LOCUS       14-George-PacI-BFP-N5-Midi                 9267 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     misc_feature    6015..6603
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        6617..7116
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      8682..9110
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        4029..4332
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    3161..3394
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        2089..2315
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4333..4536
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             2316..2496
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             7927..8107
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     CDS             5335..5991
                     /note="pLannotate"
                     /label="NUDT5"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      8340..8475
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    2543..2668
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    8179..8300
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        9163..9267
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1..861
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             4636..5334
                     /note="pLannotate"
                     /label="TagBFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7137..7736
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1032..1620
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1944..1974
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1908..1929
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(8496..8514)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2043..2061
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1982..1998
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             8522..8677
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    3890..4006
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             2652..3015
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3771..3871
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             7804..7941
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="80.4"
                     /match_length="22.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1803..1895
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     2881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3781 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     3841 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     3901 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3961 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     4021 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4081 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4141 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4201 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4261 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4321 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4381 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4441 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4501 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     4561 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gagatatctt
     4621 cgaaggcgcg ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     4681 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     4741 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     4801 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     4861 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     4921 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     4981 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     5041 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     5101 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     5161 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     5221 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     5281 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taatatggag
     5341 agccaagaac caacggaatc ttctcagaat ggcaaacagt atatcatttc agaggagtta
     5401 atttcagaag gaaaatgggt caagcttgaa aaaacaacgt acatggatcc tactggtaaa
     5461 actagaactt gggaatcagt gaaacgtaca accaggaaag agcagactgc ggatggtgtc
     5521 gcggtcatcc ccgtgctgca gagaacactt cactatgagt gtatcgttct ggtgaaacag
     5581 ttccgaccac caatgggggg ctactgcata gagttccctg caggtctcat agatgatggt
     5641 gaaaccccag aagcagctgc tctccgggag cttgaagaag aaactggcta caaaggggac
     5701 attgccgaat gttctccagc ggtctgtatg gacccaggct tgtcaaactg tactatacac
     5761 atcgtgacag tcaccattaa cggagatgat gccgaaaacg caaggccgaa gccaaagcca
     5821 ggggatggag agtttgtgga agtcatttct ttacccaaga atgacctgct gcagagactt
     5881 gatgctctgg tagctgaaga acatctcaca gtggacgcca gggtctattc ctacgctcta
     5941 gcgctgaaac atgcaaatgc aaagccattt gaagtgccct tcttgaaatt ttaaattaaa
     6001 gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6421 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6601 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     6661 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     6721 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     6781 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     6841 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     6901 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     6961 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     7021 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     7081 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     7141 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7201 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7261 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7321 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7381 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7441 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7501 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7561 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7621 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7681 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     7741 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     7801 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7861 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     7921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8161 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8281 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8461 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8521 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8581 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8641 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8701 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8761 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8821 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8881 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8941 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9001 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9061 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9121 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9241 cttcaataat attgaaaaag gaagagt
//
