LOCUS       C69_pAAV_Syn_Nrx        5646 bp    DNA        circular     24-NOV-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     UUID: d2185ba0-9778-43af-afea-a1a483514b56
COMMENT     Type: Synthetic
COMMENT     ConfirmedExperimentally: 0
COMMENT     SequenceClass: UNA
COMMENT     TransformedInto: unspecified
COMMENT     Comments: Template 155A digested with BamHI,
            NheI&lt;br>f1:gaagcttGCCACCatgCACCTGAGAATCCACGCGAGACGG&lt;br>f2:gtc
            gtgcctgagagcgcagtcgagaagaagcttGCCACCatgCACCTGAGAATC&lt;br>r1:GTATGG
            CTCCGGCcAGCTTGCGCCGCGC&lt;br>f2:GCGCGGCGCAAGCTgGCCGGAGCCATAC&lt;br>
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="ITR"
                     /locus_tag="ITR"
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    151..628
                     /label="synapsin promoter"
                     /locus_tag="synapsin promoter"
                     /locus_tag="synapsin promoter"
                     /label="synapsin promoter"
                     /ApEinfo_label="synapsin promoter"
                     /ApEinfo_fwdcolor="#4eff51"
                     /ApEinfo_revcolor="#4eff51"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2910..3050
                     /label="ITR(1)"
                     /locus_tag="ITR(1)"
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2650..2881
                     /label="PolyA"
                     /locus_tag="PolyA"
                     /locus_tag="PolyA"
                     /label="PolyA"
                     /ApEinfo_label="PolyA"
                     /ApEinfo_fwdcolor="#ff2549"
                     /ApEinfo_revcolor="#ff2549"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2638..2643
                     /label="EcoRI"
                     /locus_tag="EcoRI"
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#46ff6e"
                     /ApEinfo_revcolor="#46ff6e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    643..648
                     /label="Kozak Sequence"
                     /locus_tag="Kozak Sequence"
                     /locus_tag="Kozak Sequence"
                     /label="Kozak Sequence"
                     /ApEinfo_label="Kozak Sequence"
                     /ApEinfo_fwdcolor="#00f900"
                     /ApEinfo_revcolor="#00f900"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    637..642
                     /label="HindIII"
                     /locus_tag="HindIII"
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#ff9200"
                     /ApEinfo_revcolor="#ff9200"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    649..651
                     /label="START Codon"
                     /locus_tag="START Codon"
                     /locus_tag="START Codon"
                     /label="START Codon"
                     /ApEinfo_label="START Codon"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(2632..2637)
                     /label="double stop"
                     /locus_tag="double stop"
                     /locus_tag="double stop"
                     /label="double stop"
                     /ApEinfo_label="double stop"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2608..2631
                     /label="NES"
                     /locus_tag="NES"
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1591..1632
                     /label="V5 tag"
                     /locus_tag="V5 tag"
                     /locus_tag="V5 tag"
                     /label="V5 tag"
                     /ApEinfo_label="V5 tag"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1987..1989
                     /label="R118S"
                     /locus_tag="R118S"
                     /locus_tag="R118S"
                     /label="R118S"
                     /ApEinfo_label="R118S"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1894..1896
                     /label="I87V"
                     /locus_tag="I87V"
                     /locus_tag="I87V"
                     /label="I87V"
                     /ApEinfo_label="I87V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2596..2606
                     /label="NES(1)"
                     /locus_tag="NES(1)"
                     /locus_tag="NES(1)"
                     /label="NES(1)"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2053..2055
                     /label="E141K"
                     /locus_tag="E141K"
                     /locus_tag="E141K"
                     /label="E141K"
                     /ApEinfo_label="E141K"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2607..2607
                     /label="NES(2)"
                     /locus_tag="NES(2)"
                     /locus_tag="NES(2)"
                     /label="NES(2)"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#a187be"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1978..1980
                     /label="G115G"
                     /locus_tag="G115G"
                     /locus_tag="G115G"
                     /label="G115G"
                     /ApEinfo_label="G115G"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_difference 2056..2058
                     /label="Q142R"
                     /locus_tag="Q142R"
                     /locus_tag="Q142R"
                     /label="Q142R"
                     /ApEinfo_label="Q142R"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1639..2595
                     /label="BirA (humanized)"
                     /locus_tag="BirA (humanized)"
                     /locus_tag="BirA (humanized)"
                     /label="BirA (humanized)"
                     /ApEinfo_label="BirA (humanized)"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2257..2259
                     /label="M209V"
                     /locus_tag="M209V"
                     /locus_tag="M209V"
                     /label="M209V"
                     /ApEinfo_label="M209V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2110..2112
                     /label="V160A"
                     /locus_tag="V160A"
                     /locus_tag="V160A"
                     /label="V160A"
                     /ApEinfo_label="V160A"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2206..2208
                     /label="T192A"
                     /locus_tag="T192A"
                     /locus_tag="T192A"
                     /label="T192A"
                     /ApEinfo_label="T192A"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_structure  2503..2505
                     /label="G291G"
                     /locus_tag="G291G"
                     /locus_tag="G291G"
                     /label="G291G"
                     /ApEinfo_label="G291G"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_difference 2212..2214
                     /label="K194I"
                     /locus_tag="K194I"
                     /locus_tag="K194I"
                     /label="K194I"
                     /ApEinfo_label="K194I"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2545..2547
                     /label="I305V"
                     /locus_tag="I305V"
                     /locus_tag="I305V"
                     /label="I305V"
                     /ApEinfo_label="I305V"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2353..2355
                     /label="M241T"
                     /locus_tag="M241T"
                     /locus_tag="M241T"
                     /label="M241T"
                     /ApEinfo_label="M241T"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1633..1638
                     /label="NheI"
                     /locus_tag="NheI"
                     /locus_tag="NheI"
                     /label="NheI"
                     /ApEinfo_label="NheI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2083..2085
                     /label="L151P"
                     /locus_tag="L151P"
                     /locus_tag="L151P"
                     /label="L151P"
                     /ApEinfo_label="L151P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1828..1830
                     /label="Q65P"
                     /locus_tag="Q65P"
                     /locus_tag="Q65P"
                     /label="Q65P"
                     /ApEinfo_label="Q65P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2080..2082
                     /label="S150G"
                     /locus_tag="S150G"
                     /locus_tag="S150G"
                     /label="S150G"
                     /ApEinfo_label="S150G"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2419..2421
                     /label="S263P"
                     /locus_tag="S263P"
                     /locus_tag="S263P"
                     /label="S263P"
                     /ApEinfo_label="S263P"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    811..837
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="#cc99b2"
                     /ApEinfo_revcolor="#cc99b2"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    652..996
                     /locus_tag="Nrxn3b"
                     /label="Nrxn3b"
                     /ApEinfo_label="Nrxn3b"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    997..1062
                     /locus_tag="Linker"
                     /label="Linker"
                     /ApEinfo_label="Linker"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1063..1575
                     /locus_tag="CIBN"
                     /label="CIBN"
                     /ApEinfo_label="CIBN"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1576..1590
                     /locus_tag="Linker(1)"
                     /label="Linker(1)"
                     /ApEinfo_label="Linker"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(2644..2665)
                     /PCR_conditions="primer sequence:GATCAGCGAGCTCTAGctcgag"
                     /note="22&#9;59.9&#9;59"
                     /locus_tag="RZ8-Gal4 R"
                     /label="RZ8-Gal4 R"
                     /ApEinfo_label="RZ8-Gal4 R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(2668..2685)
                     /PCR_conditions="primer sequence:TAGAAGGCACAGTCGAGG"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="BGHR"
                     /label="BGHR"
                     /ApEinfo_label="BGHR"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     567..588
                     /PCR_conditions="primer sequence:actcagcgctgcctcagtctgc"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ64-Syn-F Sequencing"
                     /label="RZ64-Syn-F Sequencing"
                     /ApEinfo_label="RZ64-Syn-F Sequencing"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     1571..1610
                     /PCR_conditions="primer
                     sequence:ttcatggtggctctggtagcGGCAAGCCCATCCCCAACCC"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ104-CIBN-Linker-V5-F"
                     /label="RZ104-CIBN-Linker-V5-F"
                     /ApEinfo_label="RZ104-CIBN-Linker-V5-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(1571..1590)
                     /PCR_conditions="primer sequence:gctaccagagccaccatgaa"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ91-CIBN-linker-R"
                     /label="RZ91-CIBN-linker-R"
                     /ApEinfo_label="RZ91-CIBN-linker-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     617..656
                     /PCR_conditions="primer
                     sequence:ctgagagcgcagtcgagaagaagcttgccaccatgcacct"
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
                     /locus_tag="RZ69-syn-NRX3b-F"
                     /label="RZ69-syn-NRX3b-F"
                     /ApEinfo_label="RZ69-syn-NRX3b-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttgccaccat gcacctgaga
      661 atccacgcga gacggagccc tcctcgccgg ccggcctgga cgcttgggat ctggttcctg
      721 ttctggggat gtatcgtcag ctctgtatgg agtcaattgt cttctaatgt agcttcctcc
      781 tcctccacct cttcctcgcc ggggtctcac tacccatacg atgtgccaga ttacgccaac
      841 cccacggagc cgggaatcag acgggttccg ggggcctcag aggtgatccg ggagtcgagc
      901 agcacaacag ggatggtcgt cggcattgtg gctgctgccg ccctctgcat cttgatcctc
      961 ctgtacgcca tgtacaagta caggaacagg gacgagggct cgggctcgac ctcgggctcg
     1021 ggcagcggtg gctctggagg ttctggtggc agctctggag gtatgaatgg agctatagga
     1081 ggtgaccttt tgctcaattt tcctgacatg tcggtcctag agcgccaaag ggctcacctc
     1141 aagtacctca atcccacctt tgattctcct ctcgccggct tctttgccga ttcttcaatg
     1201 attaccggcg gcgagatgga cagctatctt tcgactgccg gtttgaatct tccgatgatg
     1261 tacggtgaga cgacggtgga aggtgattca agactctcaa tttcgccgga aacgacgctt
     1321 gggactggaa atttcaaggc agcgaagttt gatacagaga ctaaggattg taatgaggcg
     1381 gcgaagaaga tgacgatgaa cagagatgac ctagtagaag aaggagaaga agagaagtcg
     1441 aaaataacag agcaaaacaa tgggagcaca aaaagcatca agaagatgaa acacaaagcc
     1501 aagaaagaag agaacaattt ctctaatgat tcatctaaag tgacgaagga attggagaaa
     1561 acggattata ttcatggtgg ctctggtagc GGCAAGCCCA TCCCCAACCC CCTGCTGGGC
     1621 CTGGACAGCA CCGCTAGCAA AGACAATACT GTGCCTCTGA AGCTGATCGC TCTCCTGGCT
     1681 AATGGCGAGT TCCATAGTGG CGAACAGCTG GGAGAAACCC TGGGCATGTC CAGGGCCGCT
     1741 ATCAACAAGC ACATTCAGAC TCTGCGCGAC TGGGGCGTGG ACGTGTTCAC CGTGCCCGGA
     1801 AAGGGCTACT CTCTGCCCGA GCCTATCCCG CTGCTGAACG CTAAACAGAT TCTGGGACAG
     1861 CTGGACGGCG GGAGCGTGGC AGTCCTGCCT GTGGTCGACT CCACCAATCA GTACCTGCTG
     1921 GATCGAATCG GCGAGCTGAA GAGTGGGGAT GCTTGCATTG CAGAATATCA GCAGGCAGGG
     1981 AGAGGAAGCA GAGGGAGGAA ATGGTTCTCT CCTTTTGGAG CTAACCTGTA CCTGAGTATG
     2041 TTTTGGCGCC TGAAGCGGGG ACCAGCAGCA ATCGGCCTGG GCCCGGTCAT CGGAATTGTC
     2101 ATGGCAGAAG CGCTGCGAAA GCTGGGAGCA GACAAGGTGC GAGTCAAATG GCCCAATGAC
     2161 CTGTATCTGC AGGATAGAAA GCTGGCAGGC ATCCTGGTGG AGCTGGCCGG AATAACAGGC
     2221 GATGCTGCAC AGATCGTCAT TGGCGCCGGG ATTAACGTGG CTATGAGGCG CGTGGAGGAA
     2281 AGCGTGGTCA ATCAGGGCTG GATCACACTG CAGGAAGCAG GGATTAACCT GGACAGGAAT
     2341 ACTCTGGCCG CTACGCTGAT CCGAGAGCTG CGGGCAGCCC TGGAACTGTT CGAGCAGGAA
     2401 GGCCTGGCTC CATATCTGCC ACGGTGGGAG AAGCTGGATA ACTTCATCAA TAGACCCGTG
     2461 AAGCTGATCA TTGGGGACAA AGAGATTTTC GGGATTAGCC GGGGGATTGA TAAACAGGGA
     2521 GCCCTGCTGC TGGAACAGGA CGGAGTTATC AAACCCTGGA TGGGCGGAGA AATCAGTCTG
     2581 CGGTCTGCCG AAAAGCTGCA GCTGCCTCCC CTGGAGCGCC TGACCCTGGA CTAATAGGAA
     2641 TTCctcgagC TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG
     2701 TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT
     2761 CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG
     2821 GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG
     2881 Aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     2941 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3001 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3061 gcggtatttt ctccttacgc atctgtgcgg tattcacacc gcatacgtca aagcaaccat
     3121 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3181 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3241 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3301 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3361 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3421 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     3481 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3541 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4681 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5641 acatgt     
//
