LOCUS       Exported               16315 bp ds-DNA     circular SYN 26-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    NEBuilder.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16315)
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported 15-NOV-2021 from NEBuilder https://nebuilder.neb.com
REFERENCE   2  (bases 1 to 16315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase = 
            overlap) LSL(SPH)_fwd: 
            ttggcaaagaattgtgtacagctagcTTTGATACCGCGGGCCCTAAATAAC   LSL(SPH)_fwd 
            3'Tm: 69.2  3'Ta: 70.2
            LSL(SPH)_rev: acttcttgtccatggtggcactagTTGGGCTGCAGGTCGAGG 
            LSL(SPH)_rev 3'Tm: 70.9  3'Ta: 70.2.
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      2..11
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /note="/Transferred_From=Exported, Platt LSL, TOBECLONED
                     pC13N_delta_loxP, mod TOBECLONED pC13N_delta_loxP Copy, 
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy, test gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Platt LSL; modv2 step1
                     pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy; test gBlock"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     CDS             8..4111
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /note="/Transferred_From=Exported, TOBECLONED 
                     pC13N_delta_loxP,
                     mod TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MDKKYSIGLAIGTNSVGWAVITDEYKV
                     PSKKF 
                     KVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEM 
                     AKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDK 
                     ADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGV 
                     DAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKL 
                     QLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMI 
                     KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPIL 
                     EKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNRE 
                     KIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT 
                     NFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFK 
                     TNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEEN 
                     EDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGI 
                     RDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLA 
                     GSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEE 
                     GIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQ 
                     SFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK 
                     AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKS 
                     KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDV 
                     RKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDK 
                     GRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGF 
                     DSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKK 
                     DLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE 
                     DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAEN 
                     IIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG 
                     GD"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MDKKYSIGLAIGTNSVGWAVITDEY
                     KVPSK 
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN 
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST 
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS 
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA 
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS 
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP 
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN 
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER 
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL 
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE 
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN 
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN 
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI 
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIV 
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL 
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL 
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY 
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW 
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG 
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV 
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS 
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA 
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ 
                     LGGD"
                     /note="color: #993366"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4115..4141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="/Transferred_From=Exported, TOBECLONED 
                     pC13N_delta_loxP,
                     mod TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=YPYDVPDYA"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=YPYDVPDYA"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
     CDS             4160..4180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Transferred_From=Exported, TOBECLONED 
                     pC13N_delta_loxP,
                     mod TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=PKKKRKV"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=PKKKRKV"
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             4187..4207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Transferred_From=Exported, TOBECLONED 
                     pC13N_delta_loxP,
                     mod TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=PKKKRKV"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=PKKKRKV"
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             4247..4942
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=SELIKENMHMKLYMEGTVDNHHFKCTS
                     EGEGK 
                     PYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWE 
                     RVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYP 
                     ADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANN 
                     ETYVEQHEVAVARYCDLPSKLGHKLN"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=SELIKENMHMKLYMEGTVDNHHFKC
                     TSEGE 
                     GKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFT 
                     WERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETL 
                     YPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEA 
                     NNETYVEQHEVAVARYCDLPSKLGHKLN"
                     /note="color: #993366"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             4988..5173
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=RTLVTFKDVFVDFTREEWKLLDTAQQI
                     VYRNV MLENYKNLVSLGYQLTKPDVILRLEKGEEP"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=RTLVTFKDVFVDFTREEWKLLDTAQ
                     QIVYR NVMLENYKNLVSLGYQLTKPDVILRLEKGEEP"
                     /note="color: #993366"
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     polyA_signal    5331..5386
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     polyA_signal    complement(5331..5386)
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="/Transferred_From=Nora et al 2020"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    complement(5719..5752)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with 
                     eachother, but incompatible with wild-type loxP sites."
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    5794..6991
                     /label=cHS4 insulator
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    7004..7801
                     /label=CLYBL HR
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=CLYBL NC_000013, CLYBL NC_000013 -
                     CLYBL HR 2, TOBECLONED pC13N_delta_loxP, mod TOBECLONED 
                     pC13N_delta_loxP Copy, modv2 step1 pC13N-dCas9-BFP-KRAB 
                     (Addgene #127968), pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=CLYBL NC_000013; CLYBL NC_000013
                     -
                     CLYBL HR 2; modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene 
                     #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3"
     CDS             8316..9125
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MSHIQRETSRPRLNSNMDADLYGYKWA
                     RDNVG 
                     QSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPD 
                     DAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLA 
                     QAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDE 
                     GKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLM 
                     LDEFF"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MSHIQRETSRPRLNSNMDADLYGYK
                     WARDN 
                     VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRT 
                     PDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFR 
                     LAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIF 
                     DEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFH 
                     LMLDEFF"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      9303..9891
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/Transferred_From=Exported, Nora et al 2020, Platt 
                     LSL,
                     TOBECLONED pC13N_delta_loxP, mod TOBECLONED 
                     pC13N_delta_loxP Copy, modv2 step1 pC13N-dCas9-BFP-KRAB 
                     (Addgene #127968), pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Nora et al 2020; Platt
                     LSL;
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffff00"
     protein_bind    10179..10200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="/Transferred_From=Exported, Nora et al 2020, 
                     TOBECLONED
                     pC13N_delta_loxP, mod TOBECLONED pC13N_delta_loxP Copy, 
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Nora et al 2020; modv2
                     step1 pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     promoter        10215..10245
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="/Transferred_From=Exported, Nora et al 2020, Platt 
                     LSL,
                     TOBECLONED pC13N_delta_loxP, mod TOBECLONED 
                     pC13N_delta_loxP Copy, modv2 step1 pC13N-dCas9-BFP-KRAB 
                     (Addgene #127968), pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Nora et al 2020; Platt
                     LSL;
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     protein_bind    10253..10269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/Transferred_From=Exported, Nora et al 2020, 
                     TOBECLONED
                     pC13N_delta_loxP, mod TOBECLONED pC13N_delta_loxP Copy, 
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Nora et al 2020; modv2
                     step1 pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     misc_feature    10313..11145
                     /label=CLYBL HR 1
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=CLYBL NC_000013, CLYBL NC_000013 -
                     CLYBL HR 1, TOBECLONED pC13N_delta_loxP, mod TOBECLONED 
                     pC13N_delta_loxP Copy, modv2 step1 pC13N-dCas9-BFP-KRAB 
                     (Addgene #127968), pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=CLYBL NC_000013; CLYBL NC_000013
                     -
                     CLYBL HR 1; modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene 
                     #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    11154..11179
                     /label=SA
                     /note="splice acceptor site"
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     CDS             11203..11256
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding 
                     separatepolypeptides."
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=EGRGSLLTCGDVEENPGP"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=EGRGSLLTCGDVEENPGP"
                     /note="color: #993366"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11260..12051
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=IEQDGLHAGSPAAWVERLFGYDWAQQT
                     IGCSD 
                     AAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDW 
                     LLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRME 
                     AGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFI 
                     DCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=IEQDGLHAGSPAAWVERLFGYDWAQ
                     QTIGC 
                     SDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGR 
                     DWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTR 
                     MEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSG 
                     FIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEF 
                     F"
                     /note="color: #ccffcc"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     11489..11508
                     /label=11,489 F
                     /note="color: black; sequence: CAGCTGTGCTCGACGTTGTC"
     polyA_signal    12086..12310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="/Transferred_From=Exported, Nora et al 2020, Platt 
                     LSL,
                     TOBECLONED pC13N_delta_loxP, mod TOBECLONED 
                     pC13N_delta_loxP Copy, modv2 step1 pC13N-dCas9-BFP-KRAB 
                     (Addgene #127968), pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy, syn LSL - source synthetic DNA construct, test 
                     gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Nora et al 2020; Platt
                     LSL;
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy; syn LSL - 
                     source synthetic DNA construct; test gBlock"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     primer_bind     complement(12291..12330)
                     /label=Primer
                     /note="color: black; sequence: 
                     GGGGCTGTCCCCGTGAGCTCATAATTCGAACCATAGAGCCCACCGCA
                     TCC"
     misc_feature    complement(12291..12310)
                     /label=oMFM297-stub
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    12311..12313
                     /label=CAATT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CAATT"
                     /note="color: #a6acb3"
     misc_feature    12315..12316
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
     misc_feature    12330..13527
                     /label=cHS4 insulator
                     /label=nonstandard type:  misc
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #a6acb3"
     protein_bind    13546..13579
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with 
                     eachother, but incompatible with loxP or loxN sites (Lee 
                     andSaito, 1988)."
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy, modv2 step1 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=modv2 step1 pC13N-dCas9-BFP-KRAB
                     (Addgene #127968); pC13N-dCas9-BFP-KRAB (Addgene #127968) 
                     Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     enhancer        13650..13953
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="/Transferred_From=Exported, Platt LSL, TOBECLONED
                     pC13N_delta_loxP, mod TOBECLONED pC13N_delta_loxP Copy, 
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Platt LSL; modv2 step1
                     pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     primer_bind     complement(13785..13804)
                     /label=13,804 R
                     /note="color: black; sequence: CCAAGTGGGCAGTTTACCGT"
     promoter        13955..14232
                     /label=chicken beta-actin promoter
                     /note="/Transferred_From=TOBECLONED pC13N_delta_loxP, mod
                     TOBECLONED pC13N_delta_loxP Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #ffffff; direction: RIGHT"
     intron          14233..15249
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="/Transferred_From=Exported, Platt LSL, TOBECLONED
                     pC13N_delta_loxP, mod TOBECLONED pC13N_delta_loxP Copy, 
                     modv2 step1 pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported; Platt LSL; modv2 step1
                     pC13N-dCas9-BFP-KRAB (Addgene #127968); 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="color: #------"
     protein_bind    15336..15369
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="/Transferred_From=Exported, Platt LSL, modv2 step1
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy, test gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     misc_feature    15370
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
                     /note="color: #a6acb3"
     misc_feature    15501^15502
                     /label=T
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=T"
                     /note="color: #a6acb3"
     misc_feature    15516
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
                     /note="color: #a6acb3"
     polyA_signal    15583..15717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="/Transferred_From=Exported, Platt LSL, syn LSL - 
                     source
                     synthetic DNA construct"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     polyA_signal    15837..15971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="/Transferred_From=Exported, Platt LSL, syn LSL - 
                     source
                     synthetic DNA construct"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     polyA_signal    16091..16225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="/Transferred_From=Exported, Platt LSL, syn LSL - 
                     source
                     synthetic DNA construct"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     protein_bind    16254..16287
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="/Transferred_From=Exported, Platt LSL, modv2 step1
                     pC13N-dCas9-BFP-KRAB (Addgene #127968), 
                     pC13N-dCas9-BFP-KRAB (Addgene #127968) Copy, test gBlock"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #31849b; direction: RIGHT"
ORIGIN
        1 tgccaccatg gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg
       61 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
      121 cgaccggcac agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggagaaac
      181 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg
      241 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt
      301 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat
      361 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
      421 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct
      481 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa
      541 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga
      601 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa
      661 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt
      721 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct
      781 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct
      841 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga
      901 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag
      961 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct
     1021 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg
     1081 ctacgccggc tacatcgatg gcggagccag ccaggaagag ttctacaagt tcatcaagcc
     1141 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct
     1201 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga
     1261 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga
     1321 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg
     1381 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt
     1441 cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt
     1501 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt
     1561 caccgtgtac aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc
     1621 cttcctgagc ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa
     1681 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt
     1741 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct
     1801 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga
     1861 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac
     1921 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg
     1981 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac
     2041 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca
     2101 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga
     2161 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct
     2221 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa
     2281 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg
     2341 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga
     2401 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa
     2461 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt
     2521 ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgataaca aagtgctgac
     2581 tcggagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa
     2641 gatgaagaac tactggcgcc agctgctgaa tgccaagctg attacccaga ggaagttcga
     2701 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa
     2761 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg
     2821 gatgaacact aagtacgacg agaacgacaa actgatccgg gaagtgaaag tgatcaccct
     2881 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat
     2941 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat
     3001 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt
     3061 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt
     3121 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg
     3181 gaagcggcct ctgatcgaga caaacggcga aacaggcgag atcgtgtggg ataagggccg
     3241 ggactttgcc accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac
     3301 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgacaa
     3361 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac
     3421 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa
     3481 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc
     3541 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct
     3601 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg
     3661 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct
     3721 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt
     3781 tgtggaacag cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa
     3841 gagagtgatc ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag
     3901 agacaagcct atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
     3961 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
     4021 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
     4081 acggatcgac ctgtctcagc tgggaggcga cgcctatccc tatgacgtgc ccgattatgc
     4141 cagcctgggc agcggctccc ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg
     4201 gaaagtggac ggcattggta gtgggagcaa cggcagcagc ggatccagcg agctgattaa
     4261 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     4321 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     4381 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     4441 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     4501 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     4561 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     4621 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     4681 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     4741 cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa
     4801 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     4861 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     4921 caaactgggg cacaagctta atggcggtgg cggagggatg gatgctaagt cactaactgc
     4981 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg
     5041 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa
     5101 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa
     5161 gggagaagag ccctagacgc gtgaattcac tcctcaggtg caggctgcct atcagaaggt
     5221 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     5281 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     5341 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     5401 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     5461 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     5521 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     5581 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     5641 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     5701 cttatggaga tcaccggtat aacttcgtat aatgtatact atacgaagtt atggtacctt
     5761 aattaagtta acatgcatag tactgagctc acggggacag ccccccccca aagcccccag
     5821 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     5881 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     5941 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     6001 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat
     6061 catagaacgg cctgggttgc aaaggagcac agtgctcatc cagatccaac cccctgctat
     6121 gtgcagggtc atcaaccagc agcccaggct gcccagagcc acatccagcc tggccttgaa
     6181 tgcctgcagg gatggggcat ccacagcctc cttgggcaac ctgttcagtg cgtcaccacc
     6241 ctctggggga aaaactgcct cctcatatcc aacccaaacc tcccctgtct cagtgtaaag
     6301 ccattccccc ttgtcctatc aagggggagt ttgctgtgac attgttggtc tggggtgaca
     6361 catgtttgcc aattcagtgc atcacggaga ggcagatctt ggggataagg aagtgcagga
     6421 cagcatggac gtgggacatg caggtgttga gggctctggg acactctcca agtcacagcg
     6481 ttcagaacag ccttaaggat aagaagatag gatagaagga caaagagcaa gttaaaaccc
     6541 agcatggaga ggagcacaaa aaggccacag acactgctgg tccctgtgtc tgagcctgca
     6601 tgtttgatgg tgtctggatg caagcagaag gggtggaaga gcttgcctgg agagatacag
     6661 ctgggtcagt aggactggga caggcagctg gagaattgcc atgtagatgt tcatacaatc
     6721 gtcaaatcat gaaggctgga aaagccctcc aagatcccca agaccaaccc caacccaccc
     6781 accgtgccca ctggccatgt ccctcagtgc cacatcccca cagttcttca tcacctccag
     6841 ggacggtgac ccccccacct ccgtgggcag ctgtgccact gcagcaccgc tctttggaga
     6901 aggtaaatct tgctaaatcc agcccgaccc tcccctggca caacgtaagg ccattatctc
     6961 tcatccaact ccaggacgga gtcagtgaga atattgttta aactcctcat ccttccaaca
     7021 taaatatatt ttgggattat atcaacattc aatgttactt aaagtgacct tgtaaatatt
     7081 ttcacaactg agccatgttt gatttgtata cttatgttta ctttactgtt tttcctgaag
     7141 ttaataattg ccttgaattt atttatttct ttaaaaatgt ttcattactc aggactgtag
     7201 tttacattac gattctttgt gttatacagt tgatgggttt cttttctttc ttaatttctt
     7261 taaaaaatag agatggggtc ttactatatt acccaggctg gtcttgaagt cctgggctca
     7321 agtgatcttc ctgtctcagc ctaccaagta gctgagacta taggtgcaaa aaagccacta
     7381 tacctggcta gtttacaggt tttaacaaat gcattatgcc acgtatccat tattacagga
     7441 tcacacaaga tattttcatt accctgaaag catccctgtg ttccaccaat tcatcctgcc
     7501 tccatgagcc gctggcaacc actgatctct atagttttgc cttttctaaa atgtcatata
     7561 attggaatca tacagtctgt agcattttca gactagcttt taaaatttgg caatatgcat
     7621 ttaaggttcc tccttaaatg tgaagggcat agccaatgtg gcttgatagc tcatttcttt
     7681 ttattggtga atatttcatt gtctggatgt tccacagttt gtttatccat tcacctattc
     7741 aatttgcttt ttttctgtgt atctatcact aattcaatac tggactctcc aacagagccg
     7801 tttaaacact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7861 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7921 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     7981 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     8101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gatgcagctc tggcccgtgt
     8221 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     8281 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     8341 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     8401 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     8461 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     8521 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     8581 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     8641 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     8701 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     8761 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     8821 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     8881 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     8941 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     9001 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     9061 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     9121 tctaatcaga attggttaat tggttgtaac attattcaga ttgggcttga tttaaaactt
     9181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    10081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    10141 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10201 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10261 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccgcggc cgcagcagga
    10321 gaatcatgcc aatgggccaa tatacattct gacccacagt ttcataataa aataaaatgg
    10381 ttgtggttgt aagccactat gtttcagagt ggtttgttac acagcaataa ataactaata
    10441 tagtaggcat accatcaagt ccaaagtagg tagagaagaa tgtaaatagc agagcaaaac
    10501 agcatgactg gtggctggga ggcttaaaac tgggacagga tcagagtcat gaaagaagtc
    10561 aaagaaatgg ttcagaagta aggctgagac tgacttacaa aagctgaaag tccctttaag
    10621 ttggtgtttg gtgcattggc aggggcaggt atggtgactt aaaagagcca tgctcaacaa
    10681 gatcaagcac aacacaatca cgggtcaccc cagcagacct tagcgagtct agccatttct
    10741 ttggtggtgg tcacagtcat gcttcagccc agtttccact tggacaaatg gtacatattt
    10801 tcaatgagat gaaaattaag atacaatcca tgtgctcaga gagtgatcac agctctgact
    10861 aaacactgtg ccccaaagtg ttgaggaatt gggaaaacct agctgagtta gtggtctctt
    10921 ttctgttaca ataaagctca taatgaaaat tagccttctt tgttcttccc caagtctttc
    10981 tttctagacg aaactacttt caactgtttt aacttcctta ctgttaactt ccatatttct
    11041 agatagtatg gtcagactgt tatttcttga cttttaaatg taagatatta tctactgact
    11101 tccttctatg taagatgagg attgagctct cttacccttc tcccaattta aatctgacct
    11161 cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt
    11221 ctaacatgcg gtgacgtgga ggagaatccc ggccctagga ttgaacaaga tggattgcac
    11281 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
    11341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
    11401 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
    11461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
    11521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    11581 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    11641 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
    11701 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    11761 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
    11821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    11881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    11941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    12001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttcta acacctggtg
    12061 catgacccgc aagcccggtg cctgactgtg ccttctagtt gccagccatc tgttgtttgc
    12121 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    12181 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    12241 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    12301 ggctctatgg ttcgaattat gagctcacgg ggacagcccc cccccaaagc ccccagggat
    12361 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
    12421 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
    12481 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
    12541 tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga cagaatcata
    12601 gaacggcctg ggttgcaaag gagcacagtg ctcatccaga tccaaccccc tgctatgtgc
    12661 agggtcatca accagcagcc caggctgccc agagccacat ccagcctggc cttgaatgcc
    12721 tgcagggatg gggcatccac agcctccttg ggcaacctgt tcagtgcgtc accaccctct
    12781 gggggaaaaa ctgcctcctc atatccaacc caaacctccc ctgtctcagt gtaaagccat
    12841 tcccccttgt cctatcaagg gggagtttgc tgtgacattg ttggtctggg gtgacacatg
    12901 tttgccaatt cagtgcatca cggagaggca gatcttgggg ataaggaagt gcaggacagc
    12961 atggacgtgg gacatgcagg tgttgagggc tctgggacac tctccaagtc acagcgttca
    13021 gaacagcctt aaggataaga agataggata gaaggacaaa gagcaagtta aaacccagca
    13081 tggagaggag cacaaaaagg ccacagacac tgctggtccc tgtgtctgag cctgcatgtt
    13141 tgatggtgtc tggatgcaag cagaaggggt ggaagagctt gcctggagag atacagctgg
    13201 gtcagtagga ctgggacagg cagctggaga attgccatgt agatgttcat acaatcgtca
    13261 aatcatgaag gctggaaaag ccctccaaga tccccaagac caaccccaac ccacccaccg
    13321 tgcccactgg ccatgtccct cagtgccaca tccccacagt tcttcatcac ctccagggac
    13381 ggtgaccccc ccacctccgt gggcagctgt gccactgcag caccgctctt tggagaaggt
    13441 aaatcttgct aaatccagcc cgaccctccc ctggcacaac gtaaggccat tatctctcat
    13501 ccaactccag gacggagtca gtgagaatat tggcgcgccg tcgacataac ttcgtatagg
    13561 atactttata cgaagttatc aattcaggct agttattaat agtaatcaat tacggggtca
    13621 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
    13681 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
    13741 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
    13801 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
    13861 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
    13921 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
    13981 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
    14041 ttttgtgcag cggggggggg gggggggggg ggggggcgcg cgccgggggg ggcggggggg
    14101 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
    14161 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
    14221 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
    14281 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
    14341 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
    14401 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
    14461 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
    14521 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
    14581 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
    14641 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
    14701 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
    14761 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
    14821 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
    14881 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
    14941 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
    15001 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
    15061 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
    15121 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
    15181 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
    15241 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
    15301 ttgtgtacag ctagctttga taccgcgggc cctaaataac ttcgtatagc atacattata
    15361 cgaagttatc tcgtggcagc gagctaactt taaataattg gcattattta aagttatcgc
    15421 gatgaataaa tgaaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc
    15481 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    15541 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    15601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    15661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc
    15721 gactctagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
    15781 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    15841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    15901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    15961 atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt
    16021 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    16081 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    16141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    16201 caatgtatct tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt
    16261 cgtatagcat acattatacg aagttattag gtccctcgac ctgcagccca actag
//
