LOCUS       044-LV-HA-N5-Y74F                 8619 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     misc_feature    5953..6541
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        6555..7054
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      1..429
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        4615..4918
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    3747..3980
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        2675..2901
                     /note="pLannotate"
                     /label="RSV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4919..5122
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             2902..3082
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             7865..8045
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     rep_origin      8278..8413
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    3129..3254
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    8117..8238
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        482..586
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             587..1447
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7075..7674
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1618..2206
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             5281..5934
                     /note="pLannotate"
                     /label="NUDT5"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="99.5"
                     /fragment="False"
                     /other="CDS"
     promoter        2530..2560
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2494..2515
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(8434..8452)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2629..2647
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2568..2584
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             8460..8615
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4476..4592
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             3238..3601
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             4357..4457
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             7742..7879
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="80.4"
                     /match_length="22.8"
                     /fragment="True"
                     /other="CDS"
     CDS             2389..2481
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2401 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2461 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2521 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2581 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     2641 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     2701 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     2761 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     2821 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     2881 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3061 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     3121 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     3181 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     3241 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     3301 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     3361 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     3421 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     3481 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     3541 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     3601 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     3661 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     3721 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     3781 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     3841 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     3901 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     3961 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     4021 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     4081 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     4141 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     4201 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     4261 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     4321 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     4381 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     4441 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
     4501 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     4561 ttacaaaaac aaattacaaa attcaaaatt ttatcgataa gcttgggagt tccgcgttac
     4621 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4681 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4741 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4801 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4861 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4921 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4981 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     5041 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5101 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     5161 acgctgtttt gacctccata gaagacaccg actctagagg atccaccggt cgccaccatg
     5221 tacccctacg acgtgcccga ctacgccggc tatccgtatg atgtcccgga ctatgcagga
     5281 gagagccaag aaccaacgga atcttctcag aatggcaaac agtatatcat ttcagaggag
     5341 ttaatttcag aaggaaaatg ggtcaagctt gaaaaaacaa cgtacatgga tcctactggt
     5401 aaaactagaa cttgggaatc agtgaaacgt acaaccagga aagagcagac tgcggatggt
     5461 gtcgcggtca tccccgtgct gcagagaaca cttcacttcg agtgtatcgt tctggtgaaa
     5521 cagttccgac caccaatggg gggctactgc atagagttcc ctgcaggtct catagatgat
     5581 ggtgaaaccc cagaagcagc tgctctccgg gagcttgaag aagaaactgg ctacaaaggg
     5641 gacattgccg aatgttctcc agcggtctgt atggacccag gcttgtcaaa ctgtactata
     5701 cacatcgtga cagtcaccat taacggagat gatgccgaaa acgcaaggcc gaagccaaag
     5761 ccaggggatg gagagtttgt ggaagtcatt tctttaccca agaatgacct gctgcagaga
     5821 cttgatgctc tggtagctga agaacatctc acagtggacg ccagggtcta ttcctacgct
     5881 ctagcgctga aacatgcaaa tgcaaagcca tttgaagtgc ccttcttgaa attttaaagc
     5941 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6001 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6061 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6121 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6181 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6241 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6301 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6361 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6421 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6481 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6541 cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     6601 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     6661 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     6721 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     6781 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     6841 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     6901 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     6961 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     7021 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     7081 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7141 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7201 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7261 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7321 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7381 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7441 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7501 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7561 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7621 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     7681 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc
     7741 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7801 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     7861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8041 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8101 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8281 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8341 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8401 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8461 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8521 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8581 gcccttccca acagttgcgc agcctgaatg gcgaatggg
//
