LOCUS       pPR-1_2E_LD             2073 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  E2.
ACCESSION   urn.local...99-j14b1yz
VERSION     urn.local...99-j14b1yz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..52
                     /Source="referenceToDocument: urn:local:.:1v7-j14b6of"
                     /label="pPR-1_2E_LD (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     CDS             5..41
                     /Original_Translation="MGNSVFSWNSMIRGYARNGQPEEALSLYSQMRRSG
                     IKPDNYTFPFVLKACASLSSLKEGKQIHGHVIKSGFESDVYVQSALIDMYAKCGELED
                     ARKVFDEMPERNVVSWNAMISGYAQNGQSEEALELFREMQQEGIKPSEFTFCSVLSAC
                     ASLGSLEMGKQIHGYVIKSGFESIVFVGNALIDMYAKCGSIEDARKVFDEMPERTVVS
                     WTAMISGYAQNGQSEEALELFREMQREGVKPDEVTLPSVLSACANLGALEQGKQIHAY
                     VIKSGFESDVFVGSALIDMYAKCGSIEDARKVFDKMPERDVVSWNAMISGYAMHGHGK
                     EALELFEEMQQSGIKPSHVTFTGVLSACSHAGLVDEGRQYFNSMKKDYGIEPRVEHYG
                     CMVDLLGRAGRLDEAYEFIESMPIEPNAVVWGALLGACRIHGNVELGERAAEKLFELE
                     PESSGNYVLLSNIYASAGRWDDVAKVRKMMKERGIKKEPGCSWIEVKNKVHEFVAGDR
                     SHPQSEEIYAKLEELSEKMKEAGYVPDTSFVLHDVEEEEKEQMLSYHSEKLAIAFGLI
                     STPPGTPIRIVKNLRVCGDCHTAIKFISKIVGREIIVRDSNRFHHFKDGSCSCGDYW"
                     /modified_by="farle"
                     /modified_by="farle"
                     /codon_start=2
                     /label="CDS"
     overhang        5..8
                     /created_by="farle"
                     /label="TGAA"
     source          <5..>34
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>34
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     source          <9..>32
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <9..>32
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     protein_bind    33..35
                     /created_by="farle"
                     /modified_by="farle"
                     /label="D"
     overhang        41..44
                     /created_by="farle"
                     /label="TTCG"
     misc_feature    49..4
                     /Source="referenceToDocument: urn:local:.:1v6-j14b6of"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        49..52
                     /label="Ligation"
     source          join(2068..>4,<49..2067)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(85..103)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        171..275
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             276..1070
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1241..1829
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1730..1749
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     1983..2000
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acattgaact tttcgacaaa atgccagagc gtgatgttgt ttcgcgagtt gtcttcacga
       61 agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      121 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      241 gataaatgct tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg
      301 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
      361 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
      421 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
      481 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
      541 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
      601 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
      661 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
      721 acgatctggc cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc
      781 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
      841 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
      901 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
      961 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     1021 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2041 cgcgttggcc gattcattaa tcactctgtg aag
//
