LOCUS       pPR-1_C_LN5T            2124 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  F5.
ACCESSION   urn.local...9p-j14b1z2
VERSION     urn.local...9p-j14b1z2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..103
                     /Source="referenceToDocument: urn:local:.:1wz-j14b7aw"
                     /label="pPR-1_C_LN5T (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     overhang        5..8
                     /created_by="farle"
                     /label="AAGA"
     source          <5..>99
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>99
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             6..95
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     protein_bind    36..38
                     /created_by="farle"
                     /label="N"
     protein_bind    51..53
                     /created_by="farle"
                     /label="T"
     overhang        96..99
                     /created_by="farle"
                     /label="GCAC"
     misc_feature    100..4
                     /Source="referenceToDocument: urn:local:.:1wy-j14b7aw"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        100..103
                     /label="Ligation"
     source          join(2119..>4,<100..2118)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(136..154)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        222..326
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             327..1121
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1292..1880
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1781..1800
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2034..2051
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acataagaga gctgttcgac aagatgccag aacgcaatgt agtgtcgtgg acggctatga
       61 tatccgggta tgcacagaat ggaagaatag acgaagcact tgtcttcacg aagtggctct
      121 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      181 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa
      361 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
      421 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
      481 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
      541 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
      601 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
      661 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
      721 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
      781 ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt
      841 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
      901 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
      961 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     1021 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1081 agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac caagtttact
     1141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1981 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2041 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2101 cgattcatta atcactctgt gaag
//
