LOCUS       pPR-1_2A_LD5T           2127 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  B2.
ACCESSION   urn.local...9k-j14b1z1
VERSION     urn.local...9k-j14b1z1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..106
                     /Source="referenceToDocument: urn:local:.:1wf-j14b73d"
                     /label="pPR-1_2A_LD5T (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        9..12
                     /created_by="farle"
                     /label="CTTC"
     source          <9..>95
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <9..>95
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             10..99
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    31..33
                     /created_by="farle"
                     /modified_by="farle"
                     /modified_by="farle"
                     /label="D"
     protein_bind    46..48
                     /created_by="farle"
                     /modified_by="farle"
                     /modified_by="farle"
                     /label="T"
     source          <96..>98
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <96..>98
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        99..102
                     /created_by="farle"
                     /label="ACTC"
     misc_feature    103..4
                     /Source="referenceToDocument: urn:local:.:1we-j14b73d"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        103..106
                     /label="Ligation"
     source          join(2122..>4,<103..2121)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(139..157)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        225..329
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             330..1124
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1295..1883
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1784..1803
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2037..2054
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatctcact tcgataaaat gccagagagg gatgttgttt cttggaccgc gatgatctct
       61 ggttatgcac agaacggtag gatagacgaa gcaagagaac tcttgtcttc acgaagtggc
      121 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      181 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      301 tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga
      361 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      421 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      481 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      541 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      601 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      661 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      721 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      781 tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc
      841 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      901 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
      961 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1021 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1081 gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt
     1141 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1201 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1441 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1981 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2041 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2101 ggccgattca ttaatcactc tgtgaag
//
