LOCUS       pTE5609-proOmpA-pep86        4486 bp ds-DNA     circular     05-FEB-2026
DEFINITION  proOmpA-pep86 reporter for translocation.
ACCESSION   urn.local.MeierM.j0-k86wewt
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(216..>3904,<1..215)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..62
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /X-Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:p-isvre86"">pTE5602-pMM-020-T7-pro
                     OmpA-pep99</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:q-isvre87"">pTE5603-pMM-031-T7-PR(
                     -1tm)-pep104</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5608-SecA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; L3S2P21
                     (<a
                     href=""urn:local:MeierM:11-jwuyufs"">pTE5608-SecA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L3S2P21 (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="L3S2P21"
     primer_bind     1..27
                     /Tm_(binding_region)="63.6"
                     /Tm_(with_extension)="72.2"
                     /%GC_(binding_region)="48.1"
                     /%GC_(with_extension)="50.9"
                     /Hairpin_Tm_(with_extension)="55.1"
                     /Self_Dimer_Tm_(with_extension)="17.5"
                     /Sequence="CCTCGGTACCAAATTCCAGAAAAGAGG"
                     /Mismatches=0
                     /Extension="GGTTCCGGTTGTTGTCCGGGTTGTTGTTGA"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5636-p(extCore)OAss-nanobody-HiBiT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-076-Open-pMM20-fw+TC-tag (<a
                     href=""urn:local:MeierM:1m-jx1knsk"">pTE5636-p(extCore)OAs
                     s-nanobody-HiBiT</a>)"
                     /label="oMM-076-Open-pMM20-fw+TC-tag"
     primer_bind     complement(1..20)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.6"
                     /Tm="56.9"
                     /created_by="primer3"
                     /Sequence="TCTGGAATTTGGTACCGAGG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p155_AHLdisp_R (<a
                     href=""urn:local:MeierM:1i-jx1knsk"">pTE5632-pTrbCss-nanob
                     ody-HiBiT </a>)"
                     /label="p155_AHLdisp_R"
     primer_bind     1..20
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.5"
                     /Tm="56.9"
                     /created_by="primer3"
                     /Sequence="CCTCGGTACCAAATTCCAGA"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p16_secBck_F (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p16_secBck_F"
     primer_bind     complement(43..88)
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="2.6"
                     /Tm="69.8"
                     /Sequence="GCTTTGTTAGCAGCCGGATCTCAGTGGGACCAAAACGAAAAAAGGC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p79_sec3'term_GApET_R (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p79_sec3'term_GApET_R"
     primer_bind     63..79
                     /%GC="64.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.4"
                     /created_by="primer3"
                     /Sequence="CACTGAGATCCGGCTGC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p80_pETbck_F (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p80_pETbck_F"
     terminator      76..204
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /X-Transferred_From="pTE5604-SAS358-T7-space-Y(P276R)EG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1d-isvrgiv"">pTE5604-SAS358-T7-spa
                     ce-Y(P276R)EG</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1e-isvrgiv"">pTE5607-SAS385-T7-spa
                     ce-YEG</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1f-isvrgiv"">pTE5608-SAS435-secA_T
                     7space</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:p-isvre86"">pTE5602-pMM-020-T7-pro
                     OmpA-pep99</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:q-isvre87"">pTE5603-pMM-031-T7-PR(
                     -1tm)-pep104</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:s-isvre87"">pTE5605-pMM-090-T7-spa
                     ce-secY(P84L)EG_lib#22</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:56/-25;
                     T7 terminator (<a
                     href=""urn:local:MeierM:j-jwuyh4e"">251113ONT-SAS453-scott
                     </a>)"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5608-SecA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:11-jwuyufs"">pTE5608-SecA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7_terminator (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="T7_terminator"
     primer_bind     83..100
                     /created_by="primer3"
                     /Tm_(binding_region)="56.7"
                     /Tm_(with_extension)="68.4"
                     /%GC_(binding_region)="55.6"
                     /%GC_(with_extension)="53.6"
                     /Hairpin_Tm_(with_extension)="53.0"
                     /Self_Dimer_Tm_(with_extension)="None"
                     /Sequence="CAAAGCCCGAAAGGAAGC"
                     /Mismatches=0
                     /Extension="CTGCTGCTAA"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5636-p(extCore)OAss-nanobody-HiBiT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-064-SUMO-TC-STOP-F (<a
                     href=""urn:local:MeierM:1m-jx1knsk"">pTE5636-p(extCore)OAs
                     s-nanobody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-064-SUMO-TC-STOP-F (<a
                     href=""urn:local:MeierM:o-isvre86"">pTE5601-SUMO-dark</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-064-SUMO-TC-STOP-F (<a
                     href=""urn:local:MeierM:r-isvre87"">pTE5604-MBP-dark</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-064-SUMO-TC-STOP-F (<a
                     href=""urn:local:MeierM:r-isvre87"">pTE5604-MBP-dark</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-064-SUMO-TC-STOP-F (<a
                     href=""urn:local:MeierM:r-isvre87"">pTE5604-MBP-dark</a>)"
                     /label="oMM-064-SUMO-TC-STOP-F"
     primer_bind     complement(118..137)
                     /%GC="50.0"
                     /Hairpin_Tm="35.0"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /created_by="primer3"
                     /Sequence="TGCTAGTTATTGCTCAGCGG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     T7term_MicrosynthStd (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /label="T7term_MicrosynthStd"
     primer_bind     complement(118..136)
                     /note="T7 terminator, reverse primer"
                     /label="T7 Term"
     misc_feature    133..179
                     /note="TT7"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5601-SUMO-dark"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TT7 (<a
                     href=""urn:local:MeierM:o-isvre86"">pTE5601-SUMO-dark</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TT7
                     (<a
                     href=""urn:local:MeierM:r-isvre87"">pTE5604-MBP-dark</a>)"
     primer_bind     complement(179..199)
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="53.2"
                     /created_by="primer3"
                     /Sequence="GATATAGTTCCTCCTTTCAGC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5636-p(extCore)OAss-nanobody-HiBiT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pGMP-rev-MicrSynthStd (<a
                     href=""urn:local:MeierM:1m-jx1knsk"">pTE5636-p(extCore)OAs
                     s-nanobody-HiBiT</a>)"
                     /label="pGMP-rev-MicrSynthStd"
     rep_origin      216..671
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(303..322)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     513..534
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     CDS             complement(763..1578)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin in bacteria or G418
                      (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="KanR"
     primer_bind     1486..1505
                     /note="Kanamycin resistance gene, reverse primer"
                     /label="Kan-R"
     primer_bind     1570..1589
                     /note="pENTR vectors, reverse primer"
                     /label="pENTR-R"
     primer_bind     1570..1589
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /created_by="primer3"
                     /Sequence="ATGGCTCATAACACCCCTTG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p103_SecBck_w.rpt_R (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p103_SecBck_w.rpt_R"
     rep_origin      1700..2288
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     2189..2208
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2442..2459
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     misc_feature    2474..2613
                     /note="basis of mobility region from pBR322"
                     /label="bom"
     primer_bind     complement(2539..2558)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     2699..2721
                     /note="pGEX vectors, reverse primer"
                     /label="pGEX 3'"
     misc_feature    complement(2715..2906)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ROP (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ROP
                     (<a
                     href=""urn:local:MeierM:1i-jx1knsk"">pTE5632-pTrbCss-nanob
                     ody-HiBiT </a>)"
                     /label="ROP"
     primer_bind     3641..3662
                     /%GC="59.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="24.6"
                     /Tm="66.0"
                     /created_by="primer3"
                     /Sequence="TGGTGCATGCAAGGAGATGGCG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p137_pETbck_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /label="p137_pETbck_F"
     primer_bind     3755..3774
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
                     /label="pBRrevBam"
     primer_bind     3816..3830
                     /%GC="66.7"
                     /Hairpin_Tm="47.9"
                     /Self_Dimer_Tm="17.4"
                     /Tm="55.9"
                     /created_by="primer3"
                     /Sequence="ATGCGTCCGGCGTAG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pET_up-Microsynth_Std (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /label="pET_up-Microsynth_Std"
     primer_bind     3836..3858
                     /%GC="52.2"
                     /Hairpin_Tm="48.2"
                     /Self_Dimer_Tm="32.8"
                     /Tm="59.9"
                     /created_by="primer3"
                     /Sequence="CGAGATCTCGGGTAATACGACTC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p220_lin4PURE_F (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p220_lin4PURE_F"
     primer_bind     3846..3890
                     /%GC="40.0"
                     /Hairpin_Tm="58.9"
                     /Self_Dimer_Tm="16.6"
                     /Tm="62.4"
                     /Sequence="GGTAATACGACTCACTATAGATACTCGCTAGGGCGAATACTATTA"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p265_RBS732_T7GA_F (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p265_RBS732_T7GA_F"
     promoter        3848..3865
                     /note="T7 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /X-Transferred_From="T7"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:181-iup4kyo"">T7</a>"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5620-proteorhodopsin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 (<a
                     href=""urn:local:MeierM:l-jx1k4rg"">pTE5620-proteorhodopsi
                     n</a>)"
                     /label="T7"
     misc_feature    3848..3904
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5'lacJctFrag (<a
                     href=""urn:local:MeierM:1i-jx1knsk"">pTE5632-pTrbCss-nanob
                     ody-HiBiT </a>)"
                     /label="5'lacJctFrag"
     misc_feature    3866..3904
                     /created_by="MeierM"
                     /modified_by="MeierM"
                     /label="RBS 723597 TIR for lacY"
     primer_bind     3871..3895
                     /%GC="44.0"
                     /Hairpin_Tm="62.1"
                     /Self_Dimer_Tm="4.3"
                     /Tm="58.8"
                     /created_by="primer3"
                     /Sequence="CGCTAGGGCGAATACTATTATAAGG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p222_sigmaRBS_F (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /label="p222_sigmaRBS_F"
     primer_bind     complement(3876..3904)
                     /created_by="primer3"
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="61.8"
                     /%GC_(binding_region)="37.9"
                     /%GC_(with_extension)="37.5"
                     /Hairpin_Tm_(with_extension)="31.5"
                     /Self_Dimer_Tm_(with_extension)="None"
                     /Sequence="AAAAGTCCTCCTTATAATAGTATTCGCCC"
                     /Mismatches=0
                     /Extension="CAT"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5636-p(extCore)OAss-nanobody-HiBiT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     oMM-077-Open-pMM20-rev (<a
                     href=""urn:local:MeierM:1m-jx1knsk"">pTE5636-p(extCore)OAs
                     s-nanobody-HiBiT</a>)"
                     /label="oMM-077-Open-pMM20-rev"
     primer_bind     complement(3879..3904)
                     /%GC="30.8"
                     /Hairpin_Tm="31.5"
                     /Self_Dimer_Tm="None"
                     /Tm="54.7"
                     /created_by="primer3"
                     /Sequence="AAAAGTCCTCCTTATAATAGTATTCG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTE5609-proOmpA-pep86"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:13-k86mt2h"">pTE5609-proOmpA-pep86
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:19-jx1knsk"">pTE5625-LepB-HiBiT</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:19-k86mt7h"">pTE5610-proOmpA-pep79
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1a-jx1knsk"">pTE5624-PR-pep104</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1b-jx1knsk"">pTE5621-PR(-2tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1c-jx1knsk"">pTE5622-PR(-3tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1d-jx1knsk"">pTE5623-PR(-4tm)-pep1
                     04</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1f-jx1knsk"">pTE5629-pYncJss-nanob
                     ody-HiBiT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p40_SecIndivBck_R (<a
                     href=""urn:local:MeierM:1g-jx1knsk"">pTE5630-pPpiAss-nanob
                     ody-HiBiT </a>)"
                     /label="p40_SecIndivBck_R"
     misc_feature    3905..4438
                     /created_by="MeierM"
                     /label="proOmpA aa1-178"
     misc_feature    4439..4450
                     /created_by="MeierM"
                     /label="GGSG linker"
     CDS             4451..4486
                     /created_by="MeierM"
                     /label="HiBiT"
ORIGIN      
        1 cctcggtacc aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt
       61 cccactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      121 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      181 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      241 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      301 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      361 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      421 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      481 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      541 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      601 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      661 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      721 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
      781 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
      841 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
      901 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
      961 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     1021 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     1081 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     1141 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     1201 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     1261 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     1321 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     1381 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     1441 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     1501 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
     1561 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     1621 tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2461 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2521 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     2581 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     2641 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2701 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     2761 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     2821 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     2881 agggcggttt tttcctgttt ggtcactaat gcctccgtgt aagggggatt tctgttcatg
     2941 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     3001 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     3061 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     3121 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     3181 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
     3241 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     3301 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     3361 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     3421 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     3481 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     3541 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
     3601 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tggtgcatgc aaggagatgg
     3661 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     3721 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     3781 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     3841 tctcgggtaa tacgactcac tatagatact cgctagggcg aatactatta taaggaggac
     3901 ttttatgaaa aagacagcta tcgcgattgc agtggcactg gctggtttcg ctaccgtagc
     3961 gcaggccgct ccgaaagata acacctggta cactggtgct aaactgggct ggtcccagta
     4021 ccatgacact ggtttcatca acaacaatgg cccgacccat gaaaaccaac tgggcgctgg
     4081 tgcttttggt ggttaccagg ttaacccgta tgttggcttt gaaatgggtt acgactggtt
     4141 aggtcgtatg ccgtacaaag gcagcgttga aaacggtgca tacaaagctc agggcgttca
     4201 actgaccgct aaactgggtt acccaatcac tgacgacctg gacatctaca ctcgtctggg
     4261 tggcatggta tggcgtgcag acactaaatc caacgtttat ggtaaaaacc acgacaccgg
     4321 cgtttctccg gtcttcgctg gcggtgttga gtacgcgatc actcctgaaa tcgctacccg
     4381 tctggaatac cagtggacca acaacatcgg tgacgcacac accatcggca ctcgtccggg
     4441 aggctccggc gtgagcggct ggcgcctgtt taaaaaaatt agctaa
//
