LOCUS       C68_pAAV_Syn_CD4        6117 bp    DNA        circular     24-NOV-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     UUID: 5ee7f31c-2d8f-4ad7-859c-0b3718a4d457
COMMENT     Type: Synthetic
COMMENT     ConfirmedExperimentally: 0
COMMENT     SequenceClass: UNA
COMMENT     TransformedInto: unspecified
COMMENT     Comments: Template 155A digested with BamHI,
            NheI&lt;br>f1:gaagcttGCCACCatgCACCTGAGAATCCACGCGAGACGG&lt;br>f2:gtc
            gtgcctgagagcgcagtcgagaagaagcttGCCACCatgCACCTGAGAATC&lt;br>r1:GTATGG
            CTCCGGCcAGCTTGCGCCGCGC&lt;br>f2:GCGCGGCGCAAGCTgGCCGGAGCCATAC&lt;br>
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="ITR"
                     /locus_tag="ITR"
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    151..628
                     /label="synapsin promoter"
                     /locus_tag="synapsin promoter"
                     /locus_tag="synapsin promoter"
                     /label="synapsin promoter"
                     /ApEinfo_label="synapsin promoter"
                     /ApEinfo_fwdcolor="#4eff51"
                     /ApEinfo_revcolor="#4eff51"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3381..3521
                     /label="ITR(1)"
                     /locus_tag="ITR(1)"
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3121..3352
                     /label="PolyA"
                     /locus_tag="PolyA"
                     /locus_tag="PolyA"
                     /label="PolyA"
                     /ApEinfo_label="PolyA"
                     /ApEinfo_fwdcolor="#ff2549"
                     /ApEinfo_revcolor="#ff2549"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3109..3114
                     /label="EcoRI"
                     /locus_tag="EcoRI"
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#46ff6e"
                     /ApEinfo_revcolor="#46ff6e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    643..648
                     /label="Kozak Sequence"
                     /locus_tag="Kozak Sequence"
                     /locus_tag="Kozak Sequence"
                     /label="Kozak Sequence"
                     /ApEinfo_label="Kozak Sequence"
                     /ApEinfo_fwdcolor="#00f900"
                     /ApEinfo_revcolor="#00f900"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    637..642
                     /label="HindIII"
                     /locus_tag="HindIII"
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#ff9200"
                     /ApEinfo_revcolor="#ff9200"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    649..651
                     /label="START Codon"
                     /locus_tag="START Codon"
                     /locus_tag="START Codon"
                     /label="START Codon"
                     /ApEinfo_label="START Codon"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3103..3108)
                     /label="double stop"
                     /locus_tag="double stop"
                     /locus_tag="double stop"
                     /label="double stop"
                     /ApEinfo_label="double stop"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    727..732
                     /label="BglII"
                     /locus_tag="BglII"
                     /locus_tag="BglII"
                     /label="BglII"
                     /ApEinfo_label="BglII"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    787..1464
                     /label="human CD4"
                     /locus_tag="human CD4"
                     /locus_tag="human CD4"
                     /label="human CD4"
                     /ApEinfo_label="human CD4"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    733..759
                     /label="HA tag 1"
                     /locus_tag="HA tag 1"
                     /locus_tag="HA tag 1"
                     /label="HA tag 1"
                     /ApEinfo_label="HA tag 1"
                     /ApEinfo_fwdcolor="#3a23ff"
                     /ApEinfo_revcolor="#3a23ff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    760..786
                     /label="HA tag 2"
                     /locus_tag="HA tag 2"
                     /locus_tag="HA tag 2"
                     /label="HA tag 2"
                     /ApEinfo_label="HA tag 2"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    652..711
                     /label="Ig k-chain signal sequence"
                     /locus_tag="Ig k-chain signal sequence"
                     /locus_tag="Ig k-chain signal sequence"
                     /label="Ig k-chain signal sequence"
                     /ApEinfo_label="Ig k-chain signal sequence"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1495..1533
                     /label="Linker"
                     /locus_tag="Linker"
                     /locus_tag="13 aa Linker"
                     /label="13 aa Linker"
                     /ApEinfo_label="13 aa Linker"
                     /ApEinfo_fwdcolor="#ff9200"
                     /ApEinfo_revcolor="#ff9200"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1534..2046
                     /label="CIBN"
                     /locus_tag="CIBN"
                     /locus_tag="CIBN"
                     /label="CIBN"
                     /ApEinfo_label="CIBN"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2047..2061
                     /label="5 aa GS linker"
                     /locus_tag="5 aa GS linker"
                     /locus_tag="5 aa GS linker"
                     /label="5 aa GS linker"
                     /ApEinfo_label="5 aa GS linker"
                     /ApEinfo_fwdcolor="#aa7941"
                     /ApEinfo_revcolor="#aa7941"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1465..1494
                     /locus_tag="10 aa linker"
                     /label="10 aa linker"
                     /ApEinfo_label="10 aa linker"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3079..3102
                     /label="NES"
                     /locus_tag="NES"
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2062..2103
                     /label="V5 tag"
                     /locus_tag="V5 tag"
                     /locus_tag="V5 tag"
                     /label="V5 tag"
                     /ApEinfo_label="V5 tag"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2458..2460
                     /label="R118S"
                     /locus_tag="R118S"
                     /locus_tag="R118S"
                     /label="R118S"
                     /ApEinfo_label="R118S"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2365..2367
                     /label="I87V"
                     /locus_tag="I87V"
                     /locus_tag="I87V"
                     /label="I87V"
                     /ApEinfo_label="I87V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3067..3077
                     /label="NES(1)"
                     /locus_tag="NES(1)"
                     /locus_tag="NES(1)"
                     /label="NES(1)"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2524..2526
                     /label="E141K"
                     /locus_tag="E141K"
                     /locus_tag="E141K"
                     /label="E141K"
                     /ApEinfo_label="E141K"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3078..3078
                     /label="NES(2)"
                     /locus_tag="NES(2)"
                     /locus_tag="NES(2)"
                     /label="NES(2)"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#a187be"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2449..2451
                     /label="G115G"
                     /locus_tag="G115G"
                     /locus_tag="G115G"
                     /label="G115G"
                     /ApEinfo_label="G115G"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_difference 2527..2529
                     /label="Q142R"
                     /locus_tag="Q142R"
                     /locus_tag="Q142R"
                     /label="Q142R"
                     /ApEinfo_label="Q142R"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2110..3066
                     /label="BirA (humanized)"
                     /locus_tag="BirA (humanized)"
                     /locus_tag="BirA (humanized)"
                     /label="BirA (humanized)"
                     /ApEinfo_label="BirA (humanized)"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2728..2730
                     /label="M209V"
                     /locus_tag="M209V"
                     /locus_tag="M209V"
                     /label="M209V"
                     /ApEinfo_label="M209V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2581..2583
                     /label="V160A"
                     /locus_tag="V160A"
                     /locus_tag="V160A"
                     /label="V160A"
                     /ApEinfo_label="V160A"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2677..2679
                     /label="T192A"
                     /locus_tag="T192A"
                     /locus_tag="T192A"
                     /label="T192A"
                     /ApEinfo_label="T192A"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_structure  2974..2976
                     /label="G291G"
                     /locus_tag="G291G"
                     /locus_tag="G291G"
                     /label="G291G"
                     /ApEinfo_label="G291G"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_difference 2683..2685
                     /label="K194I"
                     /locus_tag="K194I"
                     /locus_tag="K194I"
                     /label="K194I"
                     /ApEinfo_label="K194I"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3016..3018
                     /label="I305V"
                     /locus_tag="I305V"
                     /locus_tag="I305V"
                     /label="I305V"
                     /ApEinfo_label="I305V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2824..2826
                     /label="M241T"
                     /locus_tag="M241T"
                     /locus_tag="M241T"
                     /label="M241T"
                     /ApEinfo_label="M241T"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2104..2109
                     /label="NheI"
                     /locus_tag="NheI"
                     /locus_tag="NheI"
                     /label="NheI"
                     /ApEinfo_label="NheI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2554..2556
                     /label="L151P"
                     /locus_tag="L151P"
                     /locus_tag="L151P"
                     /label="L151P"
                     /ApEinfo_label="L151P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2299..2301
                     /label="Q65P"
                     /locus_tag="Q65P"
                     /locus_tag="Q65P"
                     /label="Q65P"
                     /ApEinfo_label="Q65P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2551..2553
                     /label="S150G"
                     /locus_tag="S150G"
                     /locus_tag="S150G"
                     /label="S150G"
                     /ApEinfo_label="S150G"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2890..2892
                     /label="S263P"
                     /locus_tag="S263P"
                     /locus_tag="S263P"
                     /label="S263P"
                     /ApEinfo_label="S263P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(3115..3136)
                     /PCR_conditions="primer sequence:GATCAGCGAGCTCTAGctcgag"
                     /note="22&#9;59.9&#9;59"
                     /locus_tag="RZ8-Gal4 R"
                     /label="RZ8-Gal4 R"
                     /ApEinfo_label="RZ8-Gal4 R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(3139..3156)
                     /PCR_conditions="primer sequence:TAGAAGGCACAGTCGAGG"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="BGHR"
                     /label="BGHR"
                     /ApEinfo_label="BGHR"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     617..656
                     /PCR_conditions="primer
                     sequence:ctgagagcgcagtcgagaagaagcttgccaccatggagac"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ62-syn-CD4"
                     /label="RZ62-syn-CD4"
                     /ApEinfo_label="RZ62-syn-CD4"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     567..588
                     /PCR_conditions="primer sequence:actcagcgctgcctcagtctgc"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ64-Syn-F Sequencing"
                     /label="RZ64-Syn-F Sequencing"
                     /ApEinfo_label="RZ64-Syn-F Sequencing"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     1046..1058
                     /PCR_conditions="primer sequence:actcagcgctgcctcagtctgc"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ64-Syn-F Sequencing(1)"
                     /label="RZ64-Syn-F Sequencing(1)"
                     /ApEinfo_label="RZ64-Syn-F Sequencing"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(2042..2061)
                     /PCR_conditions="primer sequence:gctaccagagccaccatgaa"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ91-CIBN-linker-R"
                     /label="RZ91-CIBN-linker-R"
                     /ApEinfo_label="RZ91-CIBN-linker-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     2042..2081
                     /PCR_conditions="primer
                     sequence:ttcatggtggctctggtagcGGCAAGCCCATCCCCAACCC"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ104-CIBN-Linker-V5-F"
                     /label="RZ104-CIBN-Linker-V5-F"
                     /ApEinfo_label="RZ104-CIBN-Linker-V5-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttgccaccat ggagacagac
      661 acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga cggggcccag
      721 ccggccagat ctTACCCATA CGATGTGCCA GATTACGCCt atccctatga cgtccctgat
      781 tacgctCTCG ACTTCCAGAA GGCCTCCAGC ATAGTCTATA AGAAAGAGGG GGAACAGGTG
      841 GAGTTCTCCT TCCCACTCGC CTTTACAGTT GAAAAGCTGA CGGGCAGTGG CGAGCTGTGG
      901 TGGCAGGCGG AGAGGGCTTC CTCCTCCAAG TCTTGGATCA CCTTTGACCT GAAGAACAAG
      961 GAAGTGTCTG TAAAACGGGT TACCCAGGAC CCTAAGCTCC AGATGGGCAA GAAGCTCCCG
     1021 CTCCACCTCA CCCTGCCCCA GGCCTTGCCT CAGTATGCTG GCTCTGGAAA CCTCACCCTG
     1081 GCCCTTGAAG CGAAAACAGG AAAGTTGCAT CAGGAAGTGA ACCTGGTGGT GATGAGAGCC
     1141 ACTCAGCTCC AGAAAAATTT GACCTGTGAG GTGTGGGGAC CCACCTCCCC TAAGCTGATG
     1201 CTGAGCTTGA AACTGGAGAA CAAGGAGGCA AAGGTCTCGA AGCGGGAGAA GGCGGTGTGG
     1261 GTGCTGAACC CTGAGGCGGG GATGTGGCAG TGTCTGCTGA GTGACTCGGG ACAGGTCCTG
     1321 CTGGAATCCA ACATCAAGGT TCTGCCCACA TGGTCCACCC CGGTGCAGCC AATGGCCCTG
     1381 ATTGTGCTGG GGGGCGTCGC CGGCCTCCTG CTTTTCATTG GGCTAGGCAT CTTCTTCTGT
     1441 GTCAGGTGCC GGCACCGAAG GCGCAAGGGC TCGGGCTCGA CCTCGGGCTC GGGCagcggt
     1501 ggcTCTAGAG GTTCTGGTGG CAGCTCTGGA GGTatgaatg gagctatagg aggtgacctt
     1561 ttgctcaatt ttcctgacat gtcggtccta gagcgccaaa gggctcacct caagtacctc
     1621 aatcccacct ttgattctcc tctcgccggc ttctttgccg attcttcaat gattaccggc
     1681 ggcgagatgg acagctatct ttcgactgcc ggtttgaatc ttccgatgat gtacggtgag
     1741 acgacggtgg aaggtgattc aagactctca atttcgccgg aaacgacgct tgggactgga
     1801 aatttcaagG CaGCgaagtt tgatacagag actaaggatt gtaatgagGC gGCgaagaag
     1861 atgacgatga acagagatga cctagtagaa gaaggagaag aagagaagtc gaaaataaca
     1921 gagcaaaaca atgggagcac aaaaagcatc aagaagatga aacacaaagc caagaaagaa
     1981 gagaacaatt tctctaatga ttcatctaaa gtgacgaagg aattggagaa aacggattat
     2041 attcatggtg gctctggtag cGGCAAGCCC ATCCCCAACC CCCTGCTGGG CCTGGACAGC
     2101 ACCGCTAGCA AAGACAATAC TGTGCCTCTG AAGCTGATCG CTCTCCTGGC TAATGGCGAG
     2161 TTCCATAGTG GCGAACAGCT GGGAGAAACC CTGGGCATGT CCAGGGCCGC TATCAACAAG
     2221 CACATTCAGA CTCTGCGCGA CTGGGGCGTG GACGTGTTCA CCGTGCCCGG AAAGGGCTAC
     2281 TCTCTGCCCG AGCCTATCCC GCTGCTGAAC GCTAAACAGA TTCTGGGACA GCTGGACGGC
     2341 GGGAGCGTGG CAGTCCTGCC TGTGGTCGAC TCCACCAATC AGTACCTGCT GGATCGAATC
     2401 GGCGAGCTGA AGAGTGGGGA TGCTTGCATT GCAGAATATC AGCAGGCAGG GAGAGGAAGC
     2461 AGAGGGAGGA AATGGTTCTC TCCTTTTGGA GCTAACCTGT ACCTGAGTAT GTTTTGGCGC
     2521 CTGAAGCGGG GACCAGCAGC AATCGGCCTG GGCCCGGTCA TCGGAATTGT CATGGCAGAA
     2581 GCGCTGCGAA AGCTGGGAGC AGACAAGGTG CGAGTCAAAT GGCCCAATGA CCTGTATCTG
     2641 CAGGATAGAA AGCTGGCAGG CATCCTGGTG GAGCTGGCCG GAATAACAGG CGATGCTGCA
     2701 CAGATCGTCA TTGGCGCCGG GATTAACGTG GCTATGAGGC GCGTGGAGGA AAGCGTGGTC
     2761 AATCAGGGCT GGATCACACT GCAGGAAGCA GGGATTAACC TGGACAGGAA TACTCTGGCC
     2821 GCTACGCTGA TCCGAGAGCT GCGGGCAGCC CTGGAACTGT TCGAGCAGGA AGGCCTGGCT
     2881 CCATATCTGC CACGGTGGGA GAAGCTGGAT AACTTCATCA ATAGACCCGT GAAGCTGATC
     2941 ATTGGGGACA AAGAGATTTT CGGGATTAGC CGGGGGATTG ATAAACAGGG AGCCCTGCTG
     3001 CTGGAACAGG ACGGAGTTAT CAAACCCTGG ATGGGCGGAG AAATCAGTCT GCGGTCTGCC
     3061 GAAAAGCTGC AGCTGCCTCC CCTGGAGCGC CTGACCCTGG ACTAATAGGA ATTCctcgag
     3121 CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC
     3181 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA
     3241 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
     3301 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GAggtaacca
     3361 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3421 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3481 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3541 tctccttacg catctgtgcg gtattcacac cgcatacgtc aaagcaacca tagtacgcgc
     3601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3781 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3901 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     3961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4021 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4081 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4201 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg
     5161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5581 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     5641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
