LOCUS       pPR-1_1A_5T_AGGT        2157 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  D1.
ACCESSION   urn.local...9c-j14b1z1
VERSION     urn.local...9c-j14b1z1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..136
                     /Source="referenceToDocument: urn:local:.:1vj-j14b6sx"
                     /label="pPR-1_1A_5T_AGGT (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1..8
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        1..4
                     /label="Ligation"
     overhang        9..12
                     /created_by="farle"
                     /label="AGGT"
     source          <9..>121
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     CDS             10..129
                     /created_by="farle"
                     /label="CDS"
     motif           22..63
                     /created_by="farle"
                     /label="N-terminal Solvating Helix"
     source          <22..>121
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     protein_bind    76..78
                     /created_by="farle"
                     /modified_by="farle"
                     /modified_by="farle"
                     /label="T"
     source          <122..>128
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <122..>128
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        129..132
                     /created_by="farle"
                     /modified_by="farle"
                     /label="ACTC"
     misc_feature    133..4
                     /Source="referenceToDocument: urn:local:.:1vi-j14b6sx"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        133..136
                     /label="Ligation"
     source          join(2152..>4,<133..2151)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(169..187)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        255..359
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             360..1154
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1325..1913
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1814..1833
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2067..2084
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatctcaag gtggtaattc tgaggagccc agaaagtcat ttgatgagcg tcctgaaaga
       61 ggcgttgttt cttggaccgc tatgatctct ggatacgctc agaacggtcg tattgatgaa
      121 gcaagagaac tcttgtcttc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
      181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
      421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
      481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
      541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
      601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
      661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
      721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
      781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
      841 aggacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
      901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
      961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     1021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     1081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     1141 atgaattgtt ttagctgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtgaag
//
