LOCUS       plasmid                 4253 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             join(3757..4253,1..313)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    1437..1536
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(3535..3634)
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      456..1044
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1539..1766
                     /note="pLannotate"
                     /label="UBIQ_AVESA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1767..2837
                     /note="pLannotate"
                     /label="RAP2-12"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     gene            join(3781..4253,1..317)
                     /note="pLannotate"
                     /label="kanMX (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="58.2"
                     /fragment="True"
                     /other="gene"
     terminator      1338..1381
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1179..1206)
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             2838..3530
                     /note="pLannotate"
                     /label="GAL4 (fragment)"
                     /database="swissprot"
                     /identity="84.8"
                     /match_length="26.2"
                     /fragment="True"
                     /other="CDS"
     ncRNA           complement(495..599)
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
ORIGIN
        1 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
       61 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
      121 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
      181 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
      241 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
      301 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
      361 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttttc gttccactga
      421 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      481 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      541 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      601 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      661 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      721 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      781 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      841 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      901 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      961 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1021 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1081 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1141 cgtattaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     1201 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     1261 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     1321 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     1381 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatctc gggccccaaa
     1441 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1501 tttttataat gccaactttg tacaaaaaag caggctccat gcaaattttc gtgaaaacac
     1561 tcactggtaa gaccatcact ctcgaggttg agagctctga caccatcgac aatgttaagg
     1621 caaagatcca ggacaaggaa ggcattcctc ctgaccagca aagattgatc ttcgctggta
     1681 agcagctaga agatggccgc accttggctg attacaacat ccagaaagaa tcaacactcc
     1741 acttggttct caggttaaga ggtggttgtg gaggagctat aatatccgat ttcattccac
     1801 cgccgaggtc tcgccgtgtt actagcgagt ttatttggcc ggatctgaag aagaatttga
     1861 aaggatcgaa gaaaagctcg aagaatcgtt cgaatttctt cgattttgac gctgagttcg
     1921 aagctgattt ccaaggtttc aaagatgatt cgtctatcga ttgcgatgat gatttcgacg
     1981 tcggtgatgt tttcgccgat gtgaaaccat tcgttttcac ttcgactcca aaacccgccg
     2041 tctccgccgc tgcggaaggt tcagtttttg gtaagaaagt tactggcttg gatggggacg
     2101 ctgagaaatc tgcaaatagg aagaggaaga atcagtaccg agggattagg caacgtcctt
     2161 ggggaaaatg ggctgctgag atacgtgatc caagggaagg tgctagaatc tggcttggaa
     2221 cgttcaagac agctgaggaa gctgctagag cttacgatgc tgcagcgcgg agaatccgtg
     2281 gatctaaagc taaggtgaat ttccctgaag aaaacatgaa ggctaattct cagaaacgct
     2341 ctgtgaaggc taatcttcag aaaccagtgg ctaaacctaa ccctaaccca agtccagctt
     2401 tggttcagaa ctcgaacatc tcctttgaaa atatgtgttt catggaggag aaacaccaag
     2461 tgagcaacaa caacaacaac cagtttggga tgacaaactc cgttgatgct ggatgtaatg
     2521 ggtatcagta tttcagctct gaccagggta gtaattcttt cgattgttcg gagtttggtt
     2581 ggagcgatca agctccgata actcccgaca tctcttctgc ggttatcaac aacaacaact
     2641 cagctctgtt ctttgaggaa gccaatccag ctaagaagct caagtctatg gatttcgaga
     2701 caccttacaa caacactgaa tgggacgctt cactggattt cctcaacgaa gatgctgtaa
     2761 cgactcagga caatggtgca aaccctatgg acctatggag tattgatgaa attcattcca
     2821 tgattggagg agtcttcatg aagctgttgt cctctattga acaagcttgc gatatctgca
     2881 ggctgaaaaa attgaagtgc tccaaagaaa agccaaagtg tgctaagtgt ttgaagaaca
     2941 actgggaatg tagatactct ccaaagacta agagatctcc attgactaga gcacatttga
     3001 ccgaagttga atctaggttg gaaagattgg agcagctgtt cttgttgatt ttcccaagag
     3061 aagatctgga catgatcttg aagatggatt ccttgcaaga tatcaaggct ttgttgactg
     3121 gtttgttcgt tcaagataac gttaacaagg atgccgttac tgatagattg gcttctgttg
     3181 aaactgatat gccattgacc ttgagacaac atagaatttc tgctacctcc agctctgaag
     3241 aatcttctaa caaaggtcaa agacagttga ccgtgtctag agatgaagaa gattttccat
     3301 tggattactt cccagtctct gttgaatatc caactgaaga aaatgccttc gatccatttc
     3361 caccacaagc ttttactcca gctattgatt ctgctgctca tcatgataac tctactatcc
     3421 cattagactt catgccaaga gatgcattgc atggttttga ttggtctgaa gaggatgata
     3481 tgtctgatgg tttgccattc ttgaaaaccg atccaaacaa caacggtttc taacacccag
     3541 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     3601 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
     3661 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     3721 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     3781 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     3841 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     3901 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3961 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     4021 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     4081 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     4141 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     4201 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tga
//
