LOCUS       pPR-1_19E_LD5N          2126 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  C4.
ACCESSION   urn.local...b4-j14b1z8
VERSION     urn.local...b4-j14b1z8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>10,<108..2126)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    7..111
                     /Source="referenceToDocument: urn:local:.:22n-j14b8b7"
                     /label="pPR-1_19E_LD5N (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        7..10
                     /label="Ligation"
     overhang        11..14
                     /created_by="farle"
                     /label="TGAA"
     source          <11..>104
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <11..>104
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             12..101
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    39..41
                     /created_by="farle"
                     /label="D"
     protein_bind    54..56
                     /created_by="farle"
                     /label="N"
     overhang        100..103
                     /created_by="farle"
                     /label="CACG"
     misc_feature    108..10
                     /Source="referenceToDocument: urn:local:.:22m-j14b8b7"
                     /label="pAGM1311 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        108..111
                     /label="Ligation"
     primer_bind     complement(144..162)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        230..334
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             335..1129
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1300..1888
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1789..1808
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2042..2059
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 gtgaagacat tgaattgttc gataagatgc cagagagaga tgttgtttct tggaacgcaa
       61 tgatctctgg atacgcacag aatggtagga tagatgaagc acgcgagttg tcttcacgaa
      121 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      181 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      301 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
      361 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
      421 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
      481 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
      541 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
      601 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
      661 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
      721 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
      781 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
      841 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
      901 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
      961 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     1021 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     1081 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
     1141 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1201 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1441 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1921 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1981 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2041 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2101 gcgttggccg attcattaat cactct
//
