LOCUS       pPR-1_1E_LN5T           2136 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  H1.
ACCESSION   urn.local...9q-j14b1z2
VERSION     urn.local...9q-j14b1z2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..115
                     /Source="referenceToDocument: urn:local:.:1x3-j14b7ee"
                     /label="pPR-1_1E_LN5T (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        5..8
                     /created_by="farle"
                     /label="TGAA"
     source          <5..>107
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>8
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             6..104
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     source          <9..>32
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <9..>32
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     protein_bind    33..35
                     /created_by="farle"
                     /label="N"
     source          <36..>101
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <36..>101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     protein_bind    48..50
                     /created_by="farle"
                     /label="T"
     overhang        104..107
                     /created_by="farle"
                     /label="CTTC"
     misc_feature    112..4
                     /Source="referenceToDocument: urn:local:.:1x2-j14b7ee"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        112..115
                     /label="Ligation"
     source          join(2131..>4,<112..2130)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(148..166)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        234..338
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             339..1133
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1304..1892
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1793..1812
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2046..2063
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acattgaact tttcgacaaa atgccagagc gtaacgttgt ttcttggacc gcaatgatct
       61 ctggatatgc acagaacggt agaatcgacg aagctagaga actcttccga gttgtcttca
      121 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      181 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      241 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      301 cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
      361 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
      421 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
      481 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
      541 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
      601 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
      661 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
      721 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
      781 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
      841 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
      901 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
      961 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     1021 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     1081 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
     1141 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1201 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1261 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1321 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1381 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1441 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1501 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1561 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1621 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1681 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1741 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1801 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1861 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1921 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1981 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2041 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2101 ccgcgcgttg gccgattcat taatcactct gtgaag
//
