LOCUS       Exported                5991 bp ds-DNA     circular SYN 24-NOV-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5991)
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported 20-JAN-2020 from NEBuilder https://nebuilder.neb.com
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase = 
            overlap) Ser_mEGFP_fwd: 
            aactttaagaaggagatataccatgtccatggtgAGCAAGGGCGAGGAGCTG Ser_mEGFP_fwd 
            3'Tm: 71.0  3'Ta: 69.3 Ser_mEGFP_rev: 
            agtggtggtggtggtggtgctcgagttaaacatgatggtggtgatgatgaccgccggtttccggcag 
            gccaccgctCTTGTACAGCTCGTCCATGCC Ser_mEGFP_rev 3'Tm: 68.3  3'Ta: 69.3.
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=pUC ori
                     /note="/created_by=matthiasmuhar"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #ffff00"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=old MFM16;
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     misc_feature    824^825
                     /label=TAT
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=TAT"
                     /note="color: #a6acb3"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop; rop"
                     /product="Rop protein, which maintains
                     plasmids at low copy number; Rop protein, which maintains 
                     plasmids at low copy number"
                     /label=rop
                     /note="/Transferred_From=pET28b-sfGFP-SGG-LPETGG_His6V 
                     (pMFM002)
                     translation, TOBECLONED 
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pET29b-IGIP1490, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MTKQEKTALNMARFIRSQTLTLLEKLN
                     ELDAD EQADICESLHDHADELYRSCLARFGDDGENL; 
                     VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGD 
                     DGENL*"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002) translation; 
                     pET28b_RH_ISG15_GyrA; pET29b-IGIP1490; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MTKQEKTALNMARFIRSQTLTLLEK
                     LNELD ADEQADICESLHDHADELYRSCLARFGDDGENL; 
                     VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGD 
                     DGENL*"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
                     /note="color: #993366"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI; lacI"
                     /product="lac repressor;
                     lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; The lac 
                     repressor binds to the lac operator to  inhibit 
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pJC1973 - pDZ2087 - 
                     tac-MBP-TEV_site-His6-TEV_protease(L56V/S235G/S219V), 
                     pMFM002 pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MKPVTLYDVAEYAGVSYQTVSRVVNQA
                     SHVSA 
                     KTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQL 
                     GASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFL 
                     DVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLT 
                     RNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRV 
                     GADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS 
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ; 
                     VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAG 
                     KQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLA 
                     QRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHL 
                     VALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQM 
                     LNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIK 
                     QDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQ 
                     LARQVSRLESGQ*"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=VKPVTLYDVAEYAGVSYQTVSRVVN
                     QASHV 
                     SAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRAD 
                     QLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPAL 
                     FLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY 
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGL 
                     RVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLP 
                     VSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /note="color: #993366"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3099..3176)
                     /gene="lacI; lacI"
                     /label=lacI promoter
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pET29b-IGIP1490, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pET29b-IGIP1490; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffffff; direction: LEFT"
     gene            complement(3190..3453)
                     /gene="tet (300 - 563)"
                     /label=tet (300 - 563)
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCAL 
                     CLLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDLLFACTGS 
                     LWLDQI*"
                     /note="/Transferred_From=IGI-P0165, IGI-P0165_inmod, 
                     IGI-P0213,
                     IGI-P0222 (pHR-hEF1a-dCas9-p300 core-IRES-GFP), IGI-P0222 
                     (pHR-hEF1a-dCas9-p300 core-IRES-GFP) Copy, IGI-P0222 mod 
                     ATP6V0A1, IGI-P0222 mod ATP6V0A1 Copy, IGI-P0222 mod 
                     ATP6V0A2, IGI-P0222 mod for cloning, TOBECLONED pMFM021 
                     pLenti-TRE3G-SpCas9-PGK-Neo (T3CpN) 33333, 
                     TOBECLONED_FINAL_pHR-SFFV-rtTA3-mPGK-BSD, 
                     TPOBECLONED_FINAL_pHR-SFFV-rtTA3-IRES-EcoRec-mPGK-BSD, 
                     atpv0a2 cDNA cloned into IGI-P0222 opened with MluI BsiWI, 
                     pSPN-001 (pHR-IRES-GFP), pSPN-002 (pHR-IRES-GFP ATG-free)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MLLLPVWLSFSPPCINPGCCLFMRSCG
                     PLSGN 
                     VAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALT 
                     IPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAF 
                     ALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ 
                     RRQDLLFACTGSLWLDQI*"
                     /note="/X-Transferred_From=IGI-P0165; IGI-P0165_inmod; 
                     TOBECLONED_FINAL_pHR-SFFV-rtTA3-mPGK-BSD; 
                     TPOBECLONED_FINAL_pHR-SFFV-rtTA3-IRES-EcoRec-mPGK-BSD"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MLLLPVWLSFSPPCINPGCCLFMRS
                     CGPLS 
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA 
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF 
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH 
                     SQRRQDLLFACTGSLWLDQI*"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        3485..3503
                     /label=T7 prom
                     
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #ffffff"
     misc_binding    3504..3528
                     /label=Lac O1
                     /note="/Transferred_From=IGI-P0062"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #800080"
     RBS             3543..3565
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     CDS             3572..4282
                     /label=mTagBFP2 (E coli K12 optimized)
                     /note="/created_by=matthiasmuhar"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4292..4309
                     /label=Srt recognition site
                     /note="/Transferred_From=X032-M16, 
                     pET28b-sfGFP-SGG-LPETGG_His6V
                     (pMFM002) - ORF translation, pET28b-sfGFP-SGG-LPETGG_His6V 
                     (pMFM002) translation, pET28b-sfGFP-SGG-LPETGG_His6V Copy -
                     ORF translation, 
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 translation, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002) - ORF translation; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002) translation; 
                     pET28b-sfGFP-SGG-LPETGG_His6V Copy - ORF translation; 
                     pMFM002 pET28b-sfGFP-SGG-LPETGG_His6V"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    4310..4327
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=X032-M16,
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 translation, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, pMFM016 
                     mod, pMFM016 mod Copy, 
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/codon_start=1"
                     /note="/modified_by=matthiasmuhar"
                     /note="/transl_table=1"
                     /note="color: #a6acb3"
     terminator      4424..4471
                     /label=T7 terminator
                     /note="transcription terminator for
                     bacteriophage T7 RNA polymerase"
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     rep_origin      4508..4963
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication;
                     arrow  indicates direction of (+) strand synthesis; arrow 
                     indicates direction of (+) strand synthesis; f1 
                     bacteriophage origin of replication"
                     /note="/Transferred_From=PAX8 AID N Tag Linker (1269),
                     TOBECLONED pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD 
                     Copy, TOBECLONED 
                     pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, TOBECLONED pMFM
                     pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), pET29b-IGIP1490, 
                     pMFM002 pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); pET29b-IGIP1490; 
                     pMFM002 pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffff00"
     misc_feature    4954
                     /label=T
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=T"
                     /note="color: #a6acb3"
     CDS             complement(5055..5870)
                     /codon_start=1
                     /gene="aph(3')-Ia;
                     aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase; aminoglycoside
                     phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in
                     bacteria or G418 (Geneticin(R)) in eukaryotes; confers 
                     resistance to kanamycin in bacteria or G418 (Geneticin(R)) 
                     in eukaryotes"
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pET29b-IGIP1490, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD, pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK, pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, pMFM013 
                     pET28b-His6-TEV(G)-mCherry, pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6, pMFM016 mod, pMFM016 mod 
                     Copy, pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6, 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3, pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD, pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK, pMFM020 
                     pET28b-His6-TEV(S)-mCherry, pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA, pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3 RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 RxxG, pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A, R2S, A206K)-ASCC3 DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MSHIQRETSCSRPRLNSNMDADLYGYK
                     WARDN 
                     VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRT 
                     PDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFR 
                     LAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIF 
                     DEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFH 
                     LMLDEFF; 
                     MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGK 
                     GSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDS 
                     GENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWP 
                     VEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAIL 
                     WNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /note="/X-Transferred_From=TOBECLONED_pMFM016_pET28b-Ser-sf
                     GFP(A 206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pET29b-IGIP1490; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MSHIQRETSCSRPRLNSNMDADLYG
                     YKWAR 
                     DNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFI 
                     RTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRV 
                     FRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNL 
                     IFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQ 
                     FHLMLDEFF; 
                     MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGK 
                     GSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDS 
                     GENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWP 
                     VEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAIL 
                     WNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /note="color: #993366"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     3541 attttgttta actttaagaa ggagatatac catggtctcc aagggcgaag aattgatcaa
     3601 ggagaatatg catatgaaac tgtatatgga gggtacagta gacaatcacc acttcaagtg
     3661 tacctcggag ggggaaggta agccatatga ggggactcaa acaatgcgta ttaaggtggt
     3721 tgagggtggt ccccttcctt tcgctttcga tattttggct acctcgttcc tttacggctc
     3781 aaagacgttt atcaatcaca cccaggggat tccagatttt tttaaacaat ccttccccga
     3841 ggggttcaca tgggaacgcg taacaactta tgaggatgga ggagtcttaa ccgccaccca
     3901 ggatactagc ttgcaagatg gctgtttgat ttacaatgta aaaatccgtg gtgttaattt
     3961 cacatcaaac ggtcctgtta tgcaaaagaa aactttaggg tgggaagcct tcacggagac
     4021 gctgtatccg gcggacgggg gcctggaggg acgtaatgac atggccctta aattggttgg
     4081 tggttcgcat ttgatcgcca acgccaaaac gacctatcgt tcaaagaagc ctgccaagaa
     4141 cttaaagatg cccggtgtat actacgttga ctatcgcctt gaacgtatca aggaagccaa
     4201 caatgagacc tatgtcgagc agcatgaggt cgccgtcgct cgctattgtg atctgccttc
     4261 caagttgggc cataaactta acagcggtgg cctgccggaa accggcggtc atcatcacca
     4321 ccatcatgtt taactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
     4381 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4441 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
     4501 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4561 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4801 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4861 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4921 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5041 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     5101 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     5161 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     5221 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     5281 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     5341 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     5401 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     5461 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     5521 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     5581 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     5641 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     5701 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     5761 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     5821 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     5881 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga
     5941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
