LOCUS       pGLU_446                5621 bp ds-DNA     circular     03-SEP-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     miscellaneous   1..30
                     /label="Himar_LE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   complement(1..30)
                     /label="Himar_RE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     prokaryote t... complement(31..116)
                     /label="TermC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(121..855)
                     /label="ermC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     prokaryote p... complement(857..1308)
                     /label="PermC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1033..1068
                     /label="oLO.284"
                     /note="sequence: CATGATGAGTGATCGTTAAATTTATACTGCAATCGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1309..1325
                     /label="oCG0036"
                     /note="sequence: GGGTCACGCGTAGGACG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1309..1326)
                     /label="JL.0061"
                     /note="sequence: ACATACCGACCCACCCTCATGCGTCCTACGCGTGACCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Primer site     1309..1338
                     /label="F-skpp-asm-15-mer-2x"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1309..1338
                     /label="F primer binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Barcode         1343..1362
                     /label="BC.0003"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     Barcode         1343..1362
                     /label=">skpp-3-F sk20mer-83381"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Primer site     1386..1415
                     /label="R-skpp-asm-15-mer-2x"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1386..1415)
                     /label="R primer binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1395..1415)
                     /label="oLO.170"
                     /note="sequence: CCAGCTTCACACGGCGTGTGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1398..1415
                     /label="JL.0062"
                     /note="sequence: TCGCNNNNNNNNNNNNNNNNNNNNCGGAAGTGCTCCACCTGTGACGATCACGCCGTGTGAAGCTGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1399..1415)
                     /label="JL0073_TnVar-Arb_JL33trunc7_rev"
                     /note="sequence: CCAGCTTCACACGGCGT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     miscellaneous   1416..1445
                     /label="Himar_RE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   complement(1416..1445)
                     /label="Himar_LE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      1446..1506
                     /label="L3S2P21"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1453..1478)
                     /label="oLO.285"
                     /note="sequence: CGGGAGGCCTCTTTTCTGGAATTTGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Promoter        1507..1930
                     /label="p19296"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1931..2977
                     /label="Himar.004"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 2982..3162
                     /label="TAmarelleT9"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3163..3188
                     /label="oLO.254"
                     /note="sequence: CTTTTCCGCTGCATAACCCTGCTTCG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     miscellaneous   3163..3408
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     miscellaneous   complement(3417..3456)
                     /label="UNS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     prokaryote t... complement(3457..3486)
                     /label="L2U5H11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3534..3556)
                     /label="oLO.286"
                     /note="sequence: TGTAAAACGACGGCCAGTCATGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3539..3555)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3539..3556)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3548..3570)
                     /label="M13/pUC Forward"
                     /note="sequence: CCCAGTCACGACGTTGTAAAACG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        3669..3773
                     /label="Pamp"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3774..4634
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     4735..4755
                     /label="attB1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4735..4755
                     /label="Gateway recomb1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     ORI             complement(4891..5279)
                     /label="R6K ORI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          5450..5472
                     /label="M13/pUC Reverse"
                     /note="sequence: AGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     5469..5485
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     5469..5489
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     prokaryote p... complement(5542..5573)
                     /label="L2U8H11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     miscellaneous   complement(5574..5613)
                     /label="UNSX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 TAACAGGTTG GATGATAAGT CCCCGGTCTA GCCGACTGCG CAAAAGACAT AATCGATTCA
       61 CAAAAAATAG GCACACGAAA AACAAGTTAA GGGATGCAGT TTATGCATCC CTTAACAAGC
      121 TTACTTATTA AATAATTTAT AGCTATTGAA AAGAGATAAG AATTGTTCAA AGCTAATATT
      181 GTTTAAATCG TCAATTCCTG CATGTTTTAA GGAATTGTTA AATTGATTTT TTGTAAATAT
      241 TTTCTTGTAT TCTTTGTTAA CCCATTTCAT AACGAAATAA TTATACTTTT GTTTATCTTT
      301 GTGTGATATT CTTGATTTTT TTCTACTTAA TCTGATAAGT GAGCTATTCA CTTTAGGTTT
      361 AGGATGAAAA TATTCTCTTG GAACCATACT TAATATAGAA ATATCAACTT CTGCCATTAA
      421 AAGTAATGCC AATGAGCGTT TTGTATTTAA TAATCTTTTA GCAAACCCGT ATTCCACGAT
      481 TAAATAAATC TCATTAGCTA TACTATCAAA AACAATTTTG CGTATTATAT CCGTACTTAT
      541 GTTATAAGGT ATATTACCAT ATATTTTATA GGATTGGTTT TTAGGAAATT TAAACTGCAA
      601 TATATCCTTG TTTAAAACTT GGAAATTATC GTGATCAACA AGTTTATTTT CTGTAGTTTT
      661 GCATAATTTA TGGTCTATTT CAATGGCAGT TACGAAATTA CACCTCTTTA CTAATTCAAG
      721 GGTAAAATGG CCTTTTCCTG AGCCGATTTC AAAGATATTA TCATGTTCAT TTAATCTTAT
      781 GTTTGTCATA ATCTTGTCGA TATTATGCTT AGAAGTAATA AAGTTTTGAC TGTGTTTTAT
      841 ATTTTTCTCG TTCATTTATA ACCCTCTTTA ATTTGGTTAT ATGAATTTTG CTTATTAACG
      901 ATTCATTATA ACCACTTATT TTTTGTTTGG TTGATAATGA ACTGTGCTGA TTACAAAAAT
      961 ACTAAAAATG CCCATATTTT TTCCTCCTTA TAAAATTAGT ATAATTATAG CACGACCTCT
     1021 GATAAATATG AACATGATGA GTGATCGTTA AATTTATACT GCAATCGGAT GCGATTATTG
     1081 AATAAAAGAT ATGAGAGATT TATCTAATTT CTTTTTTCTT GTAAAAAAAG AAAGTTCTTA
     1141 AAGGTTTTAT AGTTTTGGTC GTAGAGCACA CGGTTTAACG ACTTAATTAC GAAGTAAATA
     1201 AGTCTAGTGT GTTAGACTTT ATGAAATCTA TATACGTTTA TATATATTTA TTATCCGGAG
     1261 GTGTAGCATG TCTCATTCAA TTTTGAGGGT TGCCAGAGTT AAAGGATCGG GTCACGCGTA
     1321 GGACGCGTCG AGTAGGGTAA TGTTGGTCAT GTGCTTTTCG TTGCATACAT GCTCCTCTGG
     1381 AAGTTGTTCC GCAGCCACAC GCCGTGTGAA GCTGGTAGAC CGGGGACTTA TCATCCAACC
     1441 TGTTACTCGG TACCAAATTC CAGAAAAGAG GCCTCCCGAA AGGGGGGCCT TTTTTCGTTT
     1501 TGGTCCTTTG AATAAAAAAA CTTGAAAGAA ACTGACCATG CATTCCCCTC CTAAAGCTTT
     1561 ATTTTGGGAG GGAAAGCGGT CTTTTTTGTT TTATTATTTT GTCCATGTGG TCTGATGAAG
     1621 AATAAAGTAG TGGAAATAGG ACTGCCAGGC AAAAAGAAAC ATAACATATC TTGTCTTTTC
     1681 AAAACCTTGG TTTTTGCATC TTCTTCTTGA AAAGCTTATG ATAGAGAGAA GGGGCATTTT
     1741 TCAAAATATT AATTTGTTTT GTGAAGATGT CTTTGTTAAG ATTAAAAACA GCACAGTTAT
     1801 CGATCGTACT TTATCATAGA ATAAACTTAT AATGGCAATG GGATTTTTGT TTACTTAGGA
     1861 AATACTTTTC TTTTAAGTGA GTCTTATTTA CTAAAGACAC TAAAGTCCCG ATTTGAAAGG
     1921 AGCTTTACAT ATGGAAAAAA AGGAATTTCG TGTATTAATC AAATACTGTT TTTTGAAGGG
     1981 CAAAAATACG GTAGAAGCGA AAACTTGGCT AGATAATGAG TTTCCCGACT CGGCACCTGG
     2041 GAAATCTACG ATCATCGATT GGTATGCGAA ATTCAAGCGT GGTGAAATGT CGACCGAGGA
     2101 CGGTGAAAGA TCTGGCAGAC CCAAAGAGGT AGTAACGGAC GAAAACATCA AAAAAATCCA
     2161 CAAAATGATC TTAAATGACC GTAAAATGAA GTTAATCGAG ATCGCGGAGG CACTTAAGAT
     2221 CTCTAAGGAA CGTGTAGGTC ATATCATCCA TCAATATTTA GATATGAGGA AGCTCTGTGC
     2281 CAAATGGGTA CCCAGAGAGC TCACGTTTGA CCAAAAACAA CGCCGTGTAG ATGATTCGAA
     2341 GAGGTGTTTA CAGTTGCTTA CTCGTAATAC GCCTGAGTTT TTCCGTCGCT ATGTAACGAT
     2401 GGATGAAACG TGGCTCCATC ACTACACTCC GGAGTCTAAT CGCCAGTCGG CGGAGTGGAC
     2461 GGCCACGGGT GAACCCTCGC CCAAGCGTGG CAAGACTCAA AAGTCTGCGG GTAAAGTGAT
     2521 GGCATCGGTA TTTTGGGATG CCCATGGCAT CATCTTTATC GATTATCTAG AGAAGGGCAA
     2581 AACGATCAAC TCTGACTATT ATATGGCCCT TTTAGAGCGT TTAAAGGTCG AAATCGCCGC
     2641 GAAAAGGCCT CACATGAAGA AGAAAAAAGT ATTATTCCAC CAAGACAACG CGCCCTGCCA
     2701 CAAGTCTTTA CGAACCATGG CGAAAATCCA TGAATTAGGT TTCGAATTAC TACCTCACCC
     2761 CCCCTATTCG CCTGATTTGG CACCTTCGGA CTTTTTCTTA TTCTCTGACC TCAAACGGAT
     2821 GCTCGCGGGG AAAAAATTTG GTTGCAATGA AGAGGTAATC GCAGAAACTG AGGCATATTT
     2881 TGAGGCGAAA CCCAAGGAGT ACTACCAAAA TGGTATCAAA AAATTAGAAG GTCGTTATAA
     2941 TCGTTGTATC GCGCTAGAAG GGAACTATGT AGAATAAGCT TGGAATCTCC TTCTCGCCTC
     3001 TTTCGCTGAA CACGAAAGCG AATCCGTTCA GCACACACTT TACGATATGG CGCAAAAAGT
     3061 CCTCGGCTGC GTGCCGGAAG TGAAAGACAT CCACCTCACC ATGCCCAACA AGCATTGCCT
     3121 GCTCGTGGAC CTGTCCCGCT TCGGTCAGGA CAATCCCAAC GACTTTTCCG CTGCATAACC
     3181 CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC
     3241 AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG
     3301 CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC
     3361 GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGTACT CCTTCGGAGA
     3421 CGAGACGAGA CAGCCTGAGA ATGGATGCGA GTAATGATAC AACAATAGCG TGCTGTTCGC
     3481 ACGCTAAGGT CAGGCGGAAT GGCACTTCCT TATTCCAGAT GCGTGCGGAT TATGCATGAC
     3541 TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGCTTGTGGT GGATCACAGT
     3601 TATTGGAGAC TTCCTTGGCC GTACGATGGG TTTTGTTTCA GGTATCTGGC ACTTTTCGGG
     3661 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
     3721 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
     3781 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
     3841 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
     3901 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
     3961 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG
     4021 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
     4081 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
     4141 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
     4201 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
     4261 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG
     4321 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
     4381 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
     4441 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGC TCTCGCGGTA
     4501 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
     4561 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
     4621 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
     4681 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTGA ATTGCCATTA TCAACAAGTT
     4741 TGTACAAAAA AGCAGGCTCC GAATTGGTAT CACGAGGACG ATTTGAAGCC CCCTACCCAT
     4801 TTACGCTGAC TTGACGGGAC GGCGGCTTTG TTGAATAGAG CGTCAGACCC CGTAGAAAAG
     4861 ATCAAAGGAT CTTCGGCCGG CCGGCGCGCC GATCTGAAGA TCAGCAGTTC AACCTGTTGA
     4921 TAGTACGTAC TAAGCTCTCA TGTTTCACGT ACTAAGCTCT CATGTTTAAC GTACTAAGCT
     4981 CTCATGTTTA ACGAACTAAA CCCTCATGGC TAACGTACTA AGCTCTCATG GCTAACGTAC
     5041 TAAGCTCTCA TGTTTCACGT ACTAAGCTCT CATGTTTGAA CAATAAAATT AATATAAATC
     5101 AGCAACTTAA ATAGCCTCTA AGGTTTTAAG TTTTATAAGA AAAAAAAGAA TATATAAGGC
     5161 TTTTAAAGCC TTTAAGGTTT AACGGTTGTG GACAACAAGC CAGGGATGTA ACGCACTGAG
     5221 AAGCCCTTAG AGCCTCTCAA AGCAATTTTG AGTGACACAG GAACACTTAA CGGCTGACAT
     5281 GGGGCGCGCC CAGCTGTAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
     5341 GGCCTTTTGC TCACATGTTC TTTCCAGCGA CATATGCCAT ACGCCGAACT GAACTCCACC
     5401 TAATGTCTTG GAGATTGTCG GATAGCGCGG AATAGGATTG TGCCCTCTGA GCGGATAACA
     5461 ATTTCACACA GGAAACAGCT ATGACCATGA CACATGCATC CACCATCGCA GACTTATCAT
     5521 CACTGAGCCT CCACCTAGCC TATACAACAA TAGCGTGCGT GGTTAGCACG CTAGGTGGAA
     5581 GGGCTCGGAG TTGTGGTAAT CTATGTATCC TGGGGTTAGC G
//