LOCUS       pPR-1_19E_LN5N          2126 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  E4.
ACCESSION   urn.local...b8-j14b1z8
VERSION     urn.local...b8-j14b1z8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..105
                     /Source="referenceToDocument: urn:local:.:233-j14b8f4"
                     /label="pPR-1_19E_LN5N (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     source          <5..>97
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>97
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        5..8
                     /created_by="farle"
                     /label="TGAA"
     CDS             6..95
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    33..35
                     /created_by="farle"
                     /label="N"
     protein_bind    48..50
                     /created_by="farle"
                     /label="N"
     overhang        94..97
                     /created_by="farle"
                     /label="CACG"
     misc_feature    102..4
                     /Source="referenceToDocument: urn:local:.:232-j14b8f4"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        102..105
                     /label="Ligation"
     source          join(2121..>4,<102..2120)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(138..156)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        224..328
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             329..1123
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1294..1882
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1783..1802
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2036..2053
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acattgaatt gttcgataag atgccagaga gaaacgttgt ttcttggaac gcaatgatct
       61 ctggatacgc acagaatggt aggatagatg aagcacgcga gttgtcttca cgaagtggct
      121 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      181 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      301 gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac cggaattgaa
      361 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      421 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      481 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      541 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      601 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      661 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      721 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      781 ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc cgcgcgagct
      841 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      901 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
      961 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1021 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1081 ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag accaagttta
     1141 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1201 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1261 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1321 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1381 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1441 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1501 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1561 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1621 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1681 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1741 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1801 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1861 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1921 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1981 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2041 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2101 gccgattcat taatcactct gtgaag
//
