LOCUS       pTE5608-SecA            6618 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  SecA - motor protein for post-translational translocation.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.1.
FEATURES             Location/Qualifiers
     rep_origin      1..429
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     CDS             complement(548..1363)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="KanR"
     rep_origin      1485..2073
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             complement(2500..2691)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="rop"
     promoter        3633..3651
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     misc_feature    3653..3676
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 5' UTR
                     spacer (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5'
                     UTR spacer (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5'
                     UTR spacer (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5'
                     UTR spacer (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /label="5' UTR spacer"
     misc_feature    3677..3697
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B0030
                     RBS (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     B0030 RBS (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     B0030 RBS (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     B0030 RBS (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /label="B0030 RBS"
     CDS             3698..6400
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="secA"
     gene            3698..6403
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5608-SAS435-secA_T7space"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EG10936
                     (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /label="EG10936"
     terminator      6404..6465
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; L3S2P21
                     (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:p-isvre86"">pTE5602-pMM-020-T7-pro
                     OmpA-pep99</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:q-isvre87"">pTE5603-pMM-031-T7-PR(
                     -1tm)-pep104</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /label="L3S2P21"
     terminator      6479..6607
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:p-isvre86"">pTE5602-pMM-020-T7-pro
                     OmpA-pep99</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:q-isvre87"">pTE5603-pMM-031-T7-PR(
                     -1tm)-pep104</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:56/-25;
                     T7 terminator (<a
                     href=""urn:local:MeierM:j-jwuyh4e"">251113ONT-SAS453-scott
                     </a>)"
                     /label="T7_terminator"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
      541 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
      601 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
      661 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
      721 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
      781 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
      841 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
      901 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
      961 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     1021 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     1081 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1141 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     1201 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1261 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1321 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     1381 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
     1441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1621 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2281 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     2341 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     2401 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2461 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2521 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     2581 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     2641 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctaatgcctc
     2701 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     2761 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     2821 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     2881 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     2941 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     3001 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     3061 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     3121 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat
     3181 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     3241 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     3301 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     3361 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     3421 tgggttggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     3481 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     3541 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     3601 atgcgtccgg cgtagaggat cgagatctcg ggtaatacga ctcactatag ggcgagtaga
     3661 aataattttg tttaagatta aagaggagaa attacatatg ctaatcaaat tgttaactaa
     3721 agttttcggt agtcgtaacg atcgcaccct gcgccggatg cgcaaagtgg tcaacatcat
     3781 caatgccatg gaaccggaga tggaaaaact ctccgacgaa gaactgaaag ggaaaaccgc
     3841 agagtttcgt gcacgtctgg aaaaaggcga agtgctggaa aatctgatcc cggaagcttt
     3901 cgccgtggta cgtgaggcaa gtaagcgcgt ctttggtatg cgtcacttcg acgttcagtt
     3961 actcggcggt atggttctta acgaacgctg catcgccgaa atgcgtaccg gtgaaggaaa
     4021 aaccctgacc gcaacgctgc ctgcttacct gaacgcacta accggtaaag gcgtgcacgt
     4081 agttaccgtc aacgactacc tggcgcaacg tgacgccgaa aacaaccgtc cgctgtttga
     4141 attccttggc ctgactgtcg gtatcaacct gccgggcatg ccagcaccgg caaagcgcga
     4201 agcttacgca gctgacatca cttacggtac gaacaacgaa tacggctttg actacctgcg
     4261 cgacaacatg gcgttcagcc ctgaagaacg tgtacagcgt aaactgcact atgcgctggt
     4321 ggacgaagtg gactccatcc tgatcgatga agcgcgtaca ccgctgatca tttccggccc
     4381 ggcagaagac agctcggaaa tgtataaacg cgtgaataaa attattccgc acctgatccg
     4441 tcaggaaaaa gaagactccg aaaccttcca gggcgaaggc cacttctcgg tggacgaaaa
     4501 atctcgccag gtgaacctga ccgaacgtgg tctggtgctg attgaagaac tgctggtgaa
     4561 agagggcatc atggatgaag gggagtctct gtactctccg gccaacatca tgctgatgca
     4621 ccacgtaacg gcggcgctgc gcgctcatgc gctgtttacc cgtgacgtcg actacatcgt
     4681 taaagatggt gaagttatca tcgttgacga acacaccggt cgtaccatgc agggccgtcg
     4741 ctggtccgat ggtctgcacc aggctgtgga agcgaaagaa ggtgtgcaga tccagaacga
     4801 aaaccaaacg ctggcttcga tcaccttcca gaactacttc cgtctgtatg aaaaactggc
     4861 ggggatgacc ggtactgctg ataccgaagc tttcgaattt agctcaatct acaagctgga
     4921 taccgtcgtt gttccgacca accgtccaat gattcgtaaa gatctgccgg acctggtcta
     4981 catgactgaa gcggaaaaaa ttcaggcgat cattgaagat atcaaagaac gtactgcgaa
     5041 aggccagccg gtgctggtgg gtactatctc catcgaaaaa tcggagctgg tgtcaaacga
     5101 actgaccaaa gccggtatta agcacaacgt cctgaacgcc aaattccacg ccaacgaagc
     5161 ggcgattgtt gctcaggcag gttatccggc tgcggtgact atcgcgacca atatggcggg
     5221 tcgtggtaca gatattgtgc tcggtggtag ctggcaggca gaagttgccg cgctggaaaa
     5281 tccgaccgca gagcaaattg aaaaaattaa agccgactgg caggtacgtc acgatgcggt
     5341 actggaagca ggtggcctgc atatcatcgg taccgagcgt cacgaatccc gtcgtatcga
     5401 taaccagttg cgcggtcgtt ctggtcgtca gggggatgct ggttcttccc gtttctacct
     5461 gtcgatggaa gatgcgctga tgcgtatttt tgcttccgac cgagtatccg gcatgatgcg
     5521 taaactgggt atgaagccag gcgaagccat tgaacacccg tgggtgacta aagcgattgc
     5581 caacgcccag cgtaaagttg aaagccgtaa cttcgacatt cgtaagcaac tgctggaata
     5641 tgatgacgtg gctaacgatc agcgtcgcgc catttactcc cagcgtaacg aactgttgga
     5701 tgtcagcgat gtgagcgaaa ccattaacag cattcgtgaa gatgtgttca aagcgaccat
     5761 tgatgcctac attccaccac agtcgctgga agaaatgtgg gatattccgg ggctgcagga
     5821 acgtctgaag aacgatttcg acctcgattt gccaattgcc gagtggctgg ataaagaacc
     5881 agaactgcat gaagagacgc tgcgtgagcg cattctggcg cagtccatcg aagtgtatca
     5941 gcgtaaagaa gaagtggttg gtgctgagat gatgcgtcac ttcgagaaag gcgtcatgct
     6001 gcaaacgctt gactccctgt ggaaagagca cctggcagcg atggactatc tgcgtcaggg
     6061 tatccacctg cgtggctacg cacagaaaga tccgaagcag gaatacaaac gtgaatcgtt
     6121 ctccatgttt gcagcgatgc tggagtcgtt gaaatatgaa gttatcagta cgctgagcaa
     6181 agttcaggta cgtatgcctg aagaggttga ggagctggaa caacagcgtc gtatggaagc
     6241 cgagcgttta gcgcaaatgc agcagcttag ccatcaggat gacgactctg cagccgcagc
     6301 tgcactggcg gcgcaaaccg gagagcgcaa agtaggacgt aacgatcctt gcccgtgcgg
     6361 ttctggtaaa aaatacaagc agtgccatgg ccgcctgcaa taacctcggt accaaattcc
     6421 agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtcccactg agatccggct
     6481 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     6541 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     6601 tccggattgg cgaatggg
//
