LOCUS       212-lenti-GFP                 8927 bp    DNA     circular SYN 20-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     CDS             6589..7308
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    7333..7921
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      1..429
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3952..4331
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     CDS             886..1260
                     /note="pLannotate"
                     /label="BleoR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    5394..5627
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    8658..8882
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     LTR             4549..4729
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             8446..8626
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    4776..4901
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    1390..1511
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    6154..6271
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        complement(3582..3686)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2721..3581)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1962..2550)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        820..867
                     /note="pLannotate"
                     /label="EM7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        443..772
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1608..1638)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7858..8439
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="84.8"
                     /match_length="94.2"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(1653..1674)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        6364..6575
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4332..4531
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1584..1600)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4885..5248
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     CDS             6004..6104
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1270..1395
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3745..3797)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1687..1779)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    7309..7330
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
     promoter        8886..8907
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     CDS             6320..6338
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
      841 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
      901 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
      961 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     1021 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     1081 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     1141 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     1201 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     1261 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1381 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1501 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     3721 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     3781 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     3841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     3901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     3961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4321 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4441 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4501 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
     4561 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4621 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4681 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4741 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4801 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4861 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4921 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4981 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     5041 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     5101 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     5161 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     5221 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     5281 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5341 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5401 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5461 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5521 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5581 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5641 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5701 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5761 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5821 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5881 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5941 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     6001 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     6061 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     6121 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     6181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     6241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     6301 agtttggtta attagctagc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     6361 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     6421 gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     6481 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     6541 ttctttttcg caacgggttt gccgccagaa cacaggaccg gtgccaccat ggtgagcaag
     6601 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     6661 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     6721 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     6781 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     6841 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     6901 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     6961 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7021 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     7081 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     7141 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     7201 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     7261 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga attcgatatc
     7321 aagcttatcg gtaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7381 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7441 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7501 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7561 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7621 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7681 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7741 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7921 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     7981 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8041 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8101 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8161 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     8221 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8281 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8341 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     8401 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     8461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     8641 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8701 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8761 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8821 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8881 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatcccc
//
