LOCUS       Exported                7641 bp ds-DNA     circular UNA 24-NOV-2025
DEFINITION  .
ACCESSION   urn.local...oc-jxr8ydk
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /note="color: #ffff00"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     misc_feature    824^825
                     /label=TAT
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=TAT"
                     /note="color: #a6acb3"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains
                     plasmids at low copy number"
                     /label=rop
                     /note="color: #993366"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        3485..3503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     protein_bind    3504..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             3543..3565
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             3573..4022
                     /label=ddSKP1 (E coli K12 codon optimized)
                     /note="/created_by=matthiasmuhar"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4076..4092
                     /gene="T7"
                     /label=T7 Promoter
                     /note="Geneious type: promoter prokaryotic"
                     /note="/Transferred_From=TOBECLONED
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG, TOBECLONED pMFM0xx 
                     pMT2-SKP1dd-StrepTagII-truncFBXO31, pHYRSF53, pMFM040 
                     pMT2-His6-Smt3-StrepTagII-FBXO31.1, temp 3XFLAG-FBXO31"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG; pHYRSF53"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     protein_bind    4095..4119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, 
                     pMFM0xx_H6-3xHA-sfGFP-DxxD"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=A206K)-ASCC3_DxxD; TOBECLONED 
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy; 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD; 
                     pMFM002 pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     RBS             4132..4154
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="/Transferred_From=TOBECLONED
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy, 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD, 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K, 
                     TOBECLONED pMFM039 pET28b-3xHA-sfGFP(A206K)-SGG-LPETGG-H6, 
                     TOBECLONED pMFM0xx pET28b-3xHA-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED pMFM0xx 
                     pMT2-SKP1dd-StrepTagII-truncFBXO31, 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6, 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy, TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep, 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005), 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy, 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003), 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V, 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp, 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002), 
                     pET28b_RH_ISG15_GyrA, pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V, pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG, pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S, A206K)-ASCC3_DxxD, 
                     pMFM032-pET28b-mCherry-SRT-His6, 
                     pMFM033-pET28b-mTagBFP2-SRT-His6, 
                     pMFM0xx_H6-3xHA-sfGFP-DxxD"
                     /note="/Transferred_Similarity=78.26%"
                     /note="/X-Transferred_From=A206K)-ASCC3 DxxD; A206K)-ASCC3 
                     RxxG;
                     A206K)-ASCC3_DxxD; R2S; TOBECLONED 
                     pET28b-His6-TEV-sfGFP_M1A_R2S_A206K-ASCC3_DxxD Copy; 
                     TOBECLONED pET28b-His6-TEV-sfGFP_R2S_A206K-ASCC3_DxxD; 
                     TOBECLONED pMFM pET28b-His6-TEV-sfGFP_A206K-GSX-K; 
                     TOBECLONED pMFM039 pET28b-3xHA-sfGFP(A206K)-SGG-LPETGG-H6; 
                     TOBECLONED pMFM0xx pET28b-3xHA-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6; 
                     TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM016_pET28b-Ser-sfGFP(A206K)-pep; 
                     TOBECLONED_pMFM020_pET28b_Ser-mNeonGreen-LPETGG-H6; 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep; old 
                     MFM16; pET28b-His6-TEV-sfGFP-ASCC3_DxxD (pMFM004); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005); 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK (pMFM005) Copy; 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG (pMFM003); 
                     pET28b-His6-TEV-sfGFP-LoopC-SGG-LPETGG_His6V; 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; 
                     pET28b-sfGFP-SGG-LPETGG_His6V (pMFM002); 
                     pET28b_RH_ISG15_GyrA; pMFM002 
                     pET28b-sfGFP-SGG-LPETGG_His6V; pMFM003 
                     pET28b-His6-TEV-sfGFP-ASCC3_RxxG; pMFM004 
                     pET28b-His6-TEV-sfGFP-ASCC3_DxxD; pMFM005 
                     pET28b-His6-TEV-sfGFP-ASCC3_KxxK; pMFM012 
                     pET28b-His6-TEV-sfGFP-pep_V5 temp; pMFM013 
                     pET28b-His6-TEV(G)-mCherry; pMFM014 
                     pET28b-His6-TEV-sfGFP-SRT-His6; pMFM016 mod; pMFM016 mod 
                     Copy; pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6; 
                     pMFM017 pET28b-His6-TEV-sfGFP_A206K-ASCC3; pMFM018 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_DxxD; pMFM019 
                     pET28b-His6-TEV-sfGFP_A206K-ASCC3_KxxK; pMFM020 
                     pET28b-His6-TEV(S)-mCherry; pMFM026 
                     pET28-His6-TEV-sfGFP_A206K-GSX-K-HA; pMFM027 
                     pET28b-His6-TEV-sfGFP(R2S; pMFM028 
                     pET28b-His6-TEV-sfGFP(R2S; pMFM029 
                     pET28b-His6-TEV-sfGFP(M1A; pMFM030 
                     pET28b-His6-TEV-sfGFP(M1A; to clone 
                     pET28b-His6-TEV-mCherry"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     CDS             4161..5948
                     /label=His6-Smt3-StrepII-FBXO31(del1-65)
                     /note="/created_by=matthiasmuhar"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4173..4190
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=X032-M16,
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 translation, 
                     pMFM032-pET28b-mCherry-SRT-His6 translation, 
                     pMFM033-pET28b-mTagBFP2-SRT-His6 translation, temp_gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED_pMFM020_pET28b_Ser-mN
                     eonGr
                     een-LPETGG-H6; 
                     TOBECLONED_pMFM0_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 
                     Copy; TOBECLONED_pMFM_pET28b-Ser-sfGFP(A206K)-pep; 
                     X032-M16; old MFM16; pMFM016 mod; pMFM016 mod Copy; 
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6; 
                     pMFM016_pET28b-Ser-sfGFP(A206K)-SGG-LPETGG-H6 translation; 
                     pMFM032-pET28b-mCherry-SRT-His6 translation; 
                     pMFM033-pET28b-mTagBFP2-SRT-His6 translation"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
                     /note="/codon_start=1"
                     /note="/modified_by=matthiasmuhar"
                     /note="/transl_table=1"
                     /note="color: #a6acb3"
     misc_feature    4197..4487
                     /gene="Smt3"
                     /label=Smt3
                     /label=nonstandard type:  misc
                     /note="Smt3 SUMO tag"
                     /note="/Transferred_From=TOBECLONED
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG, TOBECLONED 
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG - gBlock, pHYRSF53, 
                     temp_gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=TOBECLONED
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG; TOBECLONED 
                     pMFM034_pET28b-His6-Smt3-mCherry-RxxG - gBlock; pHYRSF53"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/codon_start=1"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    4494..4517
                     /label=StrepII Tag
                     /label=nonstandard type:  misc
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    4527..5948
                     /label=FBXO31(del_1-65)
                     /label=nonstandard type:  misc
                     /note="/created_by=matthiasmuhar"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #a6acb3"
     terminator      6074..6121
                     /label=T7 terminator
                     /note="transcription terminator for
                     bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     rep_origin      6158..6613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     misc_feature    6604
                     /label=T
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=T"
                     /note="color: #a6acb3"
     misc_feature    6613^6614
                     /label=C
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=C"
                     /note="color: #a6acb3"
     CDS             complement(6705..7520)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in
                     bacteria or G418 (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     3541 attttgttta actttaagaa ggagatatac atatggcgtc tatcaagttg cagtcatctg
     3601 acggagaaat ctttgaagtc gatgtcgaga tcgccaaaca gtctgtcacg atcaagacaa
     3661 tgttggaaga cttagggatg gaccctgttc ccttgcccaa tgtcaacgct gccattctga
     3721 aaaaggtaat tcaatggtgt acccaccata aagatgatcc tggtggcagc ggaacggatg
     3781 acattcccgt gtgggaccaa gagtttctga aggtagatca agggacgttg tttgagttaa
     3841 ttttagcagc caactactta gatattaagg ggttacttga tgtgacatgt aagacggtag
     3901 ccaacatgat taaaggtaag acgccagaag aaatccgtaa gacctttaat atcaagaatg
     3961 actttactga ggaggaggag gcccaagtgc gtaaggagaa ccagtggtgc gaagagaagt
     4021 aatgcttaag tcgaacagaa agtaatcgta ttgtacacgg ccgcataatc gaaattaata
     4081 cgactcacta taggggaatt gtgagcggat aacaattccc catcttagta tattagttaa
     4141 gtataagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcactagtg
     4201 actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc
     4261 acatcaattt aaaggtgtcc gatggatctt cagaaatctt ctttaagatc aaaaagacca
     4321 ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct
     4381 taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa gatttggaca
     4441 tggaggataa cgatattatt gagactcaca gagaacagat tggtggagcc agctggtcac
     4501 atcctcaatt tgagaagtcc ggtcgcagtc tgctggaact gcctcctgag ttacttgtag
     4561 aaatctttgc gtcgttgcct ggcactgatt tgccatccct tgctcaagta tgtaccaaat
     4621 ttcgtcgcat ccttcacact gataccatct ggcgtcgccg ttgtcgcgaa gagtatggtg
     4681 tttgtgaaaa cctgcgtaaa ttagaaatta ctggggtttc ttgccgtgac gtatatgcca
     4741 aactgcttca ccgttaccgc catattttgg gattatggca accagatatt ggtccatatg
     4801 gaggtctttt gaacgttgtt gtcgatggcc tttttattat cggttggatg tacctgccgc
     4861 ctcacgatcc acacgttgat gaccccatgc gctttaaacc cttgttccgc attcacttaa
     4921 tggagcgcaa agcagccacc gtagaatgta tgtacggcca taagggaccg catcatgggc
     4981 atattcaaat tgtcaagaaa gatgaattct ccactaaatg taaccaaaca gaccaccatc
     5041 gcatgagtgg tggtcgtcag gaggaattcc gtacatggtt gcgcgaagaa tggggacgca
     5101 ccttggagga tatcttccac gaacatatgc aagaattaat cttgatgaag tttatctaca
     5161 cttcgcagta tgataattgt ttaacttatc gtcgtattta cctgccaccc tcgcgtccag
     5221 atgaccttat taaaccgggt ctgttcaagg gtacatatgg atcacacggt ttggaaatcg
     5281 tgatgttgtc ttttcatggc cgtcgtgcgc gtggaacgaa aatcacaggt gatccgaata
     5341 tcccagccgg gcaacaaact gtcgaaattg atctgcgtca tcgcattcag ctgcccgacc
     5401 tggagaacca acgtaatttc aacgaattgt cacgcatcgt tctggaggtc cgtgagcgtg
     5461 tccgccagga gcagcaagag ggtggacatg aggccggtga agggcgtggt cgtcagggcc
     5521 cgcgcgagtc tcaaccatct ccagctcaac cacgcgcgga agcccccagt aaagggcctg
     5581 atggcacgcc aggggaagat ggtggtgaac cgggggacgc tgttgcggct gccgaacagc
     5641 ccgctcagtg tgggcagggc cagccatttg tgctgcccgt aggggtgagc tcccgtaatg
     5701 aagattatcc acgtacttgt cgcatgtgct tctatggcac gggtctgatc gcagggcacg
     5761 ggtttacatc accggaacgc acacccggtg tgttcatctt attcgacgag gaccgttttg
     5821 gtttcgtttg gctggaactt aaatcattct cgttatatag ccgtgttcag gctactttcc
     5881 gtaatgcaga cgcccccagt ccccaagcat ttgacgagat gttaaaaaat atccagagcc
     5941 tgacttcata ataaggatcc gcgatcgcgg cgcgccacct ggtggccggc cggtaccacg
     6001 cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     6061 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     6121 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     6181 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6241 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6301 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6361 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6421 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6481 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6541 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6601 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6661 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
     6721 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     6781 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     6841 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     6901 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     6961 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     7021 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     7081 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     7141 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     7201 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     7261 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     7321 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     7381 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     7441 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     7501 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     7561 attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7621 agaaaagatc aaaggatctt c
//
