LOCUS       pPR-1_B_LD5N            2120 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  G4.
ACCESSION   urn.local...9b-j14b1yz
VERSION     urn.local...9b-j14b1yz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..99
                     /Source="referenceToDocument: urn:local:.:1vf-j14b6qq"
                     /label="pPR-1_B_LD5N (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        5..8
                     /created_by="farle"
                     /label="ACTC"
     source          <5..>95
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>95
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             6..92
                     /Original_Translation="MGNSVFSWNSMIRGYARNGQPEEALSLYSQMRRSG
                     IKPDNYTFPFVLKACASLSSLKEGKQIHGHVIKSGFESDVYVQSALIDMYAKCGELED
                     ARKVFDEMPERNVVSWNAMISGYAQNGQSEEALELFREMQQEGIKPSEFTFCSVLSAC
                     ASLGSLEMGKQIHGYVIKSGFESIVFVGNALIDMYAKCGSIEDARKVFDEMPERTVVS
                     WTAMISGYAQNGQSEEALELFREMQREGVKPDEVTLPSVLSACANLGALEQGKQIHAY
                     VIKSGFESDVFVGSALIDMYAKCGSIEDARKVFDKMPERDVVSWNAMISGYAMHGHGK
                     EALELFEEMQQSGIKPSHVTFTGVLSACSHAGLVDEGRQYFNSMKKDYGIEPRVEHYG
                     CMVDLLGRAGRLDEAYEFIESMPIEPNAVVWGALLGACRIHGNVELGERAAEKLFELE
                     PESSGNYVLLSNIYASAGRWDDVAKVRKMMKERGIKKEPGCSWIEVKNKVHEFVAGDR
                     SHPQSEEIYAKLEELSEKMKEAGYVPDTSFVLHDVEEEEKEQMLSYHSEKLAIAFGLI
                     STPPGTPIRIVKNLRVCGDCHTAIKFISKIVGREIIVRDSNRFHHFKDGSCSCGDYW"
                     /modified_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    30..32
                     /created_by="farle"
                     /modified_by="farle"
                     /modified_by="farle"
                     /label="D"
     protein_bind    45..47
                     /created_by="farle"
                     /modified_by="farle"
                     /label="N"
     overhang        92..95
                     /created_by="farle"
                     /label="AAGA"
     misc_feature    96..4
                     /Source="referenceToDocument: urn:local:.:1ve-j14b6qq"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        96..99
                     /label="Ligation"
     source          join(2115..>4,<96..2114)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(132..150)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        218..322
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             323..1117
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1288..1876
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1777..1796
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2030..2047
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatactctt cgataagatg cctgaaagag atgttgtttc ttggaacgca atgatctctg
       61 gttacgctca gaacggtagg atcgatgagg caagattgtc ttcacgaagt ggctcttcag
      121 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      181 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      241 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      301 ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact
      361 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
      421 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
      481 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
      541 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
      601 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
      661 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
      721 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
      781 tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga
      841 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
      901 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
      961 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     1021 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     1081 aataaaatat tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata
     1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2101 tcattaatca ctctgtgaag
//
