LOCUS       pPR-1_14E_LD5N          2129 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  C3.
ACCESSION   urn.local...b5-j14b1z8
VERSION     urn.local...b5-j14b1z8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..108
                     /Source="referenceToDocument: urn:local:.:22r-j14b8c7"
                     /label="pPR-1_14E_LD5N (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     source          <5..>100
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>100
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        5..8
                     /created_by="farle"
                     /label="TGAA"
     CDS             6..98
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    33..35
                     /created_by="farle"
                     /label="D"
     protein_bind    48..50
                     /created_by="farle"
                     /label="N"
     overhang        97..100
                     /created_by="farle"
                     /label="GTGA"
     misc_feature    105..4
                     /Source="referenceToDocument: urn:local:.:22q-j14b8c7"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        105..108
                     /label="Ligation"
     source          join(2124..>4,<105..2123)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(141..159)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        227..331
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             332..1126
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1297..1885
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1786..1805
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2039..2056
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acattgaatt gttcgataag atgccagaga gagatgttgt ttcttggaac gcaatgatct
       61 ctggatacgc acagaatggt aggatagatg aagcacgtga cgagttgtct tcacgaagtg
      121 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      181 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      301 aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt
      361 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      421 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      481 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      541 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      601 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      661 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      721 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      781 tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag atccgcgcga
      841 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
      901 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
      961 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1021 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1081 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt cagaccaagt
     1141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1441 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2101 ttggccgatt cattaatcac tctgtgaag
//
