LOCUS       pPR-1_14A_LN5N          2134 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  A3.
ACCESSION   urn.local...b1-j14b1z7
VERSION     urn.local...b1-j14b1z7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..113
                     /Source="referenceToDocument: urn:local:.:22b-j14b883"
                     /label="pPR-1_14A_LN5N (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        9..12
                     /created_by="farle"
                     /label="GTGA"
     source          <9..>97
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <9..>97
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             11..106
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    38..40
                     /created_by="farle"
                     /label="N"
     protein_bind    53..55
                     /created_by="farle"
                     /label="N"
     source          98
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          98
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     source          <99..>105
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <99..>105
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        106..109
                     /created_by="farle"
                     /label="ACTC"
     misc_feature    110..4
                     /Source="referenceToDocument: urn:local:.:22a-j14b883"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        110..113
                     /label="Ligation"
     source          join(2129..>4,<110..2128)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(146..164)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        232..336
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             337..1131
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1302..1890
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1791..1810
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2044..2061
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatctcagt gaactcttcg ataaaatgcc agagaggaat gttgtttctt ggaatgcgat
       61 gatctctggt tatgcacaga acggtaggat agacgaagca agagaactct tgtcttcacg
      121 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
      181 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
      241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      301 tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg
      361 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
      421 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
      481 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
      541 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
      601 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
      661 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
      721 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
      781 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg
      841 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
      901 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
      961 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     1021 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     1081 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac
     1141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1921 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1981 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2041 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2101 gcgcgttggc cgattcatta atcactctgt gaag
//
