LOCUS       pAAV                    5839 bp    DNA     circular     01-DEC-2025
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="AAV2"
     source          1..5839
                     /dnas_title="pAAV GIPC1 polyGly GFP"
     misc_feature    150..155
                     /note="MLU1"
     misc_feature    156..807
                     /note="CMV"
     misc_feature    808..813
                     /note="SAC2"
     misc_feature    825..872
                     /note=""
     misc_feature    839..843
                     /note="Kozac"
     misc_feature    840..842
                     /note="att"
     misc_feature    846..848
                     /note="acg"
     misc_feature    852..854
                     /note="att"
     misc_feature    864..866
                     /note="aag"
     misc_feature    873..1251
                     /note="Intron"
     misc_feature    1252..1307
                     /note=""
     misc_feature    1254..1258
                     /note="Kozac"
     misc_feature    1255..1257
                     /note="ctg"
     misc_feature    1264..1266
                     /note="gtg"
     misc_feature    1267..1269
                     /note="ctg"
     misc_feature    1276..1278
                     /note="gtg"
     misc_feature    1279..1281
                     /note="ctg"
     misc_feature    1303..1305
                     /note="ttg"
     misc_feature    1321..1323
                     /note="ttg"
     misc_feature    1323..1328
                     /note="ECOR1"
     misc_feature    1330..1335
                     /note="HIND3"
     misc_feature    1330..1332
                     /note="aag"
     misc_feature    1336..1341
                     /note="Kozac"
     misc_feature    1342..1700
                     /note="Exon 1 op GIPC1"
     misc_feature    1342..1344
                     /note="ctg"
     misc_feature    1357..1363
                     /note="Kozac"
     misc_feature    1360..1779
                     /note="PolyGlycine Frame"
     misc_feature    1360..1362
                     /note="ctg"
     misc_feature    1411..1695
                     /note="polygly op"
     misc_feature    1701..1779
                     /note="Exon 4 op"
     misc_feature    1780..1785
                     /note="BAMH1"
     misc_feature    1786..2499
                     /note="GFP op"
     misc_feature    2506..2511
                     /note="ECOR1"
     misc_feature    2539..2544
                     /note="XHO1"
     misc_feature    2545..2571
                     /note="HA"
     misc_feature    2578..2582
                     /note="BGL2"
     misc_feature    2596..3062
                     /note="hGH polyA+"
     misc_feature    3080..3085
                     /note="PML1"
     misc_feature    3102..3242
                     /note="AAV2LTR"
     misc_feature    3317..3777
                     /note="F1"
     misc_feature    4023..5019
                     /note="AMP R"
     misc_feature    5174..5793
                     /note="PBR322 ORI"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTCTAGT TATTAATAGT AATCAATTAC
      181 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
      241 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
      301 CATAGTAACG cCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
      361 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
      421 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
      481 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
      541 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
      601 CGTCAATGGG AGTTTGTTTT gGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
      661 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
      721 AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA
      781 TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGATTCGAA TCCCGGCCGG GAACGGTGCA
      841 TTGGAACGCG GATTCCCCGT GCCAAGAGTG ACgtaagtac cgcctataga gtctataggc
      901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gCTCCTGGGC
     1261 AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG AATTGGGatt cgaacatcga
     1321 ttGAATTCaA AGCTTgccgc cctgcgggag cggcccggcC TGGAGTTCGC CGAGGGCCGG
     1381 GCCGGCTGCG CCAGAGGCGG CGGCGGCTCT GGCGGAGGAG GAGGAGGAGG GGGAGGGGGA
     1441 GGAGGAGGTG GAGGAGGAGG CGGGGGAGGT GGAGGAGGAG GGGGAGGAGG GGGAGGTGGG
     1501 GGAGGAGGAG GGGGGGGAGG AGGAGGCGGG GGGGGAGGGG GAGGAGGTGG AGGAGGGGGG
     1561 GGAGGGGGCG GAGGAGGAGG AGGCGGAGGC GGAGGGGGAG GAGGAGGGGG CGGGGGAGGC
     1621 GGAGGAGGGG GGGGGGGAGG CGGGGGGGGC GGGGGAGGCG GCGGGGGAGG AGGGGGCGGG
     1681 GGGGGCGGCG GCGGCGCCGA CCTGCTGGTG ACCCCCCTGC TGGCCGCCCA CGCCGCCGGC
     1741 ACAGGCGCCG CCGAGAAGGG CGCCCCTAGC TCCGGCAAGG GATCCGTCAG CAAAGGCGAG
     1801 GAGCTTTTCA CCGGGGTTGT TCCCATCCTC GTCGAGCTCG ACGGCGACGT AAACGGCCAC
     1861 AAATTCAGCG TTTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT CACCCTCAAG
     1921 TTCATCTGCA CCACCGGCAA GCTCCCCGTC CCCTGGCCCA CCCTCGTCAC CACCCTCACC
     1981 TACGGCGTCC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
     2041 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
     2101 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
     2161 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
     2221 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
     2281 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
     2341 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
     2401 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     2461 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGt gataaGAATT Ctgcagatat
     2521 ccagcacagt ggcggccgCT CGAGTACCCA TACGATGTTC CAGATTACGC TAAGtaaAGA
     2581 TCtacgggtg gcatcCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA
     2641 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG
     2701 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA
     2761 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT
     2821 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG
     2881 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA
     2941 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC
     3001 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC
     3061 TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA
     3121 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC
     3181 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA
     3241 GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG
     3301 TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT
     3361 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC
     3421 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT
     3481 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT
     3541 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC
     3601 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC
     3661 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG
     3721 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC
     3781 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA
     3841 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG
     3901 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA
     3961 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT
     4021 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
     4081 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
     4141 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
     4201 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
     4261 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
     4321 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
     4381 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
     4441 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
     4501 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
     4561 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
     4621 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
     4681 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
     4741 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
     4801 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
     4861 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
     4921 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
     4981 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
     5041 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
     5101 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
     5161 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
     5221 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
     5281 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT
     5341 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
     5401 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
     5461 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
     5521 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
     5581 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
     5641 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
     5701 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
     5761 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
     5821 TGGCCTTTTG CTCACATGT
//

