LOCUS       Exported                5290 bp ds-DNA     circular SYN 22-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 3, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     76..154
                     /label=2093a_hsADAR2_TO_pCS2_F_EcoR1
                     /note="color: black; sequence: 
                     GCAGGATCCCATCGATTCGccaccATGTACCCATACGATGTTCCAGATTACGCTggagg
                     ccagctgcatttaccgcagg"
     primer_bind     95..129
                     /label=2305_PTB1_HA_Adar
                     /note="color: black; sequence: 
                     CCTTCTCCAAGTCCACCATCTACCCATACGATGTTCCAGATTACGCT; added: 
                     2023-04-11"
     primer_bind     98..128
                     /label=Aco1_Adar_F
                     /note="color: black; sequence: 
                     CATGATCCGCAAGATGGCCAAGTACCCATACGATGTTCCAGATTACGC; added: 
                     2023-05-22"
     CDS             100..129
                     /codon_start=1
                     /label=HA
                     /note="color: #993366"
                     /translation="MYPYDVPDYA"
     primer_bind     103..154
                     /label=2094a
                     /note="color: black; sequence: 
                     TACCCATACGATGTTCCAGATTACGCTggcggacagctgcatttaccgcagg; 
                     added: 2022-06-08"
     primer_bind     103..129
                     /label=2306_FMRP_HA_Adar
                     /note="color: black; sequence: 
                     CCACTCGTGAATGGAGTACCCTACCCATACGATGTTCCAGATTACGCT; added: 
                     2023-04-11"
     primer_bind     103..128
                     /label=2138c_EcoRV_HA
                     /note="color: black; sequence: 
                     ggaaggcagctaacgatatcTACCCATACGATGTTCCAGATTACGC; added: 
                     2022-07-22"
     primer_bind     103..128
                     /label=2304_Stau_HA_Adar
                     /note="color: black; sequence: 
                     GAAATCAAACTCGGCCGTCTACCCATACGATGTTCCAGATTACGC; added: 
                     2023-04-11"
     primer_bind     115..154
                     /label=2140
                     /note="color: black; sequence: 
                     GTTCCAGATTACGCTggatatcggaggccagctgcatttaccgcagg; added: 
                     2022-07-22"
     CDS             130..1293
                     /codon_start=1
                     /label=hsADAR2_E488Q_T375G
                     /note="color: #99cc00"
                     /translation="GGQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMT
                     TGTDVKDAKVISVSTGGKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNN
                     KDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKA
                     RGQLRTKIESGQGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSI
                     FVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGK
                     APNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKIT
                     KPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKSTEQDQFSLT"
     primer_bind     221..244
                     /label=2173a_hsAdar_R348E
                     /note="color: black; sequence: cccctcacgctgagagaaaagtgc; 
                     added: 2022-09-18"
     primer_bind     complement(221..244)
                     /label=2173b_hsAdar_R348E
                     /note="color: black; sequence: gcacttttctctcagcgtgagggg; 
                     added: 2022-09-18"
     primer_bind     230..251
                     /label=2172a_hsAdar_V351L
                     /note="color: black; sequence: ctcgcagaaaactgctggctgg; 
                     added: 2022-09-17"
     misc_feature    232..234
                     /label=R348E
                     /note="color: #ff0000"
     misc_feature    241..243
                     /label=V351L
                     /note="color: #ff0000"
     primer_bind     294..336
                     /label=2169a_hsAdar_G375T
                     /note="color: black; sequence: 
                     gataagtgtttctacaggaACAaaatgtattaatggtgaatac; added: 
                     2022-09-17"
     primer_bind     complement(294..336)
                     /label=2169b_hsAdar_G375T
                     /note="color: black; sequence: 
                     gtattcaccattaatacatttTGTtcctgtagaaacacttatc; added: 
                     2022-09-17"
     CDS             313..315
                     /label=T375G
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(544..566)
                     /label=2174b_hsAdar_R55G
                     /note="color: black; sequence: ggtgagaagattccggcatctcc; 
                     added: 2022-09-18"
     primer_bind     544..566
                     /label=2174a_hsAdar_R455G
                     /note="color: black; sequence: ggagatgccggaatcttctcacc; 
                     added: 2022-09-18"
     misc_feature    553..555
                     /label=R455G
                     /note="color: #ff0000"
     primer_bind     592..623
                     /label=2171a_hsAdar_N473D
                     /note="color: black; sequence: 
                     gcagatagacacccagatcgtaaagcaagagg; added: 2022-09-17"
     primer_bind     complement(592..623)
                     /label=2171b_hsAdar_N473D
                     /note="color: black; sequence: 
                     cctcttgctttacgatctgggtgtctatctgc; added: 2022-09-17"
     CDS             607..609
                     /label=N473D
                     /note="color: #ff00ff; direction: RIGHT"
     primer_bind     647..669
                     /label=Primer 7
                     /note="color: black; sequence: ctggtcagggggcgattccagtg; 
                     added: 2022-09-17"
     primer_bind     complement(647..669)
                     /label=Primer 8
                     /note="color: black; sequence: cactggaatcgccccctgaccag; 
                     added: 2022-09-17"
     misc_feature    652..654
                     /label=E488Q
                     /note="color: #ff0000"
     CDS             658..660
                     /label=T490A
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     708..729
                     /label=2170a_hsAdar_R510E
                     /note="color: black; sequence: gcaaggggaggagctgctcacc; 
                     added: 2022-09-17"
     primer_bind     complement(708..729)
                     /label=2170b_hsAdar_R510E
                     /note="color: black; sequence: ggtgagcagctcctccccttgc; 
                     added: 2022-09-17"
     misc_feature    718..720
                     /label=R510E
                     /note="color: #ff0000"
     primer_bind     complement(1269..1329)
                     /label=Adar_for_Aco_R
                     /note="color: black; sequence: 
                     CGACTCACTATAGTTCTAGAGGCttacgtgagtgagaactggtcctgc; added: 
                     2023-05-22"
     primer_bind     complement(1270..1309)
                     /label=2093b_hsAdar2_Rev_EcorI
                     /note="color: black; sequence: 
                     GGCTCGAGAGGCCTTGttacgtgagtgagaactggtcctg"
     primer_bind     complement(1270..1293)
                     /label=2094b
                     /note="color: black; sequence: 
                     GTATCTTATCATGTCTGGATCTACttacgtgagtgagaactggtcctg; added: 
                     2023-05-22"
     primer_bind     1302..1328
                     /label=rev_site
                     /note="color: black; sequence: 
                     CACTTATTCGTCCAACTCGAGCCTCTAGAACTATAGTGAGTC"
     misc_feature    1312..1339
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     primer_bind     complement(1336..1359)
                     /label=2094b
                     /note="color: black; sequence: 
                     GTATCTTATCATGTCTGGATCTACttacgtgagtgagaactggtcctg; added: 
                     2023-05-22"
     polyA_signal    1339..1473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(1361..1382)
                     /label=1017b
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATCAA"
     primer_bind     1543..1554
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GAAcTCCAGTCACtacGCCGCGGCGCCA"
     promoter        complement(1586..1604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1605..1617)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     CTGGATCTACtacGCGGCCGCACGCCAAGCGCGC"
     primer_bind     complement(1625..1641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    1649..1665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(1673..1703)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1673 .. 1679 / #ffffff / -10
                        2: 1680 .. 1697 / #ffffff
                        3: 1698 .. 1703 / #ffffff / -35"
     rep_origin      complement(2027..2615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2786..3646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2786 .. 3577 / #ccffcc
                        2: 3578 .. 3646 / #ccffcc / signal sequence
                     Cleavage site after base 3577"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3647..3751)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3777..4232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4306..5290
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     join(5278..5290,1..9)
                     /label=1017a
                     /note="color: black; sequence: TCGTTTAGGGAACCGCCATTCT"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgccacca tgtacccata cgatgttcca
      121 gattacgctg gaggccagct gcatttaccg caggttttag ctgacgctgt ctcacgcctg
      181 gtcctgggta agtttggtga cctgaccgac aacttctcct cccctcacgc tcgcagaaaa
      241 gtgctggctg gagtcgtcat gacaacaggc acagatgtta aagatgccaa ggtgataagt
      301 gtttctacag gaggcaaatg tattaatggt gaatacatga gtgatcgtgg ccttgcatta
      361 aatgactgcc atgcagaaat aatatctcgg agatccttgc tcagatttct ttatacacaa
      421 cttgagcttt acttaaataa caaagatgat caaaaaagat ccatctttca gaaatcagag
      481 cgaggggggt ttaggctgaa ggagaatgtc cagtttcatc tgtacatcag cacctctccc
      541 tgtggagatg ccagaatctt ctcaccacat gagccaatcc tggaagaacc agcagataga
      601 cacccaaatc gtaaagcaag aggacagcta cggaccaaaa tagagtctgg tcaggggacg
      661 attccagtgc gctccaatgc gagcatccaa acgtgggacg gggtgctgca aggggagcgg
      721 ctgctcacca tgtcctgcag tgacaagatt gcacgctgga acgtggtggg catccaggga
      781 tcactgctca gcattttcgt ggagcccatt tacttctcga gcatcatcct gggcagcctt
      841 taccacgggg accacctttc cagggccatg taccagcgga tctccaacat agaggacctg
      901 ccacctctct acaccctcaa caagcctttg ctcagtggca tcagcaatgc agaagcacgg
      961 cagccaggga aggcccccaa cttcagtgtc aactggacgg taggcgactc cgctattgag
     1021 gtcatcaacg ccacgactgg gaaggatgag ctgggccgcg cgtcccgcct gtgtaagcac
     1081 gcgttgtact gtcgctggat gcgtgtgcac ggcaaggttc cctcccactt actacgctcc
     1141 aagattacca agcccaacgt gtaccatgag tccaagctgg cggcaaagga gtaccaggcc
     1201 gccaaggcgc gtctgttcac agccttcatc aaggcggggc tgggggcctg ggtggagaag
     1261 tccaccgagc aggaccagtt ctcactcacg taacaaggcc tctcgagcct ctagaactat
     1321 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     1381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     1441 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1501 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     1561 tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     1621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     3781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4201 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     4261 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc
     4321 aatatggcgt atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc
     4381 aatatggcgt atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc
     4441 aatatggcgg acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg
     4501 gggaggggtc tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt
     4561 ccgccatatt gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat
     4621 caatatatag gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat
     4681 actatgtatt ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga
     4741 tagcccctcc caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata
     4801 gccactcccc cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg
     4861 ggcggtccta ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc
     4921 gcctggctca atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg
     4981 ctcattgccc attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc
     5041 ccacttggca gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa
     5101 ggacgccagg gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga
     5161 tggctgccaa gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt
     5221 tccattgacg taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg
     5281 tttagggaac
//
