="RAP2-12"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    1373..1472
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(3246..3345)
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     gene            complement(437..1226)
                     /note="pLannotate"
                     /label="kanMX (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="58.2"
                     /fragment="True"
                     /other="gene"
     terminator      complement(3401..3444)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      3576..3603
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     rep_origin      complement(1..298)
                     /note="pLannotate"
                     /label="ori (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="50.6"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(1477..2169)
                     /note="pLannotate"
                     /label="GAL4 (fragment)"
                     /database="swissprot"
                     /identity="84.8"
                     /match_length="26.2"
                     /fragment="True"
                     /other="CDS"
     ncRNA           153..257
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
     rep_origin      complement(3738..3812)
                     /note="pLannotate"
                     /label="ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.7"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
       61 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      361 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg ccagtgttac
      421 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      481 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      541 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      601 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      661 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
      721 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      781 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
      841 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
      901 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
      961 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1021 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1081 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1141 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1201 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1261 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1321 gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat
     1381 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
     1441 tataatgcca actttgtaca agaaagctgg gtgttagaaa ccgttgttgt ttggatcggt
     1501 tttcaagaat ggcaaaccat cagacatatc atcctcttca gaccaatcaa aaccatgcaa
     1561 tgcatctctt ggcatgaagt ctaatgggat agtagagtta tcatgatgag cagcagaatc
     1621 aatagctgga gtaaaagctt gtggtggaaa tggatcgaag gcattttctt cagttggata
     1681 ttcaacagag actgggaagt aatccaatgg aaaatcttct tcatctctag acacggtcaa
     1741 ctgtctttga cctttgttag aagattcttc agagctggag gtagcagaaa ttctatgttg
     1801 tctcaaggtc aatggcatat cagtttcaac agaagccaat ctatcagtaa cggcatcctt
     1861 gttaacgtta tcttgaacga acaaaccagt caacaaagcc ttgatatctt gcaaggaatc
     1921 catcttcaag atcatgtcca gatcttctct tgggaaaatc aacaagaaca gctgctccaa
     1981 tctttccaac ctagattcaa cttcggtcaa atgtgctcta gtcaatggag atctcttagt
     2041 ctttggagag tatctacatt cccagttgtt cttcaaacac ttagcacact ttggcttttc
     2101 tttggagcac ttcaattttt tcagcctgca gatatcgcaa gcttgttcaa tagaggacaa
     2161 cagcttcatg aagactcctc caatcatgga atgaatttca tcaatactcc ataggtccat
     2221 agggtttgca ccattgtcct gagtcgttac agcatcttcg ttgaggaaat ccagtgaagc
     2281 gtcccattca gtgttgttgt aaggtgtctc gaaatccata gacttgagct tcttagctgg
     2341 attggcttcc tcaaagaaca gagctgagtt gttgttgttg ataaccgcag aagagatgtc
     2401 gggagttatc ggagcttgat cgctccaacc aaactccgaa caatcgaaag aattactacc
     2461 ctggtcagag ctgaaatact gatacccatt acatccagca tcaacggagt ttgtcatccc
     2521 aaactggttg ttgttgttgt tgctcacttg gtgtttctcc tccatgaaac acatattttc
     2581 aaaggagatg ttcgagttct gaaccaaagc tggacttggg ttagggttag gtttagccac
     2641 tggtttctga agattagcct tcacagagcg tttctgagaa ttagccttca tgttttcttc
     2701 agggaaattc accttagctt tagatccacg gattctccgc gctgcagcat cgtaagctct
     2761 agcagcttcc tcagctgtct tgaacgttcc aagccagatt ctagcacctt cccttggatc
     2821 acgtatctca gcagcccatt ttccccaagg acgttgccta atccctcggt actgattctt
     2881 cctcttccta tttgcagatt tctcagcgtc cccatccaag ccagtaactt tcttaccaaa
     2941 aactgaacct tccgcagcgg cggagacggc gggttttgga gtcgaagtga aaacgaatgg
     3001 tttcacatcg gcgaaaacat caccgacgtc gaaatcatca tcgcaatcga tagacgaatc
     3061 atctttgaaa ccttggaaat cagcttcgaa ctcagcgtca aaatcgaaga aattcgaacg
     3121 attcttcgag cttttcttcg atcctttcaa attcttcttc agatccggcc aaataaactc
     3181 gctagtaaca cggcgagacc tcggcggtgg aatgaaatcg gatattatag ctcctccaca
     3241 catggagcct gcttttttgt acaaagttgg cattataaaa aagcattgct catcaatttg
     3301 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggggcc cgagatccat
     3361 gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     3421 ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     3481 aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg
     3541 acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct
     3601 ttttgcgttt ctacaaactc ttcctggcta gcggtaatac ggttatccac agaatcaggg
     3661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3781 cgctcaagtc agaggtggcg aaacccgaca ggg
//
