LOCUS       Exported               10976 bp ds-DNA     circular SYN 24-NOV-2025
DEFINITION  .
ACCESSION   urn.local...12-3uy0ryr
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pHR-hEF1a-FOXP3-IRES-GFP from 1 to 12512 ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     5'
                     LTR (truncated) (pJC2735 - pHR-EF1A-HA-FBXO31(?F-box, 
                     D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     5'
                     LTR (truncated) (pJC2732 - 
                     pHR-EF1A-HA-FBXO31(?F-box)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     5'
                     LTR (truncated) (pJC2734 - 
                     pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2729 - pLenti-X1-EF1A-HA-FBXO31(?F-box, 
                     D334N)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2723 - pLenti-X1-EF1A-HA-FBXO31-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:77/1; RU5
                     (pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 5' 
                     LTR
                     (truncated) (pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP)"
                     /note="/Transferred_From=pJC2731 - 
                     pHR-EF1A-HA-FBXO31-IRES-GFP"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=matthiasmuhar"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2744 - 
                     hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2729 - pLenti-X1-EF1A-HA-FBXO31(?F-box, 
                     D334N)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2723 - pLenti-X1-EF1A-HA-FBXO31-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2738 - 
                     pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     HIV-1 Psi (pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     HIV-1
                     Psi (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Transferred_From=pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
     misc_feature    790..1158
                     /label=dGag
                     /note="Derived using Geneious Prime 2022.0.1 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     dGag
                     (pJC2740 - pHR-EF1A-HA-FBXO31(D334N)-IRES-mTagBFP)"
                     /note="/Primary_Match=98.38%; Adjusted-Interval-0:0/0; dGag
                     (dGag - RRL)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     dGag (pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP)"
                     /note="/Transferred_From=dGag - RRL"
                     /note="/Transferred_From=pJC2739 -
                     pHR-EF1A-HA-FBXO31-IRES-mTagBFP"
                     /note="/Transferred_Similarity=98.38%"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #f58a5e"
     misc_feature    1303..1535
                     /label=RRE
                     /note="/translation=MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVI
                     DAAPI 
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP 
                     PGLKISGRPLAVRWSYFCFALPLSCLKLPWCLLSLSFDAHNRGLLLYYIMI*"
                     /note="color: #ffc0cb"
     misc_feature    2051..2200
                     /label=cPPT/CTS
                     /note="color: #5dfffa"
     promoter        2228..3398
                     /label=hEF1alpha promoter
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #2c9c57"
     misc_feature    complement(3449..3469)
                     /label=attB2
                     /label=nonstandard type:  attB2
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3506..4078
                     /label=IRES
                     /note="/translation=MNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSL
                     WSEYH 
                     DDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFA 
                     PVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
                     /note="color: #ffc0cb"
     CDS             4082..4798
                     /label=EGFP
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     EGFP
                     (pJC2735 - pHR-EF1A-HA-FBXO31(?F-box, D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     EGFP
                     (pJC2732 - pHR-EF1A-HA-FBXO31(?F-box)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     EGFP
                     (pJC2734 - pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     EGFP
                     (pJC2733 - pHR-EF1A-HA-FBXO31(?F-box, T343V)-IRES-GFP)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     EGFP (pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP)"
                     /note="/Transferred_From=pJC2731 - 
                     pHR-EF1A-HA-FBXO31-IRES-GFP"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #b1ff67; direction: RIGHT"
     misc_feature    4829..5429
                     /label=WPRE
                     /label=nonstandard type:  motif
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE (pJC2297 - pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1)"
                     /note="/Transferred_From=pJC2297 -
                     pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=matthiasmuhar"
                     /note="color: #a6acb3"
     misc_feature    5520..5541
                     /label=U3PPT
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDLLFACTGS 
                     LWLDQI*"
                     /note="color: #ffc0cb"
     LTR             5537..5770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2744 - 
                     hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2735 - pHR-EF1A-HA-FBXO31(?F-box, 
                     D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2732 - 
                     pHR-EF1A-HA-FBXO31(?F-box)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     3'
                     LTR (Delta-U3) (pJC2734 - 
                     pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:53/1; RU5
                     (pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 3' 
                     LTR
                     (Delta-U3) (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Transferred_From=pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #ffe4c4"
     gene            6051..6314
                     /gene="tet (300 - 563)"
                     /label=tet (300 - 563)
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDLLFACTGS 
                     LWLDQI*"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        6601..6705
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2727 - 
                     pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2729 - pLenti-X1-EF1A-HA-FBXO31(?F-box, 
                     D334N)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2723 - pLenti-X1-EF1A-HA-FBXO31-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2738 - 
                     pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2726 - 
                     pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     promoter (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Transferred_From=pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     CDS             6706..7566
                     /label=AMP
                     /label=AMP; AmpR; ORF frame 3; ampR
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/%_Identity=100"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AMP;
                     AmpR; ORF frame 3; ampR (pJC2723 - 
                     pLenti-X1-EF1A-HA-FBXO31-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AMP;
                     AmpR; ORF frame 3; ampR (pJC2728 - 
                     pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2738 - pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2726 - pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Mismatches=0"
                     /note="/Motif=ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
                     GCATT 
                     TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT 
                     CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTG 
                     AGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATG 
                     TGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC 
                     TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATG 
                     GCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC 
                     CAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC 
                     ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC 
                     CAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACT 
                     ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG 
                     GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTG 
                     CTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCC 
                     AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG 
                     GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AMP;
                     AmpR; ORF frame 3; ampR (pJC2729 - 
                     pLenti-X1-EF1A-HA-FBXO31(?F-box, D334N)-PGK-Neo)"
                     /note="/Transferred_From=pJC2729 -
                     pLenti-X1-EF1A-HA-FBXO31(?F-box, D334N)-PGK-Neo"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=ampR "
                     /note="/X-Transferred_Similarity=99.77%"
                     /note="/vntifkey=4"
                     /note="color: #993366; direction: RIGHT"
     rep_origin      7721..8340
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="color: #ffc0cb"
     promoter        8584..8852
                     /label=SV40_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="color: #ffc0cb"
     rep_origin      8751..8886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     ori (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     ori (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     ori (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     ori (pJC2744 - 
                     hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     origin (pJC2738 - 
                     pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     origin (pJC2737 - pHR-EF1A-DD-3xFLAG-FBXO31-IRES-GFP)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40 ori
                     (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Transferred_From=pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #ffff00"
     CDS             10178..10651
                     /codon_start=1
                     /label=ORF frame 1
                     /note="color: #ffc0cb"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSN
                     RTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYA
                     QKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET"
     polyA_signal    10674..10795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2727 - 
                     pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2729 - pLenti-X1-EF1A-HA-FBXO31(?F-box, 
                     D334N)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2743 - 
                     hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2723 - 
                     pLenti-X1-EF1A-HA-FBXO31-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2726 - 
                     pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2742 - 
                     hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2745 - 
                     hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2744 - 
                     hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (pJC2728 - 
                     pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro)"
                     /note="/Transferred_From=pJC2741 - 
                     hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2221 gaattctgtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2281 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2341 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2401 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2461 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2521 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2581 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2641 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2701 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2761 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2821 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     2881 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     2941 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3001 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3061 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3121 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3181 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3241 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3301 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3361 gtggttcaaa gtttttttct tccatttcag gtgtcgtgga attctcacgc gtggatccgc
     3421 ggccgccccg ggttaattaa tctagaccca gctttcttgt acaaagtggt gacgacggta
     3481 tcgataagct tgatatcgaa ttccgccccc ccccctaacg ttactggccg aagccgcttg
     3541 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     3601 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     3661 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     3721 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     3781 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     3841 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     3901 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     3961 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4021 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     4081 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4141 cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta
     4201 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4261 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4321 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4381 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4441 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4501 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4561 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4621 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4681 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4741 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4801 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     4861 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4921 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4981 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5041 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5101 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5161 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5221 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5281 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5341 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5401 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     5461 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5521 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5581 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5761 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     5821 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     5881 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     5941 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     6001 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     6061 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     6121 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     6181 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     6241 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     6301 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6361 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6421 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6481 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     6541 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6841 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6901 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6961 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7021 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7081 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7141 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7201 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7261 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7321 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7381 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7441 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7501 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7561 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7861 agagcgcaga taccaaatac tgtctttcta gtgtagccgt agttaggcca ccacttcaag
     7921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8401 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8461 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8521 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     8581 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8641 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8701 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8881 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     8941 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     9001 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     9061 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     9121 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     9181 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     9241 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     9301 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     9361 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     9421 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     9481 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     9541 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     9601 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     9661 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     9721 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     9781 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     9841 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     9901 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     9961 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
    10021 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
    10081 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
    10141 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
    10201 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
    10261 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
    10321 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
    10381 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
    10441 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
    10501 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
    10561 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    10621 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    10681 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10741 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10801 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10861 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
    10921 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
