LOCUS       plasmid                 3005 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             join(2869..3005,1..673)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1899..2621
                     /note="pLannotate"
                     /label="PCO4"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    1797..1896
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(2647..2746)
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      816..1404
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     gene            join(2893..3005,1..677)
                     /note="pLannotate"
                     /label="kanMX (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="58.2"
                     /fragment="True"
                     /other="gene"
     terminator      1698..1741
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1539..1566)
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     ncRNA           complement(855..959)
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
ORIGIN
        1 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
       61 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
      121 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
      181 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
      241 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
      301 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
      361 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
      421 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
      481 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
      541 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
      601 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
      661 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
      721 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttttc gttccactga
      781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1441 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1501 cgtattaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     1561 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     1621 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     1681 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     1741 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatctc gggccccaaa
     1801 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1861 tttttataat gccaactttg tacaaaaaag caggctccat gccttacttt gctcagaggc
     1921 tttacaatac ttgcaaggcg tctttctctt cagacggacc aataacagaa gatgctctgg
     1981 agaaggttcg caatgtcttg gagaaaatca agccatctga tgttggaatt gagcaggatg
     2041 ctcaattggc acggtccagg tctggtcctc tcaatgaacg caatggaagt aatcagtctc
     2101 ccccagcaat aaagtatctt catttgcatg agtgtgacag tttctctata ggaatcttct
     2161 gtatgccacc ttcttctatg atacctcttc ataaccatcc gggcatgacc gtgctaagca
     2221 agctcgttta tggttcaatg catgtgaagt catatgattg gctagagcct caactgaccg
     2281 aaccagagga tccatcacaa gcaagacctg ctaaactggt gaaggatact gagatgacgg
     2341 ctcaaagccc agtaacaaca ttatatccga aaagtggtgg caacattcac tgtttcaaag
     2401 ccatcaccca ttgtgctatt cttgacatct tagctccacc ttactcttca gagcatgatc
     2461 ggcattgcac ttacttccgg aaatccagaa gagaagactt accaggtgaa ttagaagtgg
     2521 atggagaagt ggtcacagac gtgacatggc ttgaggaatt tcaaccgcct gatgactttg
     2581 tgatacgacg aattccgtac agaggtcctg tcattagaac tccgaaaaaa aaacgcaaag
     2641 tgtaacaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2701 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
     2761 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2821 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2881 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2941 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     3001 cccga
//
