LOCUS       043-LV-HA-N5-SL2-C76Y-2                 8620 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     misc_feature    4182..4770
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        4784..5283
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      6849..7277
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        2843..3146
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    1975..2208
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        903..1129
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3147..3350
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             1130..1310
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             6094..6274
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     rep_origin      6507..6642
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    1357..1482
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    6346..6467
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        7330..7434
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7435..8295
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             5304..5903
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(8466..8620,1..434)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3510..4163
                     /note="pLannotate"
                     /label="NUDT5"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="99.5"
                     /fragment="False"
                     /other="CDS"
     promoter        758..788
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    722..743
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(6663..6681)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        857..875
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    796..812
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6689..6844
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2704..2820
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             1466..1829
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             2585..2685
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             5971..6108
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="80.4"
                     /match_length="22.8"
                     /fragment="True"
                     /other="CDS"
     CDS             617..709
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
       61 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      661 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      721 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      781 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      841 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
      901 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      961 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     1021 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     1081 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     1141 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1201 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1261 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1321 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1381 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1441 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1501 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1561 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1621 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1681 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1741 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1801 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1861 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1921 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1981 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     2041 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     2101 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     2161 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     2221 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     2281 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2341 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2401 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2461 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2521 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2581 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2641 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     2701 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2761 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     2821 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2881 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2941 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3001 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3061 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3121 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3181 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3241 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3301 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     3361 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     3421 tctagaggga tccaccggtc gccaccatgt acccctacga cgtgcccgac tacgccggct
     3481 atccgtatga tgtcccggac tatgcaggag agagccaaga accaacggaa tcttctcaga
     3541 atggcaaaca gtatatcatt tcagaggagt taatttcaga aggaaaatgg gtcaagcttg
     3601 aaaaaacaac gtacatggat cctactggta aaactagaac ttgggaatca gtgaaacgta
     3661 caaccaggaa agagcagact gcggatggtg tcgcggtcat ccccgtgctg cagagaacac
     3721 ttcactatga gtatatcgtt ctggtgaaac agttccgacc accaatgggg ggctactgca
     3781 tagagttccc tgcaggtctc atagatgatg gtgaaacccc agaagcagct gctctccggg
     3841 agcttgaaga agaaactggc tacaaagggg acattgccga atgttctcca gcggtctgta
     3901 tggacccagg cttgtcaaac tgtactatac acatcgtgac agtcaccatt aacggagatg
     3961 atgccgaaaa cgcaaggccg aagccaaagc caggggatgg agagtttgtg gaagtcattt
     4021 ctttacccaa gaatgacctg ctgcagagac ttgatgctct ggtagctgaa gaacatctca
     4081 cagtggacgc cagggtctat tcctacgctc tagcgctgaa acatgcaaat gcaaagccat
     4141 ttgaagtgcc cttcttgaaa ttttaaagcg gccgcgtcga caatcaacct ctggattaca
     4201 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4261 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4321 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4381 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4441 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4501 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4561 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4621 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4681 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4741 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     4801 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4861 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4921 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4981 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5041 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5101 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     5161 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5221 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5281 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5341 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5401 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5461 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5521 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5581 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5641 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5701 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5761 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5821 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5881 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     5941 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     6001 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     6061 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6121 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6181 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6241 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6301 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6481 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6661 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6721 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6781 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6841 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6901 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6961 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7021 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7081 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7141 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7201 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7261 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7321 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7381 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7441 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7501 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7561 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7621 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7681 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7741 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7801 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7861 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7921 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7981 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8041 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8101 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8161 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8221 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8281 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8581 ggcttcagca gagcgcagat accaaatact gttcttctag
//
