LOCUS       Exported                5398 bp ds-DNA     circular SYN 16-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 3, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     79..99
                     /label=HA_forw_kozak
                     /note="color: black; sequence: 
                     GGATCCCATCGATTCGccATGTACCCATACGATGTTCCAGATTACGC"
     misc_feature    79..94
                     /label=pCS2
                     /note="color: #a6acb3"
     misc_feature    93..100
                     /label=kozak
                     /note="color: #339966"
     CDS             97..162
                     /codon_start=1
                     /label=lambda N22 peptide
                     /note="color: #ffcc00"
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     primer_bind     163..216
                     /label=1141a-HA-Adar-forw
                     /note="color: black; sequence: 
                     TACCCATACGATGTTCCAGATTACGCTggcggaCAGCTGGACCAAACACCGTCC"
     misc_feature    163..189
                     /label=HA
                     /note="color: #0000ff"
     primer_bind     163..188
                     /label=HA_forw_kozak
                     /note="color: black; sequence: 
                     GGATCCCATCGATTCGccATGTACCCATACGATGTTCCAGATTACGC"
     misc_feature    196..1400
                     /label=Catalytic Site
                     /note="color: #ff6600; direction: RIGHT"
     CDS             196..1400
                     /codon_start=1
                     /label=adarb1a
                     /note="color: #993366"
                     /translation="QLDQTPSRQPIPREGLQLHLPQVLADAVSRLVVDKFSELTDNFSS
                     PHARRKVLAGVVMTTGKDVMDAQVICVTTGTKCINGEYMSDRGLALNDCHAEIIARRSL
                     IRYLYNQLEYFLSDSTEEHEKSIFRWCSEHGYRLKDDIQFHLYISTSPCGDARIFSPHE
                     AGAEDQGDRHPNRKARGQLRTKIESGQGTIPVRTSNTIQTWDGVLQGERLLTMSCSDKI
                     ARWNVIGVQGSLLSYFTEPIYFSSLILGSLYHADHLSRAMYQRIADMDDIPPPFRINRP
                     LLSGISNTEARQPGKAPNFSVNWTVGDQGLEIINATTGKDDLGRQSQLCKHALYSRWVC
                     LHAKLSETLRIRGSRPGSYHEAKQGAVEYHEAKQTLFKAFYKAGLGAWVEKPIEQDQFS
                     LT"
     primer_bind     196..212
                     /label=1111a
                     /note="color: black; sequence: 
                     GGCCCCCCCTCGAGGATGCAGCTGGACCAAACACC"
     primer_bind     196..212
                     /label=1101a
                     /note="color: black; sequence: ATGCAGCTGGACCAAACACC"
     CDS             763..765
                     /label=E488Q
                     /note="color: #00ccff; direction: RIGHT"
     primer_bind     complement(1374..1425)
                     /label=1141b-Adar1-Rev
                     /note="color: black; sequence: 
                     CTAGAGGCTCGAGAGGCCTTGCTAGGGAGTAAGGGAAAACTGGTCTTGTTCG"
     primer_bind     complement(1377..1401)
                     /label=1111b
                     /note="color: black; sequence: 
                     gggtacatggtggcGGGAGTAAGGGAAAACTGGTCTTGT"
     primer_bind     complement(1377..1400)
                     /label=1101b
                     /note="color: black; sequence: AGGAGTAAGGGAAAACTGGTCTTGT"
     misc_feature    1423..1450
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     polyA_signal    1450..1584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(1697..1715)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1736..1752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    1760..1776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(1784..1814)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1784 .. 1790 / #ffffff / -10
                        2: 1791 .. 1808 / #ffffff
                        3: 1809 .. 1814 / #ffffff / -35"
     rep_origin      complement(2138..2726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2897..3757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2897 .. 3688 / #ccffcc
                        2: 3689 .. 3757 / #ccffcc / signal sequence
                     Cleavage site after base 3688"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3758..3862)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3888..4343)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4417..5398
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgccatgg acgcgcagac gcggcgacgc
      121 gagcgccggg cagagaagca ggctcaatgg aaggcagcta actacccata cgatgttcca
      181 gattacgctg gcggacagct ggaccaaaca ccgtcccgac agccaatccc cagggaggga
      241 cttcagctgc atttgcccca ggtcctcgcg gatgcggtct ctcgtttagt tgtggataag
      301 ttcagcgagc tgacagacaa cttctcgtct ccacatgcac ggcggaaagt cctggcagga
      361 gttgtcatga caaccggtaa ggatgtgatg gacgctcagg tgatttgtgt gacgacggga
      421 accaagtgta taaacggaga gtacatgagc gatcgaggac tggcccttaa tgactgccat
      481 gctgagatca tcgcacggcg ctcactcatt agatacctgt acaatcagct ggaatacttc
      541 ctcagcgaca gcactgaaga acatgagaaa tccatattca gatggtgcag tgaacatggc
      601 taccggctaa aagatgatat tcaattccat ctgtatatca gcacgtcgcc ctgtggagac
      661 gcccggatct tctcccctca tgaggctggt gccgaggacc agggtgaccg acatccaaac
      721 cgcaaggccc gtggacaact acgaaccaag attgagtctg gacaaggaac gatacctgtt
      781 cgtacgagta acaccattca gacttgggat ggagtcctgc agggagagcg actgctaacc
      841 atgtcatgca gtgacaaaat tgccagatgg aacgtgatcg gagtacaggg atctcttctg
      901 agttacttca cagagcccat ctatttctcc agtttaattc tgggaagtct gtatcatgct
      961 gaccatcttt ccagagccat gtaccagcgc atcgcagaca tggatgatat tcccccgcca
     1021 ttcagaatca acagaccact tcttagcggt atcagtaaca cagaagcaag gcaacctggg
     1081 aaagctccta atttcagcgt caactggaca gtaggagacc aggggttgga gatcattaac
     1141 gcaaccacag gaaaagatga cctcggaaga cagtcgcaac tttgcaaaca cgctctttac
     1201 agtcgttggg tctgtctaca tgcaaagctc agtgagactc tgcggatccg aggatctcgg
     1261 cctggctcct accacgaggc aaaacaaggg gcggtcgagt atcatgaggc gaaacagact
     1321 cttttcaaag ctttctacaa agcgggactg ggagcatggg tggaaaaacc catcgaacaa
     1381 gaccagtttt cccttactcc ctagcaaggc ctctcgagcc tctagaacta tagtgagtcg
     1441 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     1501 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1561 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1621 cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg
     1681 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     1741 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga
     1801 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1861 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1921 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     3901 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3961 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4021 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4081 aaaaatcgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4141 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4201 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4261 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4321 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     4381 gcgatcggtg cgggcctctt cgctattacg ccagtcgacc gccaattcaa tatggcgtat
     4441 atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat
     4501 atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac
     4561 ttggcaccat gccaattcaa tatggcggac ctggcactgt gccaactggg gaggggtcta
     4621 cttggcacgg tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga
     4681 attggcatgg tgccaataat ggcggccata ttggctatat gccaggatca atatataggc
     4741 aatatccaat atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg
     4801 ccctatgcca tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca
     4861 atgggcggtc ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca
     4921 ttgacgtcaa tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt
     4981 gacgtatatg gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat
     5041 gcccattgac gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat
     5101 tcatatccgt tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt
     5161 acatcaatat ctattaatag taacttggca agtacattac tattggaagt acgccagggt
     5221 acattggcag tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt
     5281 acatccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta
     5341 aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
