LOCUS       Exported                7367 bp ds-DNA     circular SYN 19-12-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CamKII0.4-optoFGFR1-HA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Bio-imaging
  TITLE     Direct Submission
  JOURNAL   Exported 2025¤¤¤Å¤¤ 11 25¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        157..520
                     /note="CamKIIa promoter"
     CDS             557..622
                     /codon_start=1
                     /note="Lyn signal peptide"
                     /translation="MGCIKSKGKDSAGADSAGSAGS"
     CDS             629..1911
                     /note="FGFR1"
     CDS             1919..3412
                     /codon_start=1
                     /note="PHR'"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGA
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3434..3460
                     /codon_start=1
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    3496..4084
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3967..3978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(4087..4103)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4114..4590
                     /note="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4630..4770
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4845..5300
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5582..5686
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             5687..6547
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6718..7306
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaactt gtggactaag tttgttcaca
      181 tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac ttgctcctgg
      241 gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag caggttttaa
      301 agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac agggcgtttc
      361 ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg tgagcagcca
      421 cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt ttgcactcag
      481 gagcacgggc aggcgagtgg cccctagttc tgggggcagc tctagagccg atccccgggt
      541 accggtgcta gccaccatgg gatgtataaa atcaaaaggg aaagacagcg cgggagcaga
      601 tagtgctggt agtgctggta gtgctgggaa ttccaagatg aagagtggta ccaagaagag
      661 tgacttccac agccagatgg ctgtgcacaa gctggccaag agcatccctc tgcgcagaca
      721 ggtaacagtg tctgctgact ccagtgcatc catgaactct ggggttcttc tggttcggcc
      781 atcacggctc tcctccagtg ggactcccat gctagcaggg gtctctgagt atgagcttcc
      841 cgaagaccct cgctgggagc tgcctcggga cagactggtc ttaggcaaac ccctgggaga
      901 gggctgcttt gggcaggtgg tgttggcaga ggctatcggg ctggacaagg acaaacccaa
      961 ccgtgtgacc aaagtggctg tgaagatgtt gaagtcggac gcaacagaga aagacttgtc
     1021 agacctgatc tcagaaatgg agatgatgaa gatgatcggg aagcataaga atatcatcaa
     1081 cctgctgggg gcctgcacgc aggatggtcc cttgtatgtc atcgtggagt atgcctccaa
     1141 gggcaacctg cgggagtacc tgcaggcccg gaggccccca gggctggaat actgctacaa
     1201 ccccagccac aacccagagg agcagctctc ctccaaggac ctggtgtcct gcgcctacca
     1261 ggtggcccga ggcatggagt atctggcctc caagaagtgc atacaccgag acctggcagc
     1321 caggaatgtc ctggtgacag aggacaatgt gatgaagata gcagactttg gcctcgcacg
     1381 ggacattcac cacatcgact actataaaaa gacaaccaac ggccgactgc ctgtgaagtg
     1441 gatggcaccc gaggcattat ttgaccggat ctacacccac cagagtgatg tgtggtcttt
     1501 cggggtgctc ctgtgggaga tcttcactct gggcggctcc ccataccccg gtgtgcctgt
     1561 ggaggaactt ttcaagctgc tgaaggaggg tcaccgcatg gacaagccca gtaactgcac
     1621 caacgagctg tacatgatga tgcgggactg ctggcatgca gtgccctcac agagacccac
     1681 cttcaagcag ctggtggaag acctggaccg catcgtggcc ttgacctcca accaggagta
     1741 cctggacctg tccatgcccc tggaccagta ctcccccagc tttcccgaca cccggagctc
     1801 tacgtgctcc tcaggggagg attccgtctt ctctcatgag ccgctgcccg aggagccctg
     1861 cctgccccga cacccagccc agcttgccaa tggcggactc aaacgccgcg cggatcccat
     1921 gaagatggac aaaaagacca tcgtctggtt tcggagagat ttgagaatag aagataatcc
     1981 cgcgctcgcc gccgcggccc acgagggttc cgtcttcccc gttttcattt ggtgtcctga
     2041 agaagaaggc cagttttatc ccggaagggc ctctaggtgg tggatgaagc aaagtctggc
     2101 ccatcttagc cagtcactga aagcactggg cagtgatctt accctgatca agacacacaa
     2161 taccatctct gccatcctcg actgcatcag ggtgaccggc gcaacgaaag tcgtgtttaa
     2221 ccacctgtac gatccagtta gtctggtgcg cgaccacact gtgaaggaga agctggtgga
     2281 acgggggatc agtgtgcaga gctacaacgg ggaccttctg tacgagccat gggagatcta
     2341 ttgcgagaaa gggaaaccgt tcacctcctt caacagttac tggaagaaat gtttggatat
     2401 gtcaatagag tccgttatgt tgccccctcc ctggagactg atgccgatta ctgctgctgc
     2461 agaggccatc tgggcctgct ccatcgagga actcggtctg gaaaatgaag cagaaaagcc
     2521 aagcaatgca cttctcacta gagcctggag ccccggctgg tctaatgccg acaagctgct
     2581 taacgagttc atcgaaaaac aactgattga ctacgcgaag aactccaaga aagtggtagg
     2641 taactcaact agcttgctct ctccatatct ccattttggc gagatttctg tccgccatgt
     2701 atttcagtgc gctcggatga aacagattat ctgggctcgc gataaaaaca gcgaaggcgc
     2761 cgaaagcgcc gatctgttcc tgcgagggat cggacttcgg gaatactccc ggtatatatg
     2821 tttcaacttt ccattcacac acgagcagag tctgttgtcc cacctcaggt tcttcccctg
     2881 ggacgccgat gtcgacaaat tcaaggcatg gagacaggga aggacagggt acccactcgt
     2941 ggatgctggc atgagagagc tctgggctac aggctggatg cacaaccgca tccgggtaat
     3001 cgtgtcctca tttgctgtca agtttctgct cctgccttgg aaatggggaa tgaagtactt
     3061 ttgggatacc cttctcgacg ccgacttgga gtgtgacatt ctgggatggc aatatattag
     3121 cgggtcaatt cctgacggcc atgagttgga caggttggac aatccggcct tgcagggagc
     3181 taagtatgat cccgaaggag agtatattcg acagtggctc cccgagctgg cccgacttcc
     3241 tacggagtgg attcaccatc cttgggacgc accactgaca gtgctcaagg caagcggggt
     3301 ggagctgggc accaattacg ctaagcctat agttgatata gatacagcac gcgagctgct
     3361 ggctaaagcg atctctcgca ctcgggaggc gcagattatg atcggtgctg ccggcagcgg
     3421 cagcccaccg gtatatccat atgatgttcc agattatgct taaggcgcgc cgaattcgat
     3481 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3541 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3601 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3661 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3721 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3781 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3841 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3901 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3961 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4021 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4081 ccgcatcgat accgtcgacc tcgagagatc tacgggtggc atccctgtga cccctcccca
     4141 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     4201 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     4261 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     4321 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     4381 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     4441 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4501 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4561 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
     4621 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4681 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4741 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4801 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4861 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4921 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4981 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5041 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5101 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5161 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     5221 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5281 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5341 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5401 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5461 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5521 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5581 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5641 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5701 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5761 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5821 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5881 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5941 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6001 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6061 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6121 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6181 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6241 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6301 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6361 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6421 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6481 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6541 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6601 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6661 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6721 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6781 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6841 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6901 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6961 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7021 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7081 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7141 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7201 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7261 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7321 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
