LOCUS       pPR-1_19A_LD5T          2137 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  H3.
ACCESSION   urn.local...ax-j14b1z7
VERSION     urn.local...ax-j14b1z7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..116
                     /Source="referenceToDocument: urn:local:.:21v-j14b847"
                     /label="pPR-1_19A_LD5T (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        9..12
                     /created_by="farle"
                     /label="CACG"
     source          <9..>100
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <9..>100
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             11..109
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    41..43
                     /created_by="farle"
                     /label="D"
     protein_bind    56..58
                     /created_by="farle"
                     /label="T"
     source          101
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     source          <102..>108
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <102..>108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        109..112
                     /created_by="farle"
                     /label="ACTC"
     misc_feature    113..4
                     /Source="referenceToDocument: urn:local:.:21u-j14b847"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        113..116
                     /label="Ligation"
     source          join(2132..>4,<113..2131)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(149..167)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        235..339
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             340..1134
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1305..1893
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1794..1813
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2047..2064
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatctcaca cgtgaactct tcgataaaat gccagagagg gatgttgttt cttggaccgc
       61 gatgatctct ggttatgcac agaacggtag gatagacgaa gcaagagaac tcttgtcttc
      121 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
      181 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      241 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      301 ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca
      361 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
      421 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
      481 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
      541 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
      601 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
      661 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
      721 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
      781 aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat
      841 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
      901 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
      961 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     1021 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     1081 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca
     1141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1441 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1921 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1981 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2041 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2101 cccgcgcgtt ggccgattca ttaatcactc tgtgaag
//
