LOCUS       plasmid                 3692 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(join(3004..3692,1..121))
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(1781..1880)
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    244..343
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(2273..2861)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1551..1778)
                     /note="pLannotate"
                     /label="UBIQ_AVESA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     gene            complement(join(3000..3692,1..97))
                     /note="pLannotate"
                     /label="kanMX (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="58.2"
                     /fragment="True"
                     /other="gene"
     terminator      complement(1936..1979)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      2111..2138
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(1041..1550)
                     /note="pLannotate"
                     /label="ERF071"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="99.4"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(348..1040)
                     /note="pLannotate"
                     /label="GAL4 (fragment)"
                     /database="swissprot"
                     /identity="84.8"
                     /match_length="26.2"
                     /fragment="True"
                     /other="CDS"
     ncRNA           2716..2820
                     /note="pLannotate"
                     /label="RNAI"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.9"
                     /fragment="False"
                     /other="ncRNA"
ORIGIN
        1 agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
       61 aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca
      121 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
      181 ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc tgcagctgga
      241 tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
      301 caatgctttc ttataatgcc aactttgtac aagaaagctg ggtgttagaa accgttgttg
      361 tttggatcgg ttttcaagaa tggcaaacca tcagacatat catcctcttc agaccaatca
      421 aaaccatgca atgcatctct tggcatgaag tctaatggga tagtagagtt atcatgatga
      481 gcagcagaat caatagctgg agtaaaagct tgtggtggaa atggatcgaa ggcattttct
      541 tcagttggat attcaacaga gactgggaag taatccaatg gaaaatcttc ttcatctcta
      601 gacacggtca actgtctttg acctttgtta gaagattctt cagagctgga ggtagcagaa
      661 attctatgtt gtctcaaggt caatggcata tcagtttcaa cagaagccaa tctatcagta
      721 acggcatcct tgttaacgtt atcttgaacg aacaaaccag tcaacaaagc cttgatatct
      781 tgcaaggaat ccatcttcaa gatcatgtcc agatcttctc ttgggaaaat caacaagaac
      841 agctgctcca atctttccaa cctagattca acttcggtca aatgtgctct agtcaatgga
      901 gatctcttag tctttggaga gtatctacat tcccagttgt tcttcaaaca cttagcacac
      961 tttggctttt ctttggagca cttcaatttt ttcagcctgc agatatcgca agcttgttca
     1021 atagaggaca acagcttcat attggagtct tgatagctcc ataaatttcc aatatcggtc
     1081 gccgattggt cgtcggcaac cggaatctga tagaatctca tgtaatcctc caatgccatc
     1141 aggtcctccg ataagctctc tacttggttt tccgaaatca gctcattttg attcccccct
     1201 ggtttagtat cggcttcttc ttctacttga gtgtttggga aattcagttt ggctttccgg
     1261 ccacggattt tgatggcagc aacgtcgtag gctcgagcag cttcgtcggc ggttttgaat
     1321 gtgccaagcc agacacgtac acctttgctc gggtcacgaa tctccgctgc ccatttgccc
     1381 catggcctct gccttatccc tctgtacaga ttcttcctct ctcgtttccc atctctttct
     1441 tcactcactg agacgggttt acgcttcttc ctgctgctaa cagagccgag ttgactcggt
     1501 tctgactcag atttcgacca gatgaaatca gaaatgatag ctcccccaca accacctctt
     1561 aacctgagaa ccaagtggag tgttgattct ttctggatgt tgtaatcagc caaggtgcgg
     1621 ccatcttcta gctgcttacc agcgaagatc aatctttgct ggtcaggagg aatgccttcc
     1681 ttgtcctgga tctttgcctt aacattgtcg atggtgtcag agctctcaac ctcgagagtg
     1741 atggtcttac cagtgagtgt tttcacgaaa atttgcatgg agcctgcttt tttgtacaaa
     1801 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
     1861 caaaataaaa tcattatttg gggcccgaga tccatgctag cgttaacgcg agagtaggga
     1921 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     2041 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     2101 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct
     2161 ggctagcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagct
     2941 tgcgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     3001 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     3061 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     3121 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     3181 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     3241 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     3301 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     3361 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     3421 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     3481 atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc
     3541 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3601 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3661 aaacaactct ggcgcatcgg gcttcccata ca
//
