LOCUS       pTE5601-SUMO-dark        6130 bp    DNA     circular UNA 18-NOV-2025
DEFINITION  T7-SUMO-dark peptide to inhibit 11S nanoluc fragment.
ACCESSION   -
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             140..997
                     /note="Bla.pep"
                     /label="Bla.pep"
     CDS             complement(3376..4455)
                     /note="lacI.pep"
                     /label="lacI.pep"
     primer_bind     4625..4646
                     /%GC="59.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="24.6"
                     /Tm="66.0"
                     /annotation_group="67638434205005ea9fbf5-d5ce-4c58-85b6-0f
                     980cdeaa39"
                     /created_by="primer3"
                     /Sequence="TGGTGCATGCAAGGAGATGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pMM-012_SUMO-dark"
                     /label="p137_pETbck_F"
     primer_bind     4625..4646
                     /%GC="59.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="24.6"
                     /Tm="66.0"
                     /annotation_group="881783796514004afb7920-f09b-4d46-bd94-2
                     ddb7ca00f01"
                     /created_by="primer3"
                     /Sequence="TGGTGCATGCAAGGAGATGGCG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="p137_pETbck_F"
     primer_bind     4800..4814
                     /%GC="66.7"
                     /Hairpin_Tm="47.9"
                     /Self_Dimer_Tm="17.4"
                     /Tm="55.9"
                     /annotation_group="698428900200037a869c3-c350-439c-9678-a0
                     e580a15c02"
                     /created_by="primer3"
                     /Sequence="ATGCGTCCGGCGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pMM-012_SUMO-dark"
                     /label="pET_up-Microsynth_Std"
     primer_bind     4800..4814
                     /%GC="66.7"
                     /Hairpin_Tm="47.9"
                     /Self_Dimer_Tm="17.4"
                     /Tm="55.9"
                     /annotation_group="88178105451700e3a32386-df2a-4b2f-8b7b-7
                     dce351b73ad"
                     /created_by="primer3"
                     /Sequence="ATGCGTCCGGCGTAG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="pET_up-Microsynth_Std"
     misc_feature    4842..4859
                     /note="PT7"
     primer_bind     complement(4861..4883)
                     /%GC="39.1"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="4.1"
                     /Tm="57.8"
                     /annotation_group="6763932600000ab85c2f3-b3f2-46ab-92c5-82
                     1895d82207"
                     /created_by="primer3"
                     /Sequence="AATTGTTATCCGCTCACAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pMM-012_SUMO-dark"
                     /label="p151_Ptac_R"
     primer_bind     complement(4861..4883)
                     /%GC="39.1"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="4.1"
                     /Tm="57.8"
                     /annotation_group="881743110037008d17e1a6-4755-4180-aa62-f
                     95b09ab93a4"
                     /created_by="primer3"
                     /Sequence="AATTGTTATCCGCTCACAATTCC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="p151_Ptac_R"
     misc_feature    4862..4884
                     /note="lacO"
     primer_bind     4898..4923
                     /%GC="23.1"
                     /Hairpin_Tm="37.2"
                     /Self_Dimer_Tm="None"
                     /Tm="53.9"
                     /annotation_group="6764027762800b3b3e3ef-eb4b-4602-a74e-bc
                     5eee9dd0fd"
                     /created_by="primer3"
                     /Sequence="ATTTTGTTTAACTTTAAGAAGGAGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pMM-012_SUMO-dark"
                     /label="p261_pET28_RBS_F"
     primer_bind     4898..4923
                     /%GC="23.1"
                     /Hairpin_Tm="37.2"
                     /Self_Dimer_Tm="None"
                     /Tm="53.9"
                     /annotation_group="88177222876600e26deed8-0504-411c-ba73-b
                     72cb89f874a"
                     /created_by="primer3"
                     /Sequence="ATTTTGTTTAACTTTAAGAAGGAGAT"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="p261_pET28_RBS_F"
     primer_bind     complement(4903..4928)
                     /%GC="30.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="53.8"
                     /annotation_group="67641291034007ecc3c9e-55e1-4934-856b-42
                     9d92d27e47"
                     /created_by="primer3"
                     /Sequence="GGTATATCTCCTTCTTAAAGTTAAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pMM-012_SUMO-dark"
                     /label="p262_pET28_RBS_R"
     primer_bind     complement(4903..4928)
                     /%GC="30.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="53.8"
                     /annotation_group="881786557446006a1e2d18-3940-4667-8016-3
                     95a8be83e41"
                     /created_by="primer3"
                     /Sequence="GGTATATCTCCTTCTTAAAGTTAAAC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="p262_pET28_RBS_R"
     RBS             4916..4921
                     /note="rbs"
     CDS             4929..5303
                     /note="His6-SUMO-?.pep"
                     /codon_start=1
                     /transl_table=1
                     /Original_Translation="MRGSHHHHHHMASGSDSEVNQEAKPEVKPEVKPET
                     HINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPED
                     LDMEDNDIIEAHREQIGGRRAELRRQACGRTRARVTAGRGSGC*"
                     /label="His6-SUMO-?.pep"
     misc_feature    4959..4962
                     /note="T7tag.pep"
     CDS             4971..5261
                     /note="SUMO"
                     /modified_by="MeierM"
                     /label="SUMO (SMT3_yeast)"
     primer_bind     complement(5239..5261)
                     /Sequence="ACCACCAATCTGTTCTCTGTGAG"
                     /Tm="60.2"
                     /%GC="47.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="KueffnerA"
                     /label="SUMO_Vector_reverse"
     primer_bind     complement(5243..5290)
                     /created_by="MeierM"
                     /Sequence="TTAAACAGAGCCCAGCCGCTCACGCCGGAACCACCAATCTGTTCTCT
                     G"
                     /Tm="73.1"
                     /%GC="56.3"
                     /Hairpin_Tm="50.2"
                     /Self_Dimer_Tm="20.6"
                     /modified_by="MeierM"
                     /label="oMM-007_SUMO-drk-rv"
     primer_bind     complement(5243..5290)
                     /%GC="56.3"
                     /Hairpin_Tm="50.2"
                     /Self_Dimer_Tm="20.6"
                     /Tm="73.1"
                     /annotation_group="881778097344000464b797-341f-47e6-a2c8-2
                     4a56a3a901b"
                     /Sequence="TTAAACAGAGCCCAGCCGCTCACGCCGGAACCACCAATCTGTTCTCT
                     G"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="oMM-007_SUMO-drk-rv"
     primer_bind     complement(5247..5267)
                     /created_by="primer3"
                     /Tm_(binding_region)="61.8"
                     /Tm_(with_extension)="79.6"
                     /%GC_(binding_region)="57.1"
                     /%GC_(with_extension)="66.7"
                     /Hairpin_Tm_(with_extension)="62.7"
                     /Self_Dimer_Tm_(with_extension)="46.6"
                     /annotation_group="881751607898004c37eac4-d5a5-4487-aee6-7
                     61b1b1edf80"
                     /Sequence="GCCGGAACCACCAATCTGTTC"
                     /Mismatches=0
                     /Extension="GCAGCCCGGGCAGCA"
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="oMM-063-SUMO-TC-pep99-R"
     primer_bind     complement(5250..5267)
                     /created_by="primer3"
                     /Tm_(binding_region)="58.4"
                     /Tm_(with_extension)="75.8"
                     /%GC_(binding_region)="61.1"
                     /%GC_(with_extension)="69.0"
                     /Hairpin_Tm_(with_extension)="62.7"
                     /Self_Dimer_Tm_(with_extension)="26.1"
                     /annotation_group="88176339463000e0e7850c-ba20-47a3-81d1-8
                     8b80a6896ec"
                     /Sequence="GCCGGAACCACCAATCTG"
                     /Mismatches=0
                     /Extension="CCCGGGCAGCA"
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="oMM-065-SUMO-TC-STOP-R"
     misc_feature    <5262..>5300
                     /Source="referenceToDocument: urn:local:MeierM:x6-fn5r3c6"
                     /label="lepB-strep_T7space PCR Product (concatenated
                     sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <5262..>5300
                     /Source="referenceToDocument: urn:local:MeierM:y-isvre8d"
                     /label="pMM-005-lepB_delta2-75-Strep_T7 PCR Product
                     extraction (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             5268..>5300
                     /created_by="MeierM"
                     /modified_by="MeierM"
                     /label="dark peptide (HiBiT)"
     primer_bind     5274..5320
                     /created_by="MeierM"
                     /Sequence="GGCTGGGCTCTGTTTAAAAAAATTAGCTAACAAAGCCCGAAAGGAAG
                     "
                     /Tm="66.5"
                     /%GC="42.6"
                     /Hairpin_Tm="51.7"
                     /Self_Dimer_Tm="24.6"
                     /modified_by="MeierM"
                     /label="oMM-006_SUMO-dark-fw"
     primer_bind     5274..5320
                     /%GC="42.6"
                     /Hairpin_Tm="51.7"
                     /Self_Dimer_Tm="24.6"
                     /Tm="66.5"
                     /annotation_group="8817486552670065d3c803-13aa-4eb6-9ca0-c
                     030ba42392b"
                     /Sequence="GGCTGGGCTCTGTTTAAAAAAATTAGCTAACAAAGCCCGAAAGGAAG
                     "
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="oMM-006_SUMO-dark-fw"
     misc_feature    5280..5282
                     /created_by="MeierM"
                     /label="R->A for dark peptide"
     primer_bind     5304..5321
                     /created_by="primer3"
                     /Tm_(binding_region)="56.7"
                     /Tm_(with_extension)="68.4"
                     /%GC_(binding_region)="55.6"
                     /%GC_(with_extension)="53.6"
                     /Hairpin_Tm_(with_extension)="53.0"
                     /Self_Dimer_Tm_(with_extension)="None"
                     /annotation_group="8817573341730097636f9b-0c63-4816-9d45-3
                     3a5d4fad477"
                     /Sequence="CAAAGCCCGAAAGGAAGC"
                     /Mismatches=0
                     /Extension="CTGCTGCTAA"
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="oMM-064-SUMO-TC-STOP-F"
     primer_bind     complement(5339..5358)
                     /%GC="50.0"
                     /Hairpin_Tm="35.0"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /annotation_group="881789145298004015647b-c54a-4dfc-8571-b
                     6aa4d621f96"
                     /created_by="primer3"
                     /Sequence="TGCTAGTTATTGCTCAGCGG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="T7term_MicrosynthStd"
     misc_feature    5354..5400
                     /note="TT7"
     primer_bind     complement(5783..5800)
                     /%GC="33.3"
                     /Hairpin_Tm="31.5"
                     /Self_Dimer_Tm="None"
                     /Tm="43.9"
                     /annotation_group="88176908070100cac40e43-947a-4c59-b24c-9
                     1f30371134e"
                     /created_by="primer3"
                     /Sequence="GATTTAATCTGTATCAGG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pMM-012_SUMO-dark"
                     /label="p84_pKOV_bck_F"
ORIGIN      
        1 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt
       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      301 ccttgagagt tttcgccccg aagaacgttc tccaatgatg agcactttta aagttctgct
      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
     2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcacttgatg
     2821 cctccgtgta agggggaatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     2881 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     2941 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     3001 ccgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
     3061 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     3121 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     3181 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     3241 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     3301 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     3361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     3481 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     3541 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     3601 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc
     3661 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     3721 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     3781 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     3841 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     3901 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     3961 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     4021 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     4081 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     4141 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     4201 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     4261 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     4321 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     4381 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     4441 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     4501 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     4561 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     4621 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     4681 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     4741 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     4801 tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg
     4861 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
     4921 gatataccat gcgtggttct caccaccacc accaccatat ggctagcgga tcggactcag
     4981 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca
     5041 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt
     5101 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat
     5161 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg
     5221 ataacgatat tattgaggct cacagagaac agattggtgg ttccggcgtg agcggctggg
     5281 ctctgtttaa aaaaattagc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     5341 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     5401 ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca
     5461 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag
     5521 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa
     5581 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     5641 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     5701 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcagctt ggctgttttg
     5761 gcggatgaga taagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     5821 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     5881 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     5941 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     6001 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     6061 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     6121 caaattaagc
//
