LOCUS       pIND21-puro_SOX1       11880 bp DNA     circular SYN 17-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Nov 26, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11880
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..634
                     /gene="miscellaneous"
                     /label=LTR
                     /note="LTR"
     misc_feature    1
                     /gene="3' linker"
                     /label=""
                     /note=""
     misc_feature    1
                     /gene="5' linker"
                     /label=""
                     /note=""
     misc_feature    454..634
                     /gene="miscellaneous"
                     /label=HIV-1-5 LTR (LTR-SIN)
                     /note="HIV-1-5 LTR (LTR-SIN)"
     misc_feature    686..823
                     /gene="miscellaneous"
                     /label=Psi
                     /note="Psi"
     misc_feature    1314..1518
                     /gene="miscellaneous"
                     /label=RRE
                     /note="RRE"
     misc_feature    2062..2077
                     /gene="miscellaneous"
                     /label=cPPT
                     /note="cPPT"
     misc_feature    2217..2650
                     /gene="promoter"
                     /label=TRE2
                     /note="TRE2"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2701..2708
                     /gene="recombination site"
                     /label=attR1
                     /note="attR1"
     misc_feature    2709..2727
                     /gene="recombination site"
                     /label=misc_recombination
                     /label=nonstandard type:  misc_recombination
                     /note="attL1"
                     /note="/SECDrawas=Region"
     misc_feature    2709..2718
                     /gene="5' cloning site"
                     /label=attL1 OpenFreezer Core Recombination Site
                     /note=" attL1 OpenFreezer Core Recombination Site"
                     /note="/SECDrawas=Region"
     CDS             2731..4129
                     /codon_start=1
                     /gene="Sox10_Olig2_Nkx6.2"
                     /label=Sox10
                     /note="accession: "
                     /note="/SECDrawas=Gene"
                     /note="/organism="
                     /translation="MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRAS
                     PGPGELGKVKKEQQDGEADDDKFPVCIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHV
                     KRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESDKRPFIEEAERLRMQ
                     HKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGTAAIQAHYKSAHLDHRHPGEGSP
                     MSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSMGEGGKPHIDFGNVDIGEI
                     SHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGV
                     ALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQ
                     FDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQP
                     VYTTLSRP"
     misc_feature    2731..4128
                     /gene="cdna name"
                     /label=hSOX10
                     /note="hSOX10"
                     /note="/SECDrawas=Region"
     primer_bind     3131..3152
                     /label=Sox10 401
     primer_bind     3531..3550
                     /label=Sox10 801
     primer_bind     3931..3950
                     /label=Sox 10 1201
     protein_bind    complement(4130..4154)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4140..4154
                     /gene="recombination site"
                     /label=attR2
                     /note="attR2"
     CDS             4201..4289
                     /gene="3xFLAG_BstBI"
                     /label=3xFLAG_BstBI
                     /note="accession:;"""
     misc_feature    4201..4206
                     /gene="5' cloning site"
                     /label=BstBI
                     /note="BstBI"
     CDS             4205..4276
                     /gene="tag"
                     /label=tag
                     /note="Triple Flag"
     misc_feature    4284..4289
                     /gene="3' cloning site"
                     /label=BstBI
                     /note="BstBI"
     misc_feature    4531..5721
                     /gene="promoter"
                     /label=EF-1alpha
                     /note="EF-1alpha"
     misc_feature    5824..6401
                     /gene="coding sequence"
                     /label=rtTA
                     /note="rtTA"
     misc_feature    6606..7195
                     /gene="promoter"
                     /label=IRES
                     /note="IRES"
     CDS             7228..7827
                     /gene="selectable marker"
                     /label=selectable marker
                     /note="Puromycin Resistance"
     misc_feature    7830..8090
                     /gene="miscellaneous"
                     /label=NeoR/KanR (540 - 800) - not translated
                     /note="NeoR/KanR (540 - 800) - not translated"
     misc_feature    8272..8847
                     /gene="miscellaneous"
                     /label=WPRE
                     /note="WPRE"
     misc_feature    8916..8931
                     /gene="miscellaneous"
                     /label=cPPT
                     /note="cPPT"
     misc_feature    8986..9166
                     /gene="miscellaneous"
                     /label=HIV-1-5 LTR (LTR-SIN)
                     /note="HIV-1-5 LTR (LTR-SIN)"
     misc_feature    9406..9434
                     /gene="promoter"
                     /label=Ampicillin
                     /note="Ampicillin"
     CDS             9494..10333
                     /gene="selectable marker"
                     /label=selectable marker
                     /note="Ampicillin Resistance"
     rep_origin      10491..11110
                     /gene="origin of replication"
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccacc atggcggagg agcaggacct atcggaggtg
     2761 gagctgagcc ccgtgggctc ggaggagccc cgctgcctgt ccccggggag cgcgccctcg
     2821 ctagggcccg acggcggcgg cggcggatcg ggcctgcgag ccagcccggg gccaggcgag
     2881 ctgggcaagg tcaagaagga gcagcaggac ggcgaggcgg acgatgacaa gttccccgtg
     2941 tgcatccgcg aggccgtcag ccaggtgctc agcggctacg actggacgct ggtgcccatg
     3001 cccgtgcgcg tcaacggcgc cagcaaaagc aagccgcacg tcaagcggcc catgaacgcc
     3061 ttcatggtgt gggctcaggc agcgcgcagg aagctcgcgg accagtaccc gcacctgcac
     3121 aacgctgagc tcagcaagac gctgggcaag ctctggaggc tgctgaacga aagtgacaag
     3181 cgccccttca tcgaggaggc tgagcggctc cgtatgcagc acaagaaaga ccacccggac
     3241 tacaagtacc agcccaggcg gcggaagaac gggaaggccg cccagggcga ggcggagtgc
     3301 cccggtgggg aggccgagca aggtgggacc gccgccatcc aggcccacta caagagcgcc
     3361 cacttggacc accggcaccc aggagagggc tcccccatgt cagatgggaa ccccgagcac
     3421 ccctcaggcc agagccatgg cccacccacc cctccaacca ccccgaagac agagctgcag
     3481 tcgggcaagg cagacccgaa gcgggacggg cgctccatgg gggagggcgg gaagcctcac
     3541 atcgacttcg gcaacgtgga cattggtgag atcagccacg aggtaatgtc caacatggag
     3601 acctttgatg tggctgagtt ggaccagtac ctgccgccca atgggcaccc aggccatgtg
     3661 agcagctact cagcagccgg ctatgggctg ggcagtgccc tggccgtggc cagtggacac
     3721 tccgcctgga tctccaagcc accaggcgtg gctctgccca cggtctcacc acctggtgtg
     3781 gatgccaaag cccaggtgaa gacagagacc gcggggcccc aggggccccc acactacacc
     3841 gaccagccat ccacctcaca gatcgcctac acctccctca gcctgcccca ctatggctca
     3901 gccttcccct ccatctcccg cccccagttt gactactctg accatcagcc ctcaggaccc
     3961 tattatggcc actcgggcca ggcctctggc ctctactcgg ccttctccta tatggggccc
     4021 tcgcagcggc ccctctacac ggccatctct gaccccagcc cctcagggcc ccagtcccac
     4081 agccccacac actgggagca gccagtatat acgacactgt cccggcccga cccagctttc
     4141 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     4201 ttcgaagact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     4261 gacgatgaca agtaggtaag tgattcgaat acccatacga cgtcccagac tacgcttagt
     4321 aatgattaat taaactagaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
     4381 ggagtaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     4441 ccctcctcac ggcgagccgc gtaagatctg gcctccgcgc cgggttttgg cgcctcccgc
     4501 gggcgccccc ctcctcacgg cgagcctagt cgtgaggctc cggtgcccgt cagtgggcag
     4561 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     4621 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     4681 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     4741 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     4801 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     4861 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     4921 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     4981 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     5041 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     5101 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     5161 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     5221 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     5281 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     5341 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     5401 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     5461 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     5521 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     5581 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     5641 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     5701 ttcaggtgtc gtgaagcggc cgccatgcta gtaaattgtc cgctaaattc tggccgtttt
     5761 tggctttttt gttagacgga tccataactt cgtataatgt atgctatacg aagttatgcc
     5821 accatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     5881 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     5941 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     6001 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     6061 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     6121 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     6181 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     6241 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     6301 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     6361 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     6421 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     6481 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     6541 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc gcactagagg
     6601 aattccgccc ctctcccccc cccccctctc cctccccccc ccctaacgtt actggccgaa
     6661 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     6721 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     6781 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     6841 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     6901 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     6961 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     7021 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     7081 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     7141 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
     7201 ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
     7261 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7321 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7381 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7441 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7501 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7561 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7621 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7681 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7741 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7801 tgcatgaccc gcaagcccgg tgcctgagca tgcccgacgg cgaggatctc gtcgtgaccc
     7861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgatgtacaa
     8101 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     8161 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     8221 gttgtttagt ccctcccaat tcgatatcaa gcttatcgcg atagatccta atcaacctct
     8281 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     8341 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8401 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8461 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8521 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8581 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8641 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     8701 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8761 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8821 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
     8881 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8941 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     9001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     9181 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
     9241 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
     9301 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     9361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9481 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9541 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9601 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9661 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     9721 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9781 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9841 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9901 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9961 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10021 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    10081 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    10141 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    10201 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    10261 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    10321 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10381 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    10441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10621 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    10981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    11221 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    11281 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
    11341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    11401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
    11461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11581 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
    11641 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11701 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    11761 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    11821 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
