LOCUS       Exported                7687 bp ds-DNA     circular SYN 07-11-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn1-DIO-optoFGFR1-HA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Bio-imaging
  TITLE     Direct Submission
  JOURNAL   Exported 2025¤¤¤Å¤¤ 11 25¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    2..142
                     /note="ITR"
     promoter        157..604
                     /note="hSyn promoter"
     protein_bind    623..656
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    707..740
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(762..788)
                     /codon_start=1
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             complement(810..2303)
                     /codon_start=1
                     /note="PHR'"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGA
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(2311..3593)
                     /note="FGFR1"
     CDS             complement(3600..3665)
                     /codon_start=1
                     /note="Lyn signal peptide"
                     /translation="MGCIKSKGKDSAGADSAGSAGS"
     protein_bind    complement(3673..3706)
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(3757..3790)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3815..4403
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4286..4297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     polyA_signal    4435..4911
                     /note="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     rep_origin      5166..5621
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5903..6007
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6008..6868
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7039..7627
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc ctgcggccgc acgcgtagtg caagtgggtt ttaggaccag
      181 gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc
      241 aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg
      301 gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg
      361 cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc
      421 gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc
      481 gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg
      541 cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc
      601 gcagtctaga gtcgactccg gaataacttc gtataggata ctttatacga agttatgcag
      661 aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa cttcgtatag
      721 catacattat acgaagttat gggcggccgc ggcgcgcctt aagcataatc tggaacatca
      781 tatggatata ccggtgggct gccgctgccg gcagcaccga tcataatctg cgcctcccga
      841 gtgcgagaga tcgctttagc cagcagctcg cgtgctgtat ctatatcaac tataggctta
      901 gcgtaattgg tgcccagctc caccccgctt gccttgagca ctgtcagtgg tgcgtcccaa
      961 ggatggtgaa tccactccgt aggaagtcgg gccagctcgg ggagccactg tcgaatatac
     1021 tctccttcgg gatcatactt agctccctgc aaggccggat tgtccaacct gtccaactca
     1081 tggccgtcag gaattgaccc gctaatatat tgccatccca gaatgtcaca ctccaagtcg
     1141 gcgtcgagaa gggtatccca aaagtacttc attccccatt tccaaggcag gagcagaaac
     1201 ttgacagcaa atgaggacac gattacccgg atgcggttgt gcatccagcc tgtagcccag
     1261 agctctctca tgccagcatc cacgagtggg taccctgtcc ttccctgtct ccatgccttg
     1321 aatttgtcga catcggcgtc ccaggggaag aacctgaggt gggacaacag actctgctcg
     1381 tgtgtgaatg gaaagttgaa acatatatac cgggagtatt cccgaagtcc gatccctcgc
     1441 aggaacagat cggcgctttc ggcgccttcg ctgtttttat cgcgagccca gataatctgt
     1501 ttcatccgag cgcactgaaa tacatggcgg acagaaatct cgccaaaatg gagatatgga
     1561 gagagcaagc tagttgagtt acctaccact ttcttggagt tcttcgcgta gtcaatcagt
     1621 tgtttttcga tgaactcgtt aagcagcttg tcggcattag accagccggg gctccaggct
     1681 ctagtgagaa gtgcattgct tggcttttct gcttcatttt ccagaccgag ttcctcgatg
     1741 gagcaggccc agatggcctc tgcagcagca gtaatcggca tcagtctcca gggagggggc
     1801 aacataacgg actctattga catatccaaa catttcttcc agtaactgtt gaaggaggtg
     1861 aacggtttcc ctttctcgca atagatctcc catggctcgt acagaaggtc cccgttgtag
     1921 ctctgcacac tgatcccccg ttccaccagc ttctccttca cagtgtggtc gcgcaccaga
     1981 ctaactggat cgtacaggtg gttaaacacg actttcgttg cgccggtcac cctgatgcag
     2041 tcgaggatgg cagagatggt attgtgtgtc ttgatcaggg taagatcact gcccagtgct
     2101 ttcagtgact ggctaagatg ggccagactt tgcttcatcc accacctaga ggcccttccg
     2161 ggataaaact ggccttcttc ttcaggacac caaatgaaaa cggggaagac ggaaccctcg
     2221 tgggccgcgg cggcgagcgc gggattatct tctattctca aatctctccg aaaccagacg
     2281 atggtctttt tgtccatctt catgggatcc gcgcggcgtt tgagtccgcc attggcaagc
     2341 tgggctgggt gtcggggcag gcagggctcc tcgggcagcg gctcatgaga gaagacggaa
     2401 tcctcccctg aggagcacgt agagctccgg gtgtcgggaa agctggggga gtactggtcc
     2461 aggggcatgg acaggtccag gtactcctgg ttggaggtca aggccacgat gcggtccagg
     2521 tcttccacca gctgcttgaa ggtgggtctc tgtgagggca ctgcatgcca gcagtcccgc
     2581 atcatcatgt acagctcgtt ggtgcagtta ctgggcttgt ccatgcggtg accctccttc
     2641 agcagcttga aaagttcctc cacaggcaca ccggggtatg gggagccgcc cagagtgaag
     2701 atctcccaca ggagcacccc gaaagaccac acatcactct ggtgggtgta gatccggtca
     2761 aataatgcct cgggtgccat ccacttcaca ggcagtcggc cgttggttgt ctttttatag
     2821 tagtcgatgt ggtgaatgtc ccgtgcgagg ccaaagtctg ctatcttcat cacattgtcc
     2881 tctgtcacca ggacattcct ggctgccagg tctcggtgta tgcacttctt ggaggccaga
     2941 tactccatgc ctcgggccac ctggtaggcg caggacacca ggtccttgga ggagagctgc
     3001 tcctctgggt tgtggctggg gttgtagcag tattccagcc ctgggggcct ccgggcctgc
     3061 aggtactccc gcaggttgcc cttggaggca tactccacga tgacatacaa gggaccatcc
     3121 tgcgtgcagg cccccagcag gttgatgata ttcttatgct tcccgatcat cttcatcatc
     3181 tccatttctg agatcaggtc tgacaagtct ttctctgttg cgtccgactt caacatcttc
     3241 acagccactt tggtcacacg gttgggtttg tccttgtcca gcccgatagc ctctgccaac
     3301 accacctgcc caaagcagcc ctctcccagg ggtttgccta agaccagtct gtcccgaggc
     3361 agctcccagc gagggtcttc gggaagctca tactcagaga cccctgctag catgggagtc
     3421 ccactggagg agagccgtga tggccgaacc agaagaaccc cagagttcat ggatgcactg
     3481 gagtcagcag acactgttac ctgtctgcgc agagggatgc tcttggccag cttgtgcaca
     3541 gccatctggc tgtggaagtc actcttcttg gtaccactct tcatcttgga attcccagca
     3601 ctaccagcac taccagcact atctgctccc gcgctgtctt tcccttttga ttttatacat
     3661 cccatggtgg ccataacttc gtataaagta tcctatacga agttatttgc cttaacccag
     3721 aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat
     3781 acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4201 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4381 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     4441 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4501 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4561 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4621 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4681 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     4741 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     4801 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4861 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     4921 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4981 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     5041 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     5101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     5161 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     5521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5581 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5641 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5701 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5761 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5821 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5881 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5941 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6001 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6061 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6241 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6361 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6541 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7141 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7321 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7381 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7441 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7501 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7561 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7621 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7681 tcacatg
//
