LOCUS       pPR-1_D_LD5T            2131 bp    DNA     circular UNA 11-FEB-2025
DEFINITION  H5.
ACCESSION   urn.local...9a-j14b1yz
VERSION     urn.local...9a-j14b1yz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..110
                     /Source="referenceToDocument: urn:local:.:1vb-j14b6pn"
                     /label="pPR-1_D_LD5T (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     CDS             5..103
                     /created_by="farle"
                     /modified_by="farle"
                     /label="CDS"
     source          <5..>106
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     source          <5..>106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     overhang        5..8
                     /created_by="farle"
                     /label="GCAC"
     protein_bind    38..40
                     /created_by="farle"
                     /label="D"
     protein_bind    53..55
                     /created_by="farle"
                     /label="T"
     overhang        103..106
                     /created_by="farle"
                     /label="TGAA"
     misc_feature    107..4
                     /Source="referenceToDocument: urn:local:.:1va-j14b6pn"
                     /label="pAGM1311 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        107..110
                     /label="Ligation"
     source          join(2126..>4,<107..2125)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(143..161)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     promoter        229..333
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             334..1128
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="KanR"
     rep_origin      1299..1887
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1788..1807
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2041..2058
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 acatgcacgg gagctttttg ataagatgcc tgaacgagat gtggtgtcct ggacagcaat
       61 gatctcgggg tatgctcaaa acggtcggat agacgaagca cgtgaattgt cttcacgaag
      121 tggctcttca gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      181 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      301 taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa
      361 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
      421 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
      481 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
      541 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
      601 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
      661 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
      721 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
      781 gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc
      841 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
      901 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
      961 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
     1021 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     1081 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa
     1141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1921 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1981 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2041 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2101 cgttggccga ttcattaatc actctgtgaa g
//
