LOCUS       12-N5KO-G1                13013 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     CDS             6965..11068
                     /note="pLannotate"
                     /label="Cas9"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    11819..12407
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      1..429
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3952..4331
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     CDS             886..1260
                     /note="pLannotate"
                     /label="BleoR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6322..6562
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    5394..5627
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             12479..12712
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     polyA_signal    12744..12968
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     LTR             4549..4729
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    4776..4901
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    1390..1511
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    6154..6271
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        complement(3582..3686)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2721..3581)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_RNA        6592..6667
                     /note="pLannotate"
                     /label="gRNA scaffold"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
     CDS             11206..11803
                     /note="pLannotate"
                     /label="PuroR (fragment)"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.8"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(1962..2550)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        820..867
                     /note="pLannotate"
                     /label="EM7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        443..772
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1608..1638)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1653..1674)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        6729..6940
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4332..4531
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4885..5248
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             6004..6104
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(1582..1604)
                     /note="pLannotate"
                     /label="lac operator (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="92.0"
                     /fragment="True"
                     /other="protein_bind"
     CDS             1270..1395
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3745..3797)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1687..1779)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    12458..12477
                     /note="pLannotate"
                     /label="cPPT/CTS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="16.9"
                     /fragment="True"
                     /other="misc_feature"
     promoter        12972..12993
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     CDS             6685..6703
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
      841 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
      901 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
      961 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     1021 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     1081 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     1141 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     1201 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     1261 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1381 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1501 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     3721 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     3781 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     3841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     3901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     3961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4321 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4441 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4501 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
     4561 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4621 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4681 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4741 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4801 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4861 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4921 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4981 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     5041 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     5101 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     5161 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     5221 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     5281 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5341 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5401 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5461 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5521 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5581 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5641 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5701 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5761 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5821 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5881 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5941 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     6001 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     6061 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     6121 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     6181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     6241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     6301 agtttggtta attaaggtac cgagggccta tttcccatga ttccttcata tttgcatata
     6361 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
     6421 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     6481 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     6541 ttatatatct tgtggaaagg acgaaacacc gaatcagtga aacgtacaac cgttttagag
     6601 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     6661 tcggtgcttt tttgaattcg ctagctaggt cttgaaagga gtgggaattg gctccggtgc
     6721 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     6781 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     6841 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     6901 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gaccggttct agagcgctgc
     6961 caccatggac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
     7021 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     7081 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     7141 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     7201 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     7261 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     7321 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     7381 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     7441 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     7501 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     7561 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     7621 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     7681 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     7741 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     7801 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     7861 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     7921 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     7981 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     8041 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     8101 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     8161 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     8221 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     8281 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     8341 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     8401 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     8461 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     8521 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     8581 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     8641 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     8701 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     8761 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     8821 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     8881 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     8941 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     9001 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     9061 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     9121 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     9181 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     9241 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     9301 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     9361 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     9421 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     9481 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     9541 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     9601 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     9661 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     9721 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     9781 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     9841 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     9901 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     9961 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
    10021 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
    10081 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
    10141 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
    10201 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
    10261 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
    10321 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
    10381 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
    10441 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
    10501 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
    10561 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
    10621 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
    10681 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
    10741 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
    10801 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
    10861 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
    10921 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
    10981 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
    11041 gatcgacctg tctcagctgg gaggcgacaa gcgacctgcc gccacaaaga aggctggaca
    11101 ggctaagaag aagaaagatt acaaagacga tgacgataag ggatccggcg caacaaactt
    11161 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgaccg agtacaagcc
    11221 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    11281 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    11341 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    11401 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    11461 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    11521 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    11581 caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    11641 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    11701 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    11761 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa
    11821 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    11881 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    11941 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    12001 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    12061 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    12121 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    12181 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
    12241 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    12301 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    12361 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
    12421 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    12481 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
    12541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    12601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    12661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
    12721 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    12781 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    12841 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    12901 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    12961 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc ccc
//
