LOCUS       pT2HE_BEPA_MYH3C2-C3        9235 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  BEPA_MYH3_Cint.
ACCESSION   BEPA_MYH3_Cint
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     regulatory      1012..1031
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T3 promoter"
     misc_feature    1066..1265
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Tol2 left"
     misc_structure  1248..1582
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    complement(1676..1873)
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 late polyA"
     misc_feature    complement(1892..2608)
                     /note="Geneious type: ORF"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="eGFP"
     regulatory      complement(2627..4143)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="zebrafish hsp70 promoter"
     primer_bind     4193..4210
                     /standard_name="MYH3C3_8kb_R"
     misc_feature    complement(4193..6984)
                     /standard_name="MYH3C_BEPA_insert"
     primer_bind     complement(6967..6984)
                     /standard_name="MYH3C3_8kb_F"
     misc_structure  6985..7331
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    complement(7371..7519)
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Tol2 right"
     misc_feature    8205..9062
                     /note="Geneious type: ORF"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      9220..604
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgcagag gtgtaaagta
     1081 cttgagtaat tttacttgat tactgtactt aagtattatt tttggggatt tttactttac
     1141 ttgagtacaa ttaaaaatca atacttttac ttttacttaa ttacattttt ttagaaaaaa
     1201 aagtactttt tactccttac aattttattt acagtcaaaa agtacttatt ttttggagat
     1261 cacttcattc tattttccct tgctattacc aaaccaattg aattgcgctg atgcccagtt
     1321 taatttaaat gttatttatt ctgcctatga aaatcgtttt cacattatat gaaattggtc
     1381 agacatgttc attggtcctt tggaagtgac gtcatgtcac atctattacc acaatgcaca
     1441 gcaccttgac ctggaaatta gggaaattat aacagtcaat cagtggaaga aaatggagga
     1501 agtatgtgat tcatcagcag ctgcgagcag cacagtccaa aatcagccac aggatcaaga
     1561 gcacccgtgg ccgtatcttc gcgaattctt ttctttaagt ggtgtaaata aagattcatt
     1621 caagatctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1681 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1861 tcatgtctgg atcatcatcg atgtcgcttt acttgtacag ctcgtccatg ccgagagtga
     1921 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     1981 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     2041 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     2101 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     2161 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     2221 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     2281 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     2341 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     2401 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     2461 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     2521 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     2581 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgt cgactgcagg aaaaaaaaac
     2641 aattagaatt aattttatat ttatacaatt tatggtgcaa ttgtttcatt atgaaaggat
     2701 ttttctagtc aacaagtgaa attctcttaa taaccaagtt attattagtt gtacaagtaa
     2761 aacgctaatt ttcttttgta gacaacatta acacaaattc atttaggaca tttgctagta
     2821 tttatggtta ttatgctcat ataaaataat gtcagcaaca taaagtaaat gcccgtctgt
     2881 tcattgcttt ttttaaaaat ccttattgtg aaaacgggaa atataatttt ctagtagacc
     2941 taaaataaga tgaacatagg cagtccacat gtaaaaagat tacatataca aagttgattt
     3001 tttgacatta agtacatata atctctatat aattctgatt agccaataga gtacaatata
     3061 ttgagactta ccttacagta gattcttaaa tagagcgaat aaacacctcg tcggggaaaa
     3121 agtcctgtca ggctgctctt ttcttctttg tcaagtcgct tctcttcggt tgagtttctc
     3181 tgcggtctct ctttcgccct ttaaatacag cagagcgcgc acccgttcag actctacact
     3241 gttctggaag cctccagtga attcgaccaa tcaaaacaca gcaggcagcc gcgtcacttc
     3301 ttcagctgtc attgagagga gccgctgaga ggaatgtagt ggagatttcc agagttttac
     3361 cgtgaaatta cgcgaaacag cggtcaaaaa tacaaagaga tttaaaatgt tttttttatt
     3421 tgagtgtttt attgtactta ttgctgaaat atgtgcggtt gtgtgatgtg ttgtttttcc
     3481 agttgcaatg aatgaacaaa ctaaaatgat aaaatgtttg aacgattcag taaaaaaaaa
     3541 tgcaataaaa aaatcttgct gaaatgtttt acacagtagc aaaatcgggc ctgtggtgtt
     3601 ttcagctaca tagaaggcaa acctgtttta tataagctaa tagctttggt cagatttgtt
     3661 taacgtttca acaaaacaaa tggatgttta ctgtactata ttgattggtt aaaaccaata
     3721 ctttattctg ggattgcagt cttaactatt tttgtagtgt taaaaacaac ttgctaactt
     3781 gagaggtcat gtttaccctc agagaaatag gcacaagtct ctttgtttaa tattggagat
     3841 catcctcctt gttcagtcgt ggtctgtatt taattttaat gtatatgatt gccgtaaaag
     3901 tgttagaaag tttaccctgg tgctatgaaa tcaatctgct gcttttgacc cagttagtgt
     3961 gtctttaatc taacttaatg agaccccagt gtggtcaaca atcaatagaa acaaagtgtg
     4021 ttgttttata actgttaact cctattattt tttatcttct gtagcatata gacaaaacat
     4081 gctcgttcta atagtttaat aaaattaatt tgatttttgg aaataaccaa gcgacacccc
     4141 tgaaggatcc cccggtgata tcgggcccgc tagcgtacgt aaggggccta tccgtactca
     4201 tggtggagcc ttatgtctgt taaataatta cattgggact gcaatgacta tagaatgact
     4261 ttacttgaaa tacattaaca aatcaacata tccttgtatg gttaccaata ggttttaact
     4321 ttacaactac gctaactcaa gtcttgcatt tgaatgttac agagaataca tttgaacatg
     4381 ttgagtttta ccaaaatctt tgaatggaaa accacagatc ttaaatggat cagaaacttt
     4441 ttgcatatgt ttcaaatgct tgcagagaaa aatcatgaat tgaatagtca tccttaccta
     4501 acaccttctc tcgtgacaat attccccgat cctgataaaa agagttgtgt tgttagagta
     4561 agtttttaaa aatatgcatg aaaatttgtc attgcttcta tttgcataag tatttgttaa
     4621 aaaagagaac atattcaaat gatcccaatt ttaagagaag aacgtattac cagaggcagg
     4681 aactcaggtc ttcagtctca caacttgtcc tgtccttgag ttgcctttat actgaatctt
     4741 gttttgatga cacagtaaaa gttaaatatg gtacaccaaa tgtggtaagg cagacggccc
     4801 ttgataaaag ataggttgta attatatcac gtgatgtttc aggttacagt ttatatggcc
     4861 gggggtttat cattacttca acagaagttt attttaggct cttgactgtc tctccactct
     4921 aaagaaggta aagtcatttt aaaaagtcct gtgtttctta attgtttttt ttttttattt
     4981 gcagaatctt actgcgctat ttaaataaaa taacttgact tcatttcaaa atataaggca
     5041 ctatagtact aaacaagcac attaaatatc tgaaatgtaa gaaactattg atgcagaaaa
     5101 agagatcatc aattttaaaa caaagagttg tacaacacag gacgctctct gttaaaagat
     5161 cctatgaaag taatttaact cattttttac aaaatggggg gccagattcg ttttgatcac
     5221 ggtcaaaagt cagtgcttct tgaatgcttt aggtatttga actgtgtaat tcaaacgtat
     5281 tggcatccaa agatggtttc aaagtaaaaa tatatattta agagacttgg aggacacaac
     5341 tagataaaaa caacacagac aatgcaagtc gagatcaaaa actactttat tattggggat
     5401 ttcacatcat attttatgat tagaaaaaaa catcagccaa agcactactc agctgcctct
     5461 tttccctgta gaaaaaacag acaatgaata aatatacttc atatgcacca agatgcatat
     5521 catactttgt cattgttttg tcctaattct atgaacctat ttttttaatt catgactaaa
     5581 tgttaagtga aaatcattaa aaaggaattt aattgtattc aatgttcatt aagctgaatg
     5641 tcagttacct tgccagagtc acggcttttt gctctcagct tgttgacctg ggactctgca
     5701 atgtctgcac gctcctcagc ctcctccagc tcgtgctgga ccttcctgca cttggacaga
     5761 tgagcattgg cctgttcctc ctggaaaaca agcaaaaaaa atcatacata caaaaaaata
     5821 tatatataaa aatatctttg gaaaagaccc agtggtccag tgttcatgaa atacttatga
     5881 catagtagtc attttgcttt aaaaaaaagc aaaaattcag tccttaccgc ctcctcagcc
     5941 tgcctcttgt aagccttcac cttcagttgc aacttgtcaa ccagatcttg cagccttgag
     6001 acatttttct tgtcctcttc agtctgtaat agaaggtgtt gaggtgcaaa tatacatttt
     6061 tgaaatggta acagtaatgg agaatttggt ctttctttat tttttagtga ctgtacctga
     6121 taggtgagct ccttcactct cctctcatat ttgcggacac ccttaacagc atctgctcca
     6181 cgtctctgct cagcctcaac ctctgtctca agctcacgca ccttaaaaaa aaaagagaaa
     6241 atgaatgatt ttccgtattg tttcaaagcc ttgatttagc tgtacaaatc atgagaggac
     6301 tttcttaccc tggactccag tttctggagc tgcttcttgc cacccttcat ggccaggttc
     6361 tcagcctcgt ccaggcggtg ctgcaggtcc ttaacagcga cctccagatt cttcttcatc
     6421 ctctcaaggt gagcactagt gtcttgctcc ttcttcagct cttcagccat catagcagcc
     6481 tggaggagac atcataacat gttgtttgac aaaaactaac aaagtacatt tgagtaaaag
     6541 gaacaacaat agaattcaac cctacatcag tgatggcctt cttggccttc tcttctgcat
     6601 tccttgcttc ctgaacagtg tcatccactt caccctggac ctggaccagg tcagtctcaa
     6661 gcttcttctt ggtgttcatg agacttgtgt tctaaaagat acaacaattt taatcacatg
     6721 tcacattgtt acattatctt aaatgtcttt ataccgttta attattgaag aagttaagga
     6781 agtttgttca cctgagagtg caggagtcca acacgctcac tggcgtccac caactcctgc
     6841 tccgcgattt tgcggcttct ctctgtctgt tccagagcag ctctaagttc ctcaatttca
     6901 gccatcatga gaccgttcct gcgatccacc atggcagctt gttccttgaa gtcttcctgg
     6961 gctctaacag catcgtcaag gtgcatctgg cctgtgtttc agacaccagg gagtctctgc
     7021 tcacgtttcc tgctatttgc agcctctcta tcaagactaa tacacctctt cccgcatcgg
     7081 ctgcctgtga gaggcttttc agcactgcag gattgctttt cagccccaaa agagctaggc
     7141 ttgacactaa caattttgag aatcagcttc tactgaagtt aaatctgagg ttttacaact
     7201 ttgagtagcg tgtactggca ttagattgtc tgtcttatag tttgataatt aaatacaaac
     7261 agttctaaag caggataaaa ccttgtatgc atttcattta atgttttttg agattaaaag
     7321 cttaaacaag aatctctagt tttctttctt gcttttactt ttacttcctt aatactcaag
     7381 tacaatttta atggagtact tttttacttt tactcaagta agattctagc cagatacttt
     7441 tacttttaat tgagtaaaat tttccctaag tacttgtact ttcacttgag taaaattttt
     7501 gagtactttt tacacctctg tcaagaactc ctggacaaac ctctgacctg tgtggaacag
     7561 agtggatatg ggtgtctgaa cagatattca cgtcttttgc agatcagagg gcatttctgg
     7621 tgttgtgcaa tgacctgggt ccaacaggta ggcgtactcg tatccaagag cattgttgga
     7681 caggaggtag tctccattcc tataaagagg aaggaatggc accatgtagt agccgtcatt
     7741 gtggccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8041 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
