LOCUS       pAAV                    5857 bp    DNA     circular     01-DEC-2025
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="AAV2"
     source          1..5857
                     /dnas_title="pAAV MCS Nter PG 80x GFP"
     misc_feature    150..155
                     /note="MLU1"
     misc_feature    156..807
                     /note="CMV"
     misc_feature    808..813
                     /note="SAC2"
     misc_feature    825..872
                     /note=""
     misc_feature    873..1251
                     /note="Intron"
     misc_feature    1252..1307
                     /note=""
     misc_feature    1323..1328
                     /note="ECOR1"
     misc_feature    1330..1335
                     /note="HIND3"
     misc_feature    1341..1345
                     /note="koza"
     misc_feature    1342..1344
                     /note="atg"
     misc_feature    1342..1797
                     /note="OPML op"
     misc_feature    1459..1758
                     /note="polyG"
     misc_feature    1798..1803
                     /note="BAMH1"
     misc_feature    1804..2517
                     /note="GFP op"
     misc_feature    2524..2529
                     /note="ECOR1"
     misc_feature    2557..2562
                     /note="XHO1"
     misc_feature    2563..2589
                     /note="HA"
     misc_feature    2596..2600
                     /note="BGL2"
     misc_feature    2614..3080
                     /note="hGH polyA+"
     misc_feature    3098..3103
                     /note="PML1"
     misc_feature    3120..3260
                     /note="AAV2LTR"
     misc_feature    3335..3795
                     /note="F1"
     misc_feature    4041..5037
                     /note="AMP R"
     misc_feature    5192..5811
                     /note="PBR322 ORI"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTCTAGT TATTAATAGT AATCAATTAC
      181 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
      241 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
      301 CATAGTAACG cCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
      361 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
      421 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
      481 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
      541 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
      601 CGTCAATGGG AGTTTGTTTT gGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
      661 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
      721 AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA
      781 TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGATTCGAA TCCCGGCCGG GAACGGTGCA
      841 TTGGAACGCG GATTCCCCGT GCCAAGAGTG ACgtaagtac cgcctataga gtctataggc
      901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gCTCCTGGGC
     1261 AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG AATTGGGatt cgaacatcga
     1321 ttGAATTCaA AGCTTtgctt cATGGAGCCT TGTGCCTGGG TGGGAGCCCC TGAGAGATCT
     1381 TGGCCTGCCG GAGGCCCTGA TGCCCTGAGG GGAAGAGATG GCGCTAAAGA GGCCGGAAGA
     1441 AGGAGACGCA AAAGAAGGGG CGGGGGGGGA GGGGGCGGGG GAGGCGGAGG CGGGGGAGGC
     1501 GGGGGCGGCG GAGGGGGAGG GGGGGGGGGA GGAGGCGGCG GGGGAGGAGG CGGAGGGGGG
     1561 GGCGGCGGCG GAGGGGGCGG GGGCGGAGGC GGCGGAGGGG GCGGAGGCGG AGGCGGGGGA
     1621 GGAGGAGGGG GCGGCGGAGG CGGAGGAGGC GGGGGAGGGG GCGGGGGAGG CGGCGGCGGG
     1681 GGCGGGGGGG GAGGGGGAGG AGGGGGAGGC GGAGGAGGAG GGGGCGGGGG CGGAGGGGGG
     1741 GGAGGCGGGG GCGGGGGAAG AGAGGAGCTG GAAGTGTTCC CTGGAAGACT GGAAGACGGA
     1801 TCCGTCAGCA AAGGCGAGGA GCTTTTCACC GGGGTTGTTC CCATCCTCGT CGAGCTCGAC
     1861 GGCGACGTAA ACGGCCACAA ATTCAGCGTT TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     1921 GGCAAGCTCA CCCTCAAGTT CATCTGCACC ACCGGCAAGC TCCCCGTCCC CTGGCCCACC
     1981 CTCGTCACCA CCCTCACCTA CGGCGTCCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     2041 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2101 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2161 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2221 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
     2281 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
     2341 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2401 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2461 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGtga
     2521 taaGAATTCt gcagatatcc agcacagtgg cggccgCTCG AGTACCCATA CGATGTTCCA
     2581 GATTACGCTA AGtaaAGATC tacgggtggc atcCCTGTGA CCCCTCCCCA GTGCCTCTCC
     2641 TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA
     2701 TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA
     2761 GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT
     2821 GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC
     2881 TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT
     2941 TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT
     3001 AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA
     3061 CTGCTCCCTT CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA
     3121 GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC
     3181 CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG
     3241 AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     3301 TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC
     3361 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC
     3421 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT
     3481 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA
     3541 AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC
     3601 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA
     3661 CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT
     3721 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG
     3781 TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG
     3841 CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC
     3901 GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA
     3961 TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC
     4021 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
     4081 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC
     4141 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
     4201 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG
     4261 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT
     4321 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC
     4381 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA
     4441 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
     4501 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT
     4561 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT
     4621 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT
     4681 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG
     4741 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
     4801 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT
     4861 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG
     4921 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG
     4981 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG
     5041 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA
     5101 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT
     5161 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT
     5221 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
     5281 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG
     5341 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA
     5401 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
     5461 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
     5521 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
     5581 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC
     5641 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA
     5701 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
     5761 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA
     5821 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGT
//

